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Trim24 (NM_145076) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211536
Trim24 (Myc-DDK-tagged) - Mouse tripartite motif-containing 24 (Trim24)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $915.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trim24
Synonyms A130082H20Rik; AI447469; D430004I05Rik; Tif; TIF1; TIF1-alpha; Tif1a; TIF1alpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211536 representing NM_145076
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGCTGTGGAGAAGGCGGCGGCGGCAGCGGCTCCGGCCGGAGGCCCCGCAGCGGCGGCGCCGA
GCGGGGAGAATGAGGCCGAGAGCCGGCAGGGCCCGGACTCGGAGAGCGGCGGCGAGGCGTCCCGGCTCAA
CCTGTTGGACACTTGCGCCGTGTGCCACCAGAACATCCAGAGCCGGGTGCCCAAGCTGCTGCCCTGCCTG
CACTCGTTCTGCCAGCGCTGTTTGCCCGCGCCGCAGCGCTATCTCATGCTGACGGCGCCCGCGCTGGGCT
CGGCAGAGACCCCTCCACCCGCTCCCGCCCCCGCCCCCGCCCCGGGCTCCCCGGCCGGTGGTCCTTCGCC
ATTCGCCACCCAAGTTGGAGTCATTCGATGCCCAGTTTGCAGTCAAGAGTGTGCTGAGAGACACATCATA
GACAACTTTTTTGTGAAGGACACCACTGAAGTTCCTAGTAGTACAGTAGAAAAGTCTAATCAGGTATGTA
CAAGCTGTGAAGACAATGCAGAAGCTAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACATG
TATTAGAGCTCACCAGAGGGTGAAGTTCACAAAAGACCACACAGTCAGGCAGAAAGAAGAAGTATCTCCA
GAGGCAGTTGGGGTGACCAGTCAGCGACCAGTGTTTTGTCCCTTCCATAAAAAGGAGCAGTTGAAACTTT
ACTGTGAAACATGTGATAAACTGACCTGTCGAGACTGCCAGCTGCTAGAACACAAAGAACACAGGTATCA
ATTTATAGAAGAAGCTTTTCAGAATCAAAAAGTGATCATAGATACTCTAATCACCAAACTGATGGAAAAA
ACAAAATATATAAAGTATACAGGAAATCAGATCCAAAATAGGATAATTGAAATAAATCAAAACCAAAAGC
AGGTGGAACAGGATATTAAAGTTGCCATCTTCACATTGATGGTGGAGATAAACAAAAAAGGGAAAGCTCT
GCTGCACCAGCTTGAGAGTCTTGCAAAGGACCATCGAATGAAACTCATGCAACAACAGCAGGAAGTGGCT
GGGCTTTCTAAGCAGTTAGAGCACGTCATGCATTTTTCTAAATGGGCTGTTTCCAGTGGCAGCAGCACAG
CCTTGCTGTACAGCAAGCGGCTGATTACATACAGGTTACGGCACCTTCTTCGTGCAAGGTGTGATGCTTC
TCCTGTGACCAACACCACCATCCAGTTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATTATCAACTTG
GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAGCAGA
GTTCACAGCCACCAGGTGGTTTACCTTCCAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA
GTTACGACTCCAGCATATTCAGCAACAGGTAATGGCTCAGAGGCAACAGGTGCAACGGAGGCCAGCACCT
GTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCCAGCAGCCTTCCATCTCTCATCAGCATCCGCCAC
CACGCTTAATAAACTTTCAGAATCACAGCCCTAAGCCCAATGGACCAGTTCTTCCTCCTTATCCTCAGCA
GCTGAGATATTCACCAAGCCAGAATGTACCTCGGCAGACAACAATAAAGCCCAACCCCTTGCAAATGGCT
TTTTTGGCTCAACAGGCCATAAAACAGTGGCAGATCAGCAGTGTACAGGCTCCGCCCACAACTGCCAGCA
GCTCCTCCTCCACGCCGTCCAGCCCCACAATCACAAGTGCAGCTGGGTACGATGGAAAAGCTTTTAGTTC
ACCCATGATTGATCTGAGTGCACCGGTGGGAGGGTCTTACAATCTTCCTTCTCTTCCAGATATTGATTGT
TCAAGTACTATAATGTTGGACAACATTGCAAGGAAAGACACAGGTGTAGATCACGCCCAGCCGAGGCCTC
CGTCAAACAGAACGGTGCAGTCACCAAATTCATCAGTGCCATCTCCAGGCCTTGCAGGGCCTGTTACTAT
GACTAGCGTCCATCCCCCAATACGTTCACCTAGTGCCTCCAGTGTTGGAAGTCGAGGAAGCTCTGGCTCT
TCCAGCAAACCAGCAGGAGCTGATTCTACTCACAAGGTCCCAGTAGTCATGCTGGAGCCAATTCGAATAA
AACAGGAAAACAGTGGACCACCTGAAAATTATGATTTTCCTGTTGTTATAGTAAAACAAGAATCAGATGA
AGAATCTAGACCTCAAAATACTAACTATCCAAGAAGCATACTTACCTCCCTCCTCTTAAACAGCAGTCAG
AGCTCTGCTTCTGAGGAAACCGTGTTACGATCTGATGCCCCTGATAGTACAGGAGATCAGCCTGGACTCC
ATCAAGAAAATTCCTCAAATGGAAAGTCTGAGTGGTCGGATGCCTCCCAGAAGTCCCCTGTGCATGTCGG
AGAGACGAGGAAGGAGGATGACCCCAATGAAGACTGGTGTGCTGTTTGTCAAAATGGTGGGGAACTCCTA
TGCTGTGAGAAATGTCCTAAAGTATTCCATCTTACTTGTCATGTGCCCACCTTGACAAATTTTCCAAGTG
GAGAATGGATCTGTACTTTCTGCCGAGACTTATCTAAGCCAGAGGTTGACTATGATTGTGATGTTCCCAG
TCACCACTCAGAGAAACGGAAAAGTGAAGGCCTTACTAAGTTAACGCCAATAGACAAAAGGAAATGTGAA
CGCCTACTTCTGTTTCTTTACTGCCATGAAATGAGCCTGGCTTTCCAAGACCCTGTTCCTCTAACTGTGC
CTGATTATTATAAAATAATTAAAAACCCAATGGACTTGTCAACCATCAAGAAAAGACTTCAGGAGGATTA
TTGCATGTATACAAAGCCTGAAGACTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTC
AATGAGCCTGATTCTGAAGTAGCCAATGCTGGTATAAAACTTGAAAGCTATTTTGAAGAACTTCTAAAGA
ATCTTTATCCAGAAAAAAGGTTTCCTAAGGTAGAATTCAGGCATGAAGCAGAAGACTGTAAGTTCAGTGA
CGACTCAGACGATGACTTTGTACAGCCCCGGAAGAAGCGTCTCAAGAGCACCGAGGATCGCCAGCTGCTT
AAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211536 representing NM_145076
Red=Cloning site Green=Tags(s)

MEVAVEKAAAAAAPAGGPAAAAPSGENEAESRQGPDSESGGEASRLNLLDTCAVCHQNIQSRVPKLLPCL
HSFCQRCLPAPQRYLMLTAPALGSAETPPPAPAPAPAPGSPAGGPSPFATQVGVIRCPVCSQECAERHII
DNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSP
EAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEK
TKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVA
GLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINL
GSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQVMAQRQQVQRRPAP
VGLPNPRMQGPIQQPSISHQHPPPRLINFQNHSPKPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMA
FLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDC
SSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGS
SSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQ
SSASEETVLRSDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELL
CCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCE
RLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEF
NEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLL
K

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145076
ORF Size 3153 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145076.4, NP_659542.3
RefSeq Size 6191 bp
RefSeq ORF 3156 bp
Locus ID 21848
UniProt ID Q64127
Cytogenetics 6 B1
MW 117.1 kDa
Summary The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. provided by RefSeq, Jan 2013
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"NM_145076" in other vectors (4)
SKU Description Size Price
MC223351 Trim24 (untagged) - Mouse tripartite motif-containing 24 (Trim24), (10ug) 10 ug
$916.00
MG211536 Trim24 (tGFP-tagged) - Mouse tripartite motif protein 24 (Trim24) 10 ug
$1,115.00
MR211536L3 Lenti ORF clone of Trim24 (Myc-DDK-tagged) - Mouse tripartite motif-containing 24 (Trim24) 10 ug
$1,215.00
MR211536L4 Lenti ORF clone of Trim24 (mGFP-tagged) - Mouse tripartite motif-containing 24 (Trim24) 10 ug
$1,215.00

Plasmid Map for MR211536

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211536
Circular map for MR211536

file-alt Citations

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