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Mterf1b (NM_001042670) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG219778
Gm9897 (tGFP-tagged) - Mouse predicted gene 9897 (Gm9897) nuclear gene encoding mitochondrial protein, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mterf1b
Synonyms ENSMUSG00000053178; Gm9897
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG219778 representing NM_001042670
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCAAGAAACATCTGGTGTGTGAGAAGAAACTTTCTCTTTGATTTAAGAGGCTGGATGCTTCAGT
ATTCAGCAGAAGTTTTCCTCAAGTCGATTTCATTCAGGACGTTTAGTGTGGAATGCGACAGTAAGGACAA
GGAGTCTTTGGAGGAGGAGAGGGAGGACCTGCTGAGCAACTTAGTCACCATGGGCGTTGATATCGACATG
GCAAGGAGGCGACAGCCTGGAGTTTTTAACAAGGCTGTTACGAATGAGCAGGAGCTGAAGATATTCCTTC
TGTCCAAGGGAGCTAGTGACAAAGTGATTGGTAGCATCATATCAAGATATCCACGAGCTATAACACGCAC
TCCTGAAAGTCTTTCAAAACGGTGGGATCTGTGGAGAAAGATTATGGCATCAGACCTTGAAATTGTAAAT
ATTTTGGAGCGTTCTCCTGAATCCTTTTTTCGATCTAATAACAATCTAAACTTAGAGAATAATATAAAGT
TCCTTTGCTCTGTTGGATTGACTCATAAATGCCTCTGCAGACTGTTGACCAATGCTCCCAGAACCTTCTC
CAACAGTCTGAATTTGAATAAGCAAATGGTTGAATTTTTGCAGGAGACTGGTATGTCCTTAGGTCACAAT
GATCCCAGAGATTTTGTCAGGAAGATAATTTCTAAAAACCCTTCCATCTTAATTCAGAGCACCAAGCGTG
TAAAAACTAACATTGAATTTTTACAATCAACTTTCAACTTGAACAAACAGGACCTGCTGCTTCTGATATG
TGGCCCAGGAGCTAGAATCTTAGACCTTTCCAATGACTGTACCAAAAAAAATTACACAAACATAAGAGAG
AGGCTGCTTTCTCTTGGGTGCAGTGAGGAGGAGGTACAGAGGTTTGTCCTAAGCTATCTGAATATGGTCT
TCTTGTCAGAAAAAAAGTTTAATGATAAAATAGACTGCCTTATAGAAGAAAAAATCAGTGCTTCACAAAT
AATTGAAAATCCGAGGATTTTAGACTCCAGCATAAATACTTTAAAAACTCGCATCCGAGAGCTGTCCCAT
GCTGGGTATGACTTGAGCACATCCAGCATTGCTCTGCTGTCCTGGAGTCAGAGAAGATATGAAGCTAAAC
TGAAGAGATTATGTGGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG219778 representing NM_001042670
Red=Cloning site Green=Tags(s)

MASRNIWCVRRNFLFDLRGWMLQYSAEVFLKSISFRTFSVECDSKDKESLEEEREDLLSNLVTMGVDIDM
ARRRQPGVFNKAVTNEQELKIFLLSKGASDKVIGSIISRYPRAITRTPESLSKRWDLWRKIMASDLEIVN
ILERSPESFFRSNNNLNLENNIKFLCSVGLTHKCLCRLLTNAPRTFSNSLNLNKQMVEFLQETGMSLGHN
DPRDFVRKIISKNPSILIQSTKRVKTNIEFLQSTFNLNKQDLLLLICGPGARILDLSNDCTKKNYTNIRE
RLLSLGCSEEEVQRFVLSYLNMVFLSEKKFNDKIDCLIEEKISASQIIENPRILDSSINTLKTRIRELSH
AGYDLSTSSIALLSWSQRRYEAKLKRLCG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001042670
ORF Size 1137 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042670.1, NP_001036135.2
RefSeq Size 1368 bp
RefSeq ORF 1146 bp
Locus ID 208595
UniProt ID B9EJ57
Cytogenetics 5
Summary Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.UniProtKB/Swiss-Prot Function
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"NM_001042670" in other vectors (5)
SKU Description Size Price
MC212505 Gm9897 (untagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MG205911 Gm9897 (tGFP-tagged) - Mouse mitochondrial transcription termination factor-like (LOC208595), nuclear gene encoding mitochondrial protein 10 ug
$657.00
MR219778 Gm9897 (Myc-DDK-tagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $457.00
MR219778L3 Lenti ORF clone of Gm9897 (Myc-DDK-tagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR219778L4 Lenti ORF clone of Gm9897 (mGFP-tagged) - Mouse predicted gene 9897 (Gm9897), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MG219778

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG219778
Circular map for MG219778

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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