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Mterf1b (NM_001042670) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219778
Gm9897 (Myc-DDK-tagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mterf1b or NM_001042670"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mterf1b
Synonyms ENSMUSG00000053178; Gm9897
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219778 representing NM_001042670
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTATGGCATCAAGAAACATCTGGTGTGTGAGAAGAAACTTTCTCTTTGATTTAAGAGGCTGGATGC
TTCAGTATTCAGCAGAAGTTTTCCTCAAGTCGATTTCATTCAGGACGTTTAGTGTGGAATGCGACAGTAA
GGACAAGGAGTCTTTGGAGGAGGAGAGGGAGGACCTGCTGAGCAACTTAGTCACCATGGGCGTTGATATC
GACATGGCAAGGAGGCGACAGCCTGGAGTTTTTAACAAGGCTGTTACGAATGAGCAGGAGCTGAAGATAT
TCCTTCTGTCCAAGGGAGCTAGTGACAAAGTGATTGGTAGCATCATATCAAGATATCCACGAGCTATAAC
ACGCACTCCTGAAAGTCTTTCAAAACGGTGGGATCTGTGGAGAAAGATTATGGCATCAGACCTTGAAATT
GTAAATATTTTGGAGCGTTCTCCTGAATCCTTTTTTCGATCTAATAACAATCTAAACTTAGAGAATAATA
TAAAGTTCCTTTGCTCTGTTGGATTGACTCATAAATGCCTCTGCAGACTGTTGACCAATGCTCCCAGAAC
CTTCTCCAACAGTCTGAATTTGAATAAGCAAATGGTTGAATTTTTGCAGGAGACTGGTATGTCCTTAGGT
CACAATGATCCCAGAGATTTTGTCAGGAAGATAATTTCTAAAAACCCTTCCATCTTAATTCAGAGCACCA
AGCGTGTAAAAACTAACATTGAATTTTTACAATCAACTTTCAACTTGAACAAACAGGACCTGCTGCTTCT
GATATGTGGCCCAGGAGCTAGAATCTTAGACCTTTCCAATGACTGTACCAAAAAAAATTACACAAACATA
AGAGAGAGGCTGCTTTCTCTTGGGTGCAGTGAGGAGGAGGTACAGAGGTTTGTCCTAAGCTATCTGAATA
TGGTCTTCTTGTCAGAAAAAAAGTTTAATGATAAAATAGACTGCCTTATAGAAGAAAAAATCAGTGCTTC
ACAAATAATTGAAAATCCGAGGATTTTAGACTCCAGCATAAATACTTTAAAAACTCGCATCCGAGAGCTG
TCCCATGCTGGGTATGACTTGAGCACATCCAGCATTGCTCTGCTGTCCTGGAGTCAGAGAAGATATGAAG
CTAAACTGAAGAGATTATGTGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219778 representing NM_001042670
Red=Cloning site Green=Tags(s)

MIMASRNIWCVRRNFLFDLRGWMLQYSAEVFLKSISFRTFSVECDSKDKESLEEEREDLLSNLVTMGVDI
DMARRRQPGVFNKAVTNEQELKIFLLSKGASDKVIGSIISRYPRAITRTPESLSKRWDLWRKIMASDLEI
VNILERSPESFFRSNNNLNLENNIKFLCSVGLTHKCLCRLLTNAPRTFSNSLNLNKQMVEFLQETGMSLG
HNDPRDFVRKIISKNPSILIQSTKRVKTNIEFLQSTFNLNKQDLLLLICGPGARILDLSNDCTKKNYTNI
RERLLSLGCSEEEVQRFVLSYLNMVFLSEKKFNDKIDCLIEEKISASQIIENPRILDSSINTLKTRIREL
SHAGYDLSTSSIALLSWSQRRYEAKLKRLCG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001042670
ORF Size 1143 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042670.1, NP_001036135.2
RefSeq Size 1368 bp
RefSeq ORF 1146 bp
Locus ID 208595
UniProt ID B9EJ57
Cytogenetics 5
MW 44 kDa
Summary Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.UniProtKB/Swiss-Prot Function
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"NM_001042670" in other vectors (5)
SKU Description Size Price
MC212505 Gm9897 (untagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MG205911 Gm9897 (tGFP-tagged) - Mouse mitochondrial transcription termination factor-like (LOC208595), nuclear gene encoding mitochondrial protein 10 ug
$657.00
MG219778 Gm9897 (tGFP-tagged) - Mouse predicted gene 9897 (Gm9897) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$657.00
MR219778L3 Lenti ORF clone of Gm9897 (Myc-DDK-tagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR219778L4 Lenti ORF clone of Gm9897 (mGFP-tagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR219778

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219778
Circular map for MR219778

file-alt Citations

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