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Drosha (NM_026799) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211952
Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,174.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Drosha
Synonyms 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211952 representing NM_026799
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAGGCAATACGTGTCATAGAATGTCGTACCACCCAGGACGAGGGTGTCCCCGGGGCCGAGGAGGAC
ACGGAGCCAGACCTTCAGCACCAGCTTTCAGACCCCAAAACCTGCGACTTCTTCATCCCCAGCAGCCGCC
TGCGCAGTATCAATATGAGCCTCCGAGCGCCCCCTCTTCCTCCTACTCGAACTCTCAGGCCCCCAGCTTT
ATGCCCCCACGGCCAGACTTTGTCCCTTACCCTCCCCCAGCGGCGCCGTCTGCCCAAGGGCCTCTCCCTC
CCTGCCCAGTGAGGCCGCCTTACCCCAACCACCAGATGAGACACCCTTTCCCGGTGCCTCCCTGTTTTCC
ACCCATGCCCCCTCCGATGCCTTGTCCCAATAACCCGCCTGCCTCCGGAGCACCTCCCGGACAAGGCACT
TTCCCCTTCATGGTGCCCCCTCCTTCCATGCCCCACCCTCCGCCCCCGCCCGTCATGCCGCAGCAGGTTA
ATTACCAGTACCCCCCTGGGTACTCGCACAGCTTCCCACCGCCCGGCTTCAACAGTTACCAGAACAACTC
CAGCTCTTTCCCACCCAGTGCTAACAGCAGCAGCACTCCTCATTTTCGACACCTCCCACCATACTCACTC
CCAAAGGCTCAGAATGAGAGGCGGTCCCCAGAAAGGCTCAAGCACTACGACGACCACAGGCACCGAGATC
ACAGTCACGGGCGAGGCGAGAGGCATCGGTCCCTGGAGCGCAGGGAGCGCGGCCGCAGCCCTGAAAGGAG
AAGACCTGAGAGCCGCTACCGCTCAGACTATGATCGGGGGAGAACGCCACCGCCTCGCCACCGCAGCTAT
GAAAGGAGCAGAGAGCGGGATCGAGAGAGACACAGGCACCGGGAGGCCCGCAGATCACCGTCTCTAGAAA
GGTCCTACAAGAAAGAGTATAAGAGATCTGGAAGGAGTTACGCTTTACCAGTTGCTCCTGAGCCCGCTGG
GTGCACACCAGAGTTGCCTGGGGAGATGATTAAAACTACAGAGTCTTGGGCCCCGCCCCCGGAGAATGTG
AATCATCGTTCTCCAAGCAGGGAGAAGAAGAGAGCTCGTTGGGAGGAGGAAAAAGACAGATGGAGCGACA
GCCAGGGCTCTGGCAAAGAGAAGAACTACACGTCCATCAAAGAGAAAGAGGCAGAGGAGGTGCCTCCAGA
GAAGACGGAGGAGGAGGAGGAAGAGCTCCTTAAGCCTGTGTGGATTCGCTGTACACATTCGGAAAGCTAT
TACTCCAGTGACCCAATGGATCAGGTGGGAGACTCGACTGTCGTAGGGACAAGCAGGCTCCGTGATTTGT
ATGACAAATTTGAGGAAGAATTGGGGAATAGGCAAGAGAAGGCCAAAGCTGCCCGGCCTCCGTGGGAGCC
TCCGAAGACAAAGCTGGATGAAGATTTAGAGAGTTCCAGTGAGTCTGAATGTGAGACTGACGACGACAGC
ACCTGTTCGAGCAGCTCGGACTCGGAGGTGTTCGATGTCATTGCAGAGATTAAACGCAAAAAGGCTCACC
CTGACCGGCTTCATGATGAACTCTGGTACAACGACCCAGGCCAGATGAACGATGGACCGCTTTGCAAATG
CAGTGCAAAAGCCAGACGCACAGGAATCCGCCACAGCATTTATCCCGGAGAAGAGGCAATCAAGCCCTGC
CGTCCGATGACCAACAACGCTGGCCGGCTTTTCCACTATCGGATCACCGTCTCCCCGCCTACCAACTTCT
TAACTGACAGGCCAACAGTCATAGAATATGATGACCACGAGTATATTTTTGAAGGATTTTCTATGTTTGC
ACATGCTCCTCTGACCAATATTCCACTGTGTAAAGTGATTCGATTCAACATAGACTACACGATTCATTTT
ATCGAAGAGATGATGCCTGAGAATTTTTGTGTGAAAGGACTTGAACTGTTTTCATTGTTCCTATTCAGAG
ATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGTCCGAG
ATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGCAAAGAAGTGTTATCCATGCACCAG
ATCCTTCTCTACCTGCTGCGCTGCAGCAAGGCTCTGGTGCCCGAGGAGGAGATTGCCAACATGCTCCAGT
GGGAGGAGCTCGAGTGGCAGAAATACGCAGAGGAGTGCAAAGGCATGATCGTTACCAACCCTGGGACGAA
ACCGAGCTCTGTCCGCATTGATCAGCTGGATCGTGAACAGTTCAACCCCGAAGTGATCACTTTCCCGATT
ATCGTTCACTTTGGGATCCGCCCCGCACAGTTGAGTTATGCAGGAGACCCACAGTATCAGAAATTATGGA
AAAGCTATGTGAAACTTCGCCACCTCCTAGCAAACAGTCCCAAAGTCAAACAAACTGACAAGCAGAAGCT
GGCTCAGAGGGAGGAAGCGCTCCAGAAAATACGGCAGAAGAACACGATGCGGCGAGAAGTCACCGTGGAG
CTGAGTAGCCAAGGATTCTGGAAAACTGGCATTCGTTCTGATGTCTGTCAGCATGCAATGATGCTGCCTG
TTCTGACCCATCACATCCGGTACCATCAATGCTTAATGCACCTGGACAAGTTGATAGGATATACTTTCCA
AGACCGTTGTCTGCTCCAGCTTGCCATGACTCATCCGAGTCACCATTTAAATTTTGGAATGAATCCTGAC
CATGCCAGGAATTCTTTGTCTAACTGTGGAATTCGACAACCCAAATATGGAGACAGAAAAGTTCATCACA
TGCACATGCGGAAAAAAGGAATTAATACCCTAATAAATATCATGTCACGCCTTGGCCAAGATGATCCAAC
TCCTTCAAGGATTAATCACAACGAAAGGTTGGAGTTCCTGGGGGATGCTGTTGTTGAGTTTCTGACCAGT
GTCCACCTGTACTACCTGTTTCCTAGCTTGGAGGAAGGGGGCTTGGCGACCTATCGGACCGCCATTGTTC
AGAATCAGCACCTCGCCATGCTTGCAAAGAAACTTGAACTGGATCGATTTATGCTATATGCCCATGGGCC
CGACCTATGTAGAGAATCAGATCTCCGCCATGCGATGGCCAATTGTTTTGAAGCCTTGATAGGAGCTGTT
TACTTGGAGGGGAGCCTAGAGGAAGCCAAACAGTTATTTGGACGCTTACTCTTTAATGATCCGGACCTTC
GAGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACGGATCGGCAACTTAT
TGAAACTTCCCCAGTTCTACAGAAACTTACGGAGTTTGAAGAAGCAATTGGAGTGATCTTCACTCACGTC
CGGCTTCTGGCGAGGGCGTTCACACTGAGAACCGTGGGCTTTAACCACCTGACCCTAGGCCACAATCAGA
GGATGGAATTTCTGGGCGACTCCATAATGCAGCTGGTGGCCACAGAGTACTTGTTCATTCATTTCCCGGA
CCATCACGAAGGACACTTGACGTTGTTACGAAGTTCCTTAGTGAACAACAGAACGCAAGCCAAGGTAGCA
GAGGAACTGGGCATGCAGGAGTATGCCATCACCAACGACAAAACCAAGAGACCCGTGGCCCTAAGAACCA
AGACTTTGGCAGACCTTTTGGAATCATTTATCGCAGCGCTGTACATCGACAAGGACCTGGAATATGTCCA
CACTTTCATGAACGTCTGCTTCTTTCCCCGGCTGAAGGAGTTCATTCTGAATCAGGATTGGAACGACCCC
AAGTCGCAGCTGCAGCAGTGTTGTCTGACCCTGAGGACAGAAGGGAAAGAGCCTGACATCCCCTTATACA
AGACTCTGCAGACAGTGGGGCCATCCCATGCTAGAACCTACACTGTGGCTGTTTATTTCAAGGGAGAAAG
GATAGGCTGTGGGAAAGGACCAAGCATTCAGCAGGCGGAGATGGGAGCAGCAATGGATGCACTGGAGAAA
TATAACTTTCCCCAGATGGCCCATCAGAAGCGGTTCATTGAGCGGAAATACAGACAAGAGTTAAAGGAAA
TGAGGTGGGAAAGAGAGCATCAGGAGAGAGAGCCGGAGGAGGCTGAAGACATCAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211952 representing NM_026799
Red=Cloning site Green=Tags(s)

