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Mthfd1 (NM_138745) Mouse Untagged Clone

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SKU
MC200495
Mthfd1 (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $857.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mthfd1
Synonyms DC; Dcs; E430024A07Rik; Mthfd
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC008523 sequence for NM_138745
TCCCTGAGCACGTGGGTTGTGCTGTCCTTCGCGCGCGGAGCGACTGGAACCTGATTTTTGCTGGTGTTCA CCGTGGGTGGCAGACTAGCAAAGGCCATGGCACCAGCGGGAATCCTGAACGGGAAACTGGTTTCTGCGCA AATCAGGGATCGACTGAAGAACCAGGTAACTCGGATGCAGGAGCAGGTACCTGGCTTCACCCCTGGCCTG GCGATCCTGCAGGTTGGCGACAGAGATGATTCTAATCTTTATATAAATGTGAAGCTGAAGGCTGCTGAGG AGATTGGGATCAAAGCCACTCACATTAAATTACCAAGAACATCCACAGAGTCCGAGGTATTAAAATATGT CATCTCCCTGAATGAGGATGCCTCTGTGCACGGATTCATAGTGCAGCTGCCTTTAGATTCAGAGAATTCC ATTAACACTGAGGCAGTCATCAATGCCATTGCACCCGAGAAGGATGTGGATGGGTTGACGAGCGTCAGTG CTGGGAAGCTCGCCAGAGGTGATCTAAATGACTGCTTCATCCCATGCACACCCAAGGGATGCTTGGAACT CATCAAAGAGGCAGGGGTGCAGATTGCCGGAAGGCACGCGGTGGTGGTTGGTCGAAGTAAGATAGTTGGT GCACCCATGCATGACCTCCTTCTGTGGAACAATGCCACGGTGACCACCTGCCACTCCAAGACTGCCAATC TGGACAAGGAGGTAAATAAAGGAGACATTCTGGTGGTTGCCACAGGCCAGCCTGAGATGGTGAAGGGGGA GTGGATCAAGCCTGGGGCTGTGGTCATAGACTGTGGGATCAATTATGTTCCAGATGATACAAAACCAAAT GGAAGGAAAGTCGTGGGTGATGTGGCCTACGATGAGGCCAAGGAGAGGGCGAGCTTTATCACGCCTGTCC CTGGTGGCGTGGGGCCCATGACCGTGGCAATGCTGATGCAGAGCACAGTAGAGAGCGCACAGCGCTTCCT ACAGAAATTTAAGCCAGGGAAGTGGACAATTCAGTATAACAAGCTGAACCTCAAGACTCCTGTCCCAAGT GACATTGCTATATCACGATCTTGCAAACCAAAGCTCATCGGGAACTTGGCCCGAGAAATCGGGCTGCTCA CCGAAGAGGTGGAGTTGTACGGGGAAACGAAGGCTAAAGTCTTGCTGTCAGCATTAGATCGCCTGAAGTA TCAGCCAGATGGGAAATATGTCGTGGTGACTGGAATTACTCCAACACCGCTGGGAGAAGGGAAGAGTACC ACCACGATCGGGCTGGTGCAGGCCCTCGGCGCCCACCTGCGACAGAACGTCTTTGCGTGTGTGCGACAGC CTTCGCAGGGCCCCACCTTTGGGATAAAAGGTGGCGCTGCAGGAGGCGGCTATTCCCAGGTCATTCCAAT GGAAGAGTTTAACCTCCACCTCACTGGTGACATCCACGCCATCACTGCTGCTAACAACCTCGTGGCTGCT GCCATTGATGCTCGGATATTTCATGAGCTGACCCAGACAGACAAGGCTCTCTTTAATCGTCTGGTACCTT CAGTAAACGGAATAAGAAAATTCTCTGACATCCAAATCCGCAGGTTACGGAGGCTAGGCATTGAGAAGAC TGACCCCACCACGCTGACAGATGACGAGATAAACAGATTTGCAAGACTGGATATTGACCCAGAAACCATA ACATGGCAGAGAGTGTTGGACACCAACGATAGATTCCTGAGGAAGATCACAATTGGACAGTCTCCAACAG AGAAAGGCCACACACGCACGGCCCAGTTTGATATCTCTGTGGCCAGTGAGATCATGGCAGTCCTGGCCCT CACGAGCTCTCTGGAAGACATGAGAGAGAGACTGGGCAGAATGGTGGTGGCGTCCAGTAAGAAAGGGGAG CCTATCAGTTGCGAAGACCTGGGCGTGAGTGGGACGCTGACGGTGCTGATGAAGGATGCAATCAAGCCCA