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Mthfd1 (NM_138745) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211246
Mthfd1 (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $857.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Other products for "Mthfd1 or NM_138745"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mthfd1
Synonyms DC; Dcs; E430024A07Rik; Mthfd
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211246 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACCAGCGGGAATCCTGAACGGGAAACTGGTTTCTGCGCAAATCAGGGATCGACTGAAGAACCAGG
TAACTCGGATGCAGGAGCAGGTACCTGGCTTCACCCCTGGCCTGGCGATCCTGCAGGTTGGCGACAGAGA
TGATTCTAATCTTTATATAAATGTGAAGCTGAAGGCTGCTGAGGAGATTGGGATCAAAGCCACTCACATT
AAATTACCAAGAACATCCACAGAGTCCGAGGTATTAAAATATGTCATCTCCCTGAATGAGGATGCCTCTG
TGCACGGATTCATAGTGCAGCTGCCTTTAGATTCAGAGAATTCCATTAACACTGAGGCAGTCATCAATGC
CATTGCACCCGAGAAGGATGTGGATGGGTTGACGAGCGTCAGTGCTGGGAAGCTCGCCAGAGGTGATCTA
AATGACTGCTTCATCCCATGCACACCCAAGGGATGCTTGGAACTCATCAAAGAGGCAGGGGTGCAGATTG
CCGGAAGGCACGCGGTGGTGGTTGGTCGAAGTAAGATAGTTGGTGCACCCATGCATGACCTCCTTCTGTG
GAACAATGCCACGGTGACCACCTGCCACTCCAAGACTGCCAATCTGGACAAGGAGGTAAATAAAGGAGAC
ATTCTGGTGGTTGCCACAGGCCAGCCTGAGATGGTGAAGGGGGAGTGGATCAAGCCTGGGGCTGTGGTCA
TAGACTGTGGGATCAATTATGTTCCAGATGATACAAAACCAAATGGAAGGAAAGTCGTGGGTGATGTGGC
CTACGATGAGGCCAAGGAGAGGGCGAGCTTTATCACGCCTGTCCCTGGTGGCGTGGGGCCCATGACCGTG
GCAATGCTGATGCAGAGCACAGTAGAGAGCGCACAGCGCTTCCTACAGAAATTTAAGCCAGGGAAGTGGA
CAATTCAGTATAACAAGCTGAACCTCAAGACTCCTGTCCCAAGTGACATTGCTATATCACGATCTTGCAA
ACCAAAGCTCATCGGGAACTTGGCCCGAGAAATCGGGCTGCTCACCGAAGAGGTGGAGTTGTACGGGGAA
ACGAAGGCTAAAGTCTTGCTGTCAGCATTAGATCGCCTGAAGTATCAGCCAGATGGGAAATATGTCGTGG
TGACTGGAATTACTCCAACACCGCTGGGAGAAGGGAAGAGTACCACCACGATCGGGCTGGTGCAGGCCCT
CGGCGCCCACCTGCGACAGAACGTCTTTGCGTGTGTGCGACAGCCTTCGCAGGGCCCCACCTTTGGGATA
AAAGGTGGCGCTGCAGGAGGCGGCTATTCCCAGGTCATTCCAATGGAAGAGTTTAACCTCCACCTCACTG
GTGACATCCACGCCATCACTGCTGCTAACAACCTCGTGGCTGCTGCCATTGATGCTCGGATATTTCATGA
GCTGACCCAGACAGACAAGGCTCTCTTTAATCGTCTGGTACCTTCAGTAAACGGAATAAGAAAATTCTCT
GACATCCAAATCCGCAGGTTACGGAGGCTAGGCATTGAGAAGACTGACCCCACCACGCTGACAGATGACG
AGATAAACAGATTTGCAAGACTGGATATTGACCCAGAAACCATAACATGGCAGAGAGTGTTGGACACCAA
CGATAGATTCCTGAGGAAGATCACAATTGGACAGTCTCCAACAGAGAAAGGCCACACACGCACGGCCCAG
TTTGATATCTCTGTGGCCAGTGAGATCATGGCAGTCCTGGCCCTCACGAGCTCTCTGGAAGACATGAGAG
AGAGACTGGGCAGAATGGTGGTGGCGTCCAGTAAGAAAGGGGAGCCTATCAGTTGCGAAGACCTGGGCGT
GAGTGGGACGCTGACGGTGCTGATGAAGGATGCAATCAAGCCCAACCTCATGCAGACCTTAGAGGGCACG
CCAGTGTTTGTCCATGCCGGGCCTTTCGCCAACATTGCACACGGGAATTCCTCCATCATTGCAGACCGGA
TTGCACTCAAGCTGGTAGGCCCTGAGGGCTTCGTAGTGACGGAAGCTGGATTCGGAGCAGACATAGGAAT
GGAAAAGTTCTTCAACATCAAGTGCCGGTATTCTGGTCTCCAGCCACACGTGGTGGTTCTGGTTGCCACT
GTCAGGGCCCTTAAGATGCACGGGGGTGGCCCCACGGTCACCGCTGGACTACCTCTTCCCAAGGCTTACA
CAGAAGAGGACCTGGACCTGGTTGAAAAGGGCTTCAGTAACTTGAGGAAACAAATCGAAAATGCTAGAAT
GTTTGGAGTCCCTGTCGTCGTGGCTGTGAACGTGTTCAAGACAGATACAGATGCTGAGCTGGACCTTGTC
AGCCGCCTCTCCAGAGAACACGGGGCTTTTGATGCTGTCAAGTGCACCCACTGGGCAGAAGGGGGCCAGG
GGGCCTTGGCCCTGGCTCAGGCTGTCCAGAGAGCGTCACAGGCCCCCAGCAGCTTCCAGCTCCTCTATGA
CCTCAAGCTCTCAATTGAGGATAAAATCAGGATTATTGCACAGAGGATCTACGGGGCCGATGACATCGAA
TTGCTCCCGGAAGCACAGAACAAAGCAGAAATTTACACAAAGCAGGGCTTTGGGAATCTACCCATCTGCA
TGGCCAAAACACACTTGTCCTTGTCTCACAACCCAGAGCAAAAAGGGGTGCCTACTGGTTTCGTTCTGCC
CATCCGGGACATCCGTGCCAGCGTTGGGGCAGGTTTCCTGTATCCTTTAGTAGGAACGATGAGCACAATG
CCTGGACTCCCTACGAGGCCCTGTTTTTATGATATTGATTTGGATCCTGAAACGGAACAAGTGAATGGAT
TGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211246 protein sequence
Red=Cloning site Green=Tags(s)

