Vsir (NM_028732) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR220924
4632428N05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Vsir |
| Synonyms | 4632428N05Rik; Dies1; PD-1H; VISTA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_028732 |
| ORF Size | 927 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_028732.4, NP_083008.1 |
| RefSeq Size | 4846 bp |
| RefSeq ORF | 930 bp |
| Locus ID | 74048 |
| UniProt ID | Q9D659 |
| Cytogenetics | 10 B4 |
| MW | 34.1 kDa |
| Summary | Immunoregulatory receptor which inhibits the T-cell response (PubMed:21383057, PubMed:24743150, PubMed:25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed:20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_028732" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC211478 | 4632428N05Rik (untagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1, (10ug) | 10 ug |
$450.00
|
|
| MG220924 | 4632428N05Rik (tGFP-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik) transcript variant 1, (10ug) | 10 ug |
$650.00
|
|
| MR220924L3 | Lenti ORF clone of 4632428N05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1 | 10 ug |
$750.00
|
|
| MR220924L4 | Lenti ORF clone of 4632428N05Rik (mGFP-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1 | 10 ug |
$750.00
|
|
Citations
FOXD3 Regulates VISTA Expression in Melanoma
Rosenbaum, Knecht, Mollaee et al
Cell Rep (2020) 30 (2), 510-524.e6 DOI: 10.1016/j.celrep.2019.12.036
Rosenbaum, Knecht, Mollaee et al
Cell Rep (2020) 30 (2), 510-524.e6 DOI: 10.1016/j.celrep.2019.12.036
Cited by 85
AI Summary: In this study, the mouse VISTA gene was amplified using the Vsir (NM_028732) expression plasmid from OriGene, which enabled the researchers to investigate the regulation and function of VISTA in melanoma cells.
Snippet: 3 and LacZ cells were previously engineered (Abel and Aplin, 2010). Transgene expression was induced with doxycycline (100ng/mL). Mouse VISTA was amplified from a Vsir (NM_028732) expression plasmid (OriGene;Rockville, MD), cloned into pENTR/D-TOPO (Invitrogen;Carlsbad, CA), and LR recombined into pLenti-4/TO/V5-DEST. Expression constructs and packaging plasmids pLP1, pLP2, pLP/VSVG were cotrans
Showing 1-1 of 1 papers.
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