ACCTCATGCAGACCTTAGAGGGCACGCCAGTGTTTGTCCATGCCGGGCCTTTCGCCAACATTGCACACGG GAATTCCTCCATCATTGCAGACCGGATTGCACTCAAGCTGGTAGGCCCTGAGGGCTTCGTAGTGACGGAA GCTGGATTCGGAGCAGACATAGGAATGGAAAAGTTCTTCAACATCAAGTGCCGGTATTCTGGTCTCCAGC CACACGTGGTGGTTCTGGTTGCCACTGTCAGGGCCCTTAAGATGCACGGGGGTGGCCCCACGGTCACCGC TGGACTACCTCTTCCCAAGGCTTACACAGAAGAGGACCTGGACCTGGTTGAAAAGGGCTTCAGTAACTTG AGGAAACAAATCGAAAATGCTAGAATGTTTGGAGTCCCTGTCGTCGTGGCTGTGAACGTGTTCAAGACAG ATACAGATGCTGAGCTGGACCTTGTCAGCCGCCTCTCCAGAGAACACGGGGCTTTTGATGCTGTCAAGTG CACCCACTGGGCAGAAGGGGGCCAGGGGGCCTTGGCCCTGGCTCAGGCTGTCCAGAGAGCGTCACAGGCC CCCAGCAGCTTCCAGCTCCTCTATGACCTCAAGCTCTCAATTGAGGATAAAATCAGGATTATTGCACAGA GGATCTACGGGGCCGATGACATCGAATTGCTCCCGGAAGCACAGAACAAAGCAGAAATTTACACAAAGCA GGGCTTTGGGAATCTACCCATCTGCATGGCCAAAACACACTTGTCCTTGTCTCACAACCCAGAGCAAAAA GGGGTGCCTACTGGTTTCGTTCTGCCCATCCGGGACATCCGTGCCAGCGTTGGGGCAGGTTTCCTGTATC CTTTAGTAGGAACGATGAGCACAATGCCTGGACTCCCTACGAGGCCCTGTTTTTATGATATTGATTTGGA TCCTGAAACGGAACAAGTGAATGGATTGTTTTAAGCAGATCTTCCATCTCCAAGAGGCCACTCTGTCCGG CCAGTGTCTGTTCAGGCCCACTGAAGAAGTGTGCAGAAGTCTTGGAAGTCTGTCCCCGCCCTGAAGAGCT TCAGAAATAGTGGGAGTTTCCCTAAAGCCTTTCATAGCCTTAATTCACGTCATGTATAAATTAACATAAA TCATGTCTATTTACATAGTGAAGGTCCAGAATAAATGAAATAATTTTGCTAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_138745
Insert Size 2808 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC008523, AAH08523
RefSeq Size 3159 bp
RefSeq ORF 2808 bp
Locus ID 108156
UniProt ID Q922D8
Cytogenetics 12 C3
Summary This gene encodes a trifunctional cytoplasmic enzyme. The encoded protein functions as a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase, and a formyltetrahydrofolate synthase. The encoded enzyme functions in de novo synthesis of purines and thymidylate and in regeneration of methionine from homocysteine. provided by RefSeq, Oct 2009
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Documents
Resources
"NM_138745" in other vectors (4)
SKU Description Size Price
MG211246 Mthfd1 (tGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase 10 ug
$1,057.00
MR211246 Mthfd1 (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1) 10 ug
$589.00 $857.00
MR211246L3 Lenti ORF clone of Mthfd1 (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1) 10 ug
$1,157.00
MR211246L4 Lenti ORF clone of Mthfd1 (mGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1) 10 ug
$1,157.00

file-alt Citations

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