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Diap3 (BC028920) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211218
Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $851.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Diap3 or BC028920"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Diap3
Synonyms Dia2, mDia2, p134MDia2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211218 representing BC028920
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGATCCCAGGCAGCCCGCTATGATGGCAG
ACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAAAGCATCCTTGAAGAAGTGTTAGAAGC
CTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTTCCATTGTGGAAGGCCTCCGGCATAAC
TCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCTCGTTACATCTCCTGATGATTTGGACT
TCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAACTTAAAGGGCAT
TAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGCACAAAGAAGAAGATTTGAGTGAGTTT
TTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATCTGATGTTTACAGCATGTTATGGGACA
CAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTTCAGCATCTCCTGCTCATTCGCAATGA
TCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTGTGTCACAGATTGTGTTACATAGAGAT
GGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGACTTAAGTCAGTTTGTAGATGTTTGCA
TAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAACATTGCAAGAAATTTGAAAAAGAGTG
TACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGGCAAAGATTAATGAGCTTCAAGCAGAG
TTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTACAAAAATTCCTTTGCAACCTTCAGTAG
AAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCACTCAGTGGAGGAGTGCCGCCTCCCCC
ACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAATGCCATTTGGTGGCCCTGTACCACCA
CCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATTAAACCTGCCATTTGGTTTGAAACCAA
AGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCGGACCAAATGAAATGTC
TGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAAATAGGGATTTGCTTTGTAAACTTGAG
AACACTTTTTGTTGCCAAGACAAAGAGAAAAGGAATACAAATGACTTTGATGAGAAGAAAGTTATTAAGA
AGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAGAACCTTTCAATCTTCCTGAGCTCCTT
CCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGGATGAAACACAGTTGTCAGAGTCCATG
ATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAAGTCATTGTCCCAGTTTAGAAGTGACT
ATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGCAATGTGAAGAGACTCCGGCCACGGCT
CAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC
AGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAAGTTGCTGGAACTTGTGTTGCTAATGG
GAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTGACCTTAGCTCTCTCTGTAAACT
GAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATTTCCTGGTAGATGTATGTGAAGAAAAG
CATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGACAAAGCTAGCAGAGTCTCTGTAGAAA
TGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGAATTTGGAAACCTTTCC
CCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCAGCTTCGTTATCAGTGCGAACGAGCAG
TATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTACCAGAGTATAATGGGCTACTATGCTG
TCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTGAACAACTTCAGAACTTCATTTATGCT
AGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGGAGAAACGTGCCAGGATAGCGAAAGAG
CGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCGCTTACTAGAAATGAAAACTGAGGGAG
ATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCAGGGGCTGCCTTCCGCGACAGAAGAAA
AAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGATGTCTCAGAGGCCTGTTCTCAAAGTT
TGTAACCATGAAAATCAGAAAATGCAGTTGACAGAAGGGTCACGTCCACACCACAGTATCAATTGCAACT
CCACCAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGACACTCATGCGTCTACAGGGAGGATCAA
GGCAGTTGