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Trim24 (NM_145076) Mouse Untagged Clone

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SKU
MC223351
Trim24 (untagged) - Mouse tripartite motif-containing 24 (Trim24), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $916.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trim24
Synonyms A130082H20Rik; AI447469; D430004I05Rik; Tif; TIF1; TIF1-alpha; Tif1a; TIF1alpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223351 representing NM_145076
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGCTGTGGAGAAGGCGGCGGCGGCAGCGGCTCCGGCCGGAGGCCCCGCAGCGGCGGCGCCGA
GCGGGGAGAATGAGGCCGAGAGCCGGCAGGGCCCGGACTCGGAGAGCGGCGGCGAGGCGTCCCGGCTCAA
CCTGTTGGACACTTGCGCCGTGTGCCACCAGAACATCCAGAGCCGGGTGCCCAAGCTGCTGCCCTGCCTG
CACTCGTTCTGCCAGCGCTGTTTGCCCGCGCCGCAGCGCTATCTCATGCTGACGGCGCCCGCGCTGGGCT
CGGCAGAGACCCCTCCACCCGCTCCCGCCCCCGCCCCCGCCCCGGGCTCCCCGGCCGGTGGTCCTTCGCC
ATTCGCCACCCAAGTTGGAGTCATTCGATGCCCAGTTTGCAGTCAAGAGTGTGCTGAGAGACACATCATA
GACAACTTTTTTGTGAAGGACACCACTGAAGTTCCTAGTAGTACAGTAGAAAAGTCTAATCAGGTATGTA
CAAGCTGTGAAGACAATGCAGAAGCTAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACATG
TATTAGAGCTCACCAGAGGGTGAAGTTCACAAAAGACCACACAGTCAGGCAGAAAGAAGAAGTATCTCCA
GAGGCAGTTGGGGTGACCAGTCAGCGACCAGTGTTTTGTCCCTTCCATAAAAAGGAGCAGTTGAAACTTT
ACTGTGAAACATGTGATAAACTGACCTGTCGAGACTGCCAGCTGCTAGAACACAAAGAACACAGGTATCA
ATTTATAGAAGAAGCTTTTCAGAATCAAAAAGTGATCATAGATACTCTAATCACCAAACTGATGGAAAAA
ACAAAATATATAAAGTATACAGGAAATCAGATCCAAAATAGGATAATTGAAATAAATCAAAACCAAAAGC
AGGTGGAACAGGATATTAAAGTTGCCATCTTCACATTGATGGTGGAGATAAACAAAAAAGGGAAAGCTCT
GCTGCACCAGCTTGAGAGTCTTGCAAAGGACCATCGAATGAAACTCATGCAACAACAGCAGGAAGTGGCT
GGGCTTTCTAAGCAGTTAGAGCACGTCATGCATTTTTCTAAATGGGCTGTTTCCAGTGGCAGCAGCACAG
CCTTGCTGTACAGCAAGCGGCTGATTACATACAGGTTACGGCACCTTCTTCGTGCAAGGTGTGATGCTTC
TCCTGTGACCAACACCACCATCCAGTTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATTATCAACTTG
GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAGCAGA
GTTCACAGCCACCAGGTGGTTTACCTTCCAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA
GTTACGACTCCAGCATATTCAGCAACAGGTAATGGCTCAGAGGCAACAGGTGCAACGGAGGCCAGCACCT
GTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCCAGCAGCCTTCCATCTCTCATCAGCATCCGCCAC
CACGCTTAATAAACTTTCAGAATCACAGCCCTAAGCCCAATGGACCAGTTCTTCCTCCTTATCCTCAGCA
GCTGAGATATTCACCAAGCCAGAATGTACCTCGGCAGACAACAATAAAGCCCAACCCCTTGCAAATGGCT
TTTTTGGCTCAACAGGCCATAAAACAGTGGCAGATCAGCAGTGTACAGGCTCCGCCCACAACTGCCAGCA
GCTCCTCCTCCACGCCGTCCAGCCCCACAATCACAAGTGCAGCTGGGTACGATGGAAAAGCTTTTAGTTC
ACCCATGATTGATCTGAGTGCACCGGTGGGAGGGTCTTACAATCTTCCTTCTCTTCCAGATATTGATTGT
TCAAGTACTATAATGTTGGACAACATTGCAAGGAAAGACACAGGTGTAGATCACGCCCAGCCGAGGCCTC
CGTCAAACAGAACGGTGCAGTCACCAAATTCATCAGTGCCATCTCCAGGCCTTGCAGGGCCTGTTACTAT
GACTAGCGTCCATCCCCCAATACGTTCACCTAGTGCCTCCAGTGTTGGAAGTCGAGGAAGCTCTGGCTCT
TCCAGCAAACCAGCAGGAGCTGATTCTACTCACAAGGTCCCAGTAGTCATGCTGGAGCCAATTCGAATAA
AACAGGAAAACAGTGGACCACCTGAAAATTATGATTTTCCTGTTGTTATAGTAAAACAAGAATCAGATGA
AGAATCTAGACCTCAAAATACTAACTATCCAAGAAGCATACTTACCTCCCTCCTCTTAAACAGCAGTCAG
AGCTCTGCTTCTGAGGAAACCGTGTTACGATCTGATGCCCCTGATAGTACAGGAGATCAGCCTGGACTCC
ATCAAGAAAATTCCTCAAATGGAAAGTCTGAGTGGTCGGATGCCTCCCAGAAGTCCCCTGTGCATGTCGG
AGAGACGAGGAAGGAGGATGACCCCAATGAAGACTGGTGTGCTGTTTGTCAAAATGGTGGGGAACTCCTA
TGCTGTGAGAAATGTCCTAAAGTATTCCATCTTACTTGTCATGTGCCCACCTTGACAAATTTTCCAAGTG
GAGAATGGATCTGTACTTTCTGCCGAGACTTATCTAAGCCAGAGGTTGACTATGATTGTGATGTTCCCAG
TCACCACTCAGAGAAACGGAAAAGTGAAGGCCTTACTAAGTTAACGCCAATAGACAAAAGGAAATGTGAA
CGCCTACTTCTGTTTCTTTACTGCCATGAAATGAGCCTGGCTTTCCAAGACCCTGTTCCTCTAACTGTGC
CTGATTATTATAAAATAATTAAAAACCCAATGGACTTGTCAACCATCAAGAAAAGACTTCAGGAGGATTA
TTGCATGTATACAAAGCCTGAAGACTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTC
AATGAGCCTGATTCTGAAGTAGCCAATGCTGGTATAAAACTTGAAAGCTATTTTGAAGAACTTCTAAAGA
ATCTTTATCCAGAAAAAAGGTTTCCTAAGGTAGAATTCAGGCATGAAGCAGAAGACTGTAAGTTCAGTGA
CGACTCAGACGATGACTTTGTACAGCCCCGGAAGAAGCGTCTCAAGAGCACCGAGGATCGCCAGCTGCTT
AAGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_145076
Insert Size 3156 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145076.4, NP_659542.3
RefSeq Size 6191 bp
RefSeq ORF 3156 bp
Locus ID 21848
UniProt ID Q64127
Cytogenetics 6 B1
Summary The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. provided by RefSeq, Jan 2013
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_145076" in other vectors (4)
SKU Description Size Price
MG211536 Trim24 (tGFP-tagged) - Mouse tripartite motif protein 24 (Trim24) 10 ug
$1,115.00
MR211536 Trim24 (Myc-DDK-tagged) - Mouse tripartite motif-containing 24 (Trim24) 10 ug
$589.00 $915.00
MR211536L3 Lenti ORF clone of Trim24 (Myc-DDK-tagged) - Mouse tripartite motif-containing 24 (Trim24) 10 ug
$1,215.00
MR211536L4 Lenti ORF clone of Trim24 (mGFP-tagged) - Mouse tripartite motif-containing 24 (Trim24) 10 ug
$1,215.00

file-alt Citations

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