MAPAGILNGKLVSAQIRDRLKNQVTRMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAEEIGIKATHI
KLPRTSTESEVLKYVISLNEDASVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSVSAGKLARGDL
NDCFIPCTPKGCLELIKEAGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDKEVNKGD
ILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTV
AMLMQSTVESAQRFLQKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGE
TKAKVLLSALDRLKYQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLRQNVFACVRQPSQGPTFGI
KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGIRKFS
DIQIRRLRRLGIEKTDPTTLTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQSPTEKGHTRTAQ
FDISVASEIMAVLALTSSLEDMRERLGRMVVASSKKGEPISCEDLGVSGTLTVLMKDAIKPNLMQTLEGT
PVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVAT
VRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAVNVFKTDTDAELDLV
SRLSREHGAFDAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSIEDKIRIIAQRIYGADDIE
LLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTM
PGLPTRPCFYDIDLDPETEQVNGLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138745
ORF Size 2805 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138745.2
RefSeq Size 3324 bp
RefSeq ORF 2808 bp
Locus ID 108156
UniProt ID Q922D8
Cytogenetics 12 C3
MW 101.3 kDa
Summary This gene encodes a trifunctional cytoplasmic enzyme. The encoded protein functions as a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase, and a formyltetrahydrofolate synthase. The encoded enzyme functions in de novo synthesis of purines and thymidylate and in regeneration of methionine from homocysteine. provided by RefSeq, Oct 2009
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Documents
Resources
"NM_138745" in other vectors (4)
SKU Description Size Price
MC200495 Mthfd1 (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), (10ug) 10 ug
$857.00
MG211246 Mthfd1 (tGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase 10 ug
$1,057.00
MR211246L3 Lenti ORF clone of Mthfd1 (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1) 10 ug
$1,157.00
MR211246L4 Lenti ORF clone of Mthfd1 (mGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1) 10 ug
$1,157.00

Plasmid Map for MR211246

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211246
Circular map for MR211246

file-alt Citations

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