PCMV6-Kan/Neo Mammalian Expression Vector

Snippet: WT-GFP) and PKCγ-A24E (pCMV-PKCγ-A24E-GFP for HEK293T cells and pL7- PKCγ-A24E-mGFP for PCs) constructs were previously described [18]. Mouse Metastasis suppressor 1 (Mtss1) transcript variant 1 (Origene, MC201978) in a tag-free pCMV-Kan/Neo (PCMV6KN) vector was amplified by PCR using CloneAmp HiFi PCR Premix (Takara, 639,298) and primers Mtss1 pL7 (NotI) forward: 5' TAT TGC GGC CGC ATG GAG GC

Snippet: constructs, primers and silencing RNA (siRNA) The expression vector encoding human DNAJB3 (pCMV-DNAJB3;#RC205526), AKT (pCMV-AKT1;#R220257) and the pCMV empty vector (#pCMV6KN) were purchased from OriGene (OriGene Technologies, Inc, Rockville, MD). Plasmids encoding hDNAJB3 as a C-terminal fusion with GST (pGK-DNAJB3) and 6xHIS residues (pH6K-DNAJB3) were made by digesting pCMV-DNAJB3 with BglI

Snippet: For gene expression studies, we transfected the cells with Hnf1α-expressing plasmid (MC202766;OriGene Technologies, USA), control plasmid (PCMV6KN;OriGene Technologies) or Glut2-expressing plasmid (MG208388;OriGene Technologies) using TurboFectin transfection reagent (TF81001;OriGene Technologies) according to the

Snippet: … In addition, nuclear PKM2 also can work as a transcriptional regulator to activate HIF-1α [18], β-catenin [11,19], MEK5 [20], and Oct-4 [21], which promotes cell proliferation and tumor;pCMV6KN empty vector from ORIGENE and pcDNA3.1(+) empty vector from GeneScript were used to clone ENST00000389093 and ENST00000568883 transcripts

Snippet: Cells were transfected with either overexpression plasmid GPR109A (#MC211930;HCAR2, Origene), or shRNA GPR109A (#TR511005;HCAR2‐sh, Origene), or control vector (PCMV6KN, Origene) using Lipofectamine 3000;;- Cells were transfected with either overexpression plasmid GPR109A (#MC211930;HCAR2‐sh, Origene), or control vector (PCMV6KN, Origene) using Lipofec- tamine 3000