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Vsir (NM_028732) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG220924
4632428N05Rik (tGFP-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik) transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $650.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vsir
Synonyms 4632428N05Rik; Dies1; PD-1H; VISTA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG220924 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGTCCCCGCGGTCCCAGAGGCCAGCAGCCCGCGCTGGGGAACCCTGCTCCTTGCTATTTTCCTGG
CTGCATCCAGAGGTCTGGTAGCAGCCTTCAAGGTCACCACTCCATATTCTCTCTATGTGTGTCCCGAGGG
ACAGAATGCCACCCTCACCTGCAGGATTCTGGGCCCCGTGTCCAAAGGGCACGATGTGACCATCTACAAG
ACGTGGTACCTCAGCTCACGAGGCGAGGTCCAGATGTGCAAAGAACACCGGCCCATACGCAACTTCACAT
TGCAGCACCTTCAGCACCACGGAAGCCACCTGAAAGCCAACGCCAGCCATGACCAGCCCCAGAAGCATGG
GCTAGAGCTAGCTTCTGACCACCACGGTAACTTCTCTATCACCCTGCGCAATGTGACCCCAAGGGACAGC
GGCCTCTACTGCTGTCTAGTGATAGAATTAAAAAACCACCACCCAGAACAACGGTTCTACGGGTCCATGG
AGCTACAGGTACAGGCAGGCAAAGGCTCGGGGTCCACATGCATGGCGTCTAATGAGCAGGACAGTGACAG
CATCACGGCTGCGGCCCTGGCCACCGGCGCCTGCATCGTGGGAATCCTCTGCCTCCCCCTTATCCTGCTG
CTGGTCTATAAGCAGAGACAGGTGGCCTCTCACCGCCGTGCCCAGGAGTTGGTGAGGATGGACAGCAGCA
ACACCCAAGGAATCGAAAACCCAGGCTTCGAGACCACTCCACCCTTCCAGGGGATGCCTGAGGCCAAGAC
CAGGCCGCCACTGTCCTATGTGGCCCAGCGGCAACCTTCGGAGTCAGGACGGTACCTGCTCTCTGACCCC
AGCACACCTCTGTCGCCTCCAGGCCCTGGGGACGTCTTTTTCCCATCCCTAGATCCAGTCCCTGACTCCC
CTAACTCTGAAGCCATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG220924 protein sequence
Red=Cloning site Green=Tags(s)

MGVPAVPEASSPRWGTLLLAIFLAASRGLVAAFKVTTPYSLYVCPEGQNATLTCRILGPVSKGHDVTIYK
TWYLSSRGEVQMCKEHRPIRNFTLQHLQHHGSHLKANASHDQPQKHGLELASDHHGNFSITLRNVTPRDS
GLYCCLVIELKNHHPEQRFYGSMELQVQAGKGSGSTCMASNEQDSDSITAAALATGACIVGILCLPLILL
LVYKQRQVASHRRAQELVRMDSSNTQGIENPGFETTPPFQGMPEAKTRPPLSYVAQRQPSESGRYLLSDP
STPLSPPGPGDVFFPSLDPVPDSPNSEAI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028732
ORF Size 927 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028732.4, NP_083008.1
RefSeq Size 4846 bp
RefSeq ORF 930 bp
Locus ID 74048
UniProt ID Q9D659
Cytogenetics 10 B4
MW 33.6 kDa
Summary Immunoregulatory receptor which inhibits the T-cell response (PubMed:21383057, PubMed:24743150, PubMed:25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed:20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_028732" in other vectors (4)
SKU Description Size Price
MC211478 4632428N05Rik (untagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1, (10ug) 10 ug
$450.00
MR220924 4632428N05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1 10 ug
$450.00
MR220924L3 Lenti ORF clone of 4632428N05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1 10 ug
$750.00
MR220924L4 Lenti ORF clone of 4632428N05Rik (mGFP-tagged) - Mouse RIKEN cDNA 4632428N05 gene (4632428N05Rik), transcript variant 1 10 ug
$750.00

Plasmid Map for MG220924

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG220924
Circular map for MG220924

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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