MQGNTCHRMSYHPGRGCPRGRGGHGARPSAPAFRPQNLRLLHPQQPPAQYQYEPPSAPSSSYSNSQAPSF
MPPRPDFVPYPPPAAPSAQGPLPPCPVRPPYPNHQMRHPFPVPPCFPPMPPPMPCPNNPPASGAPPGQGT
FPFMVPPPSMPHPPPPPVMPQQVNYQYPPGYSHSFPPPGFNSYQNNSSSFPPSANSSSTPHFRHLPPYSL
PKAQNERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPERRRPESRYRSDYDRGRTPPPRHRSY
ERSRERDRERHRHREARRSPSLERSYKKEYKRSGRSYALPVAPEPAGCTPELPGEMIKTTESWAPPPENV
NHRSPSREKKRARWEEEKDRWSDSQGSGKEKNYTSIKEKEAEEVPPEKTEEEEEELLKPVWIRCTHSESY
YSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGNRQEKAKAARPPWEPPKTKLDEDLESSSESECETDDDS
TCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPC
RPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHF
IEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQ
ILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPEVITFPI
IVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVE
LSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPD
HARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTS
VHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAV
YLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHV
RLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVA
EELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDP
KSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEK
YNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPEEAEDIKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026799
ORF Size 4119 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026799.3, NP_081075.3
RefSeq Size 4580 bp
RefSeq ORF 4122 bp
Locus ID 14000
UniProt ID Q5HZJ0
Cytogenetics 15 A1
MW 159.3 kDa
Summary Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.UniProtKB/Swiss-Prot Function
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"NM_026799" in other vectors (3)
SKU Description Size Price
MC224350 Drosha (untagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2, (10ug) 10 ug
$1,293.00
MR211952L3 Lenti ORF clone of Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2 10 ug
$1,474.00
MR211952L4 Lenti ORF clone of Drosha (mGFP-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2 10 ug
$1,474.00

Plasmid Map for MR211952

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211952
Circular map for MR211952

file-alt Citations

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