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Olfm2 (NM_173777) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220561
Olfm2 (Myc-DDK-tagged) - Mouse olfactomedin 2 (Olfm2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Olfm2 or NM_173777"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfm2
Synonyms A030009A06Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220561 representing NM_173777
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAAACTGAGACAGACCGGAACAACTATTGCTGGAGGTCAGACTCTCTTCCAGAGCCCGGAGGAGG
GCTGGCAGCTTTATACGTCAGCCCAGGCACCTGATGGCAAGTGCGTCTGCACAGCCGTGATCCCTGCGCA
GAGCACCTGTGCCCGAGACGGTCGGAGCAGAGAGCTTCGGCAACTCATGGAGAAGGTCCAGAATGTGTCC
CAGTCCATGGAGGTCCTTGAGCTAAGGACATTCCGGGATCTCCAGTATGTTCGCAGCATGGAGACCCTCA
TGCGGAGCCTGGATGCAAGGCTCAGGGCAGCCGATGGGTCAGTCTCAGCCAAAAGCTTTCAGGAACTGAA
GGACAGGATGACAGAGCTGCTGCCCCTGAGTTCAGTGCTGGAGCAGTACAAAGCAGACACACGAACCATT
GTGCGCCTGCGGGAGGAGGTGAGGAACCTCTCTGGCAACCTGGCTGCCATCCAAGAGGAAATGGGTGCCT
ACGGGTACGAGGACTTGCAACAGCGCGTGATGGCCCTGGAAGCCCGACTCCATGCCTGCGCGCAGAAGCT
GGGCTGCGGGAAGCTGACAGGCGTCAGTAACCCTATTACCATCCGGGCCATGGGGTCCCGCTTCGGTTCC
TGGATGACTGACACAATGGCCCCCAGTGCAGACAGCAGGGTCTGGTACATGGATGGTTATTACAAAGGCC
GCCGAGTGCTGGAGTTCCGTACTCTGGGAGACTTCATCAAAGGCCAGAACTTCATCCAGCACCTGCTGCC
ACAGCCATGGGCAGGTACGGGCCATGTGGTATACAATGGCTCTCTCTTCTACAATAAGTACCAGAGCAAT
GTGGTGGTCAAGTACCACTTCCGGTCCCGCTCGGTGCTGGTGCAGAGGAGCCTCCCGGGGGCTGGTTACA
ATAACACCTTCCCCTATTCCTGGGGCGGCTTCTCGGACATGGACTTCATGGTAGACGAGAGTGGGCTGTG
GGCTGTGTATACCACCAACCAGAATGCGGGCAACATCGTAGTCAGTCGGCTGGACCCTCACACCCTGGAG
GTCGTGAGATCCTGGGACACCGGGTACCCTAAGCGCAGCGCCGGCGAGGCCTTCATGATCTGCGGTGTCC
TCTATGTGACCAACTCTCACCTGGCCGGAGCCAAGGTCTACTTTGCGTACTTCACCAACACGTCCAGCTA
TGAGTACACGGATGTGCCCTTCCACAACCAGTATTCGCACATCTCGATGCTGGATTACAACCCCAGGGAG
CGGGCCCTGTATACCTGGAACAACGGGCACCAGGTGCTGTACAACGTCACCCTGTTCCACGTCATCAGCA
CTGCCGGGGACCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220561 representing NM_173777
Red=Cloning site Green=Tags(s)

MRKLRQTGTTIAGGQTLFQSPEEGWQLYTSAQAPDGKCVCTAVIPAQSTCARDGRSRELRQLMEKVQNVS
QSMEVLELRTFRDLQYVRSMETLMRSLDARLRAADGSVSAKSFQELKDRMTELLPLSSVLEQYKADTRTI
VRLREEVRNLSGNLAAIQEEMGAYGYEDLQQRVMALEARLHACAQKLGCGKLTGVSNPITIRAMGSRFGS
WMTDTMAPSADSRVWYMDGYYKGRRVLEFRTLGDFIKGQNFIQHLLPQPWAGTGHVVYNGSLFYNKYQSN
VVVKYHFRSRSVLVQRSLPGAGYNNTFPYSWGGFSDMDFMVDESGLWAVYTTNQNAGNIVVSRLDPHTLE
VVRSWDTGYPKRSAGEAFMICGVLYVTNSHLAGAKVYFAYFTNTSSYEYTDVPFHNQYSHISMLDYNPRE
RALYTWNNGHQVLYNVTLFHVISTAGDP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173777
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173777.4
RefSeq Size 1525 bp
RefSeq ORF 1347 bp
Locus ID 244723
UniProt ID Q8BM13
Cytogenetics 9 A3
MW 51.2 kDa
Summary Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation (By similarity). TGF-beta induces expression and nuclear translocation of OLFM2 where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HERP1 and facilitating binding of SRF to target genes (By similarity). Plays a role in AMPAR complex organization (PubMed:25218043). Is a regulator of vascular smooth-muscle cell (SMC) phenotypic switching, that acts by promoting RUNX2 and inhibiting MYOCD binding to SRF. SMC phenotypic switching is the process through which vascular SMCs undergo transition between a quiescent contractile phenotype and a proliferative synthetic phenotype in response to pathological stimuli. SMC phenotypic plasticity is essential for vascular development and remodeling (By similarity).UniProtKB/Swiss-Prot Function
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"NM_173777" in other vectors (4)
SKU Description Size Price
MC216060 Olfm2 (untagged) - Mouse olfactomedin 2 (Olfm2), (10ug) 10 ug
$503.00
MG220561 Olfm2 (tGFP-tagged) - Mouse olfactomedin 2 (Olfm2), (10ug) 10 ug
$657.00
MR220561L3 Lenti ORF clone of Olfm2 (Myc-DDK-tagged) - Mouse olfactomedin 2 (Olfm2) 10 ug
$757.00
MR220561L4 Lenti ORF clone of Olfm2 (mGFP-tagged) - Mouse olfactomedin 2 (Olfm2) 10 ug
$757.00

Plasmid Map for MR220561

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220561
Circular map for MR220561

file-alt Citations

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