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Drosha (NM_026799) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR211952L3
Lenti ORF clone of Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,474.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Drosha
Synonyms 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR211952).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026799
ORF Size 4119 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026799.3, NP_081075.3
RefSeq Size 4580 bp
RefSeq ORF 4122 bp
Locus ID 14000
UniProt ID Q5HZJ0
Cytogenetics 15 A1
Summary Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.UniProtKB/Swiss-Prot Function
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"NM_026799" in other vectors (3)
SKU Description Size Price
MC224350 Drosha (untagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2, (10ug) 10 ug
$1,293.00
MR211952 Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2 10 ug
$589.00 $1,174.00
MR211952L4 Lenti ORF clone of Drosha (mGFP-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2 10 ug
$1,474.00

Plasmid Map for MR211952L3

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Cloning scheme for MR211952L3
Circular map for MR211952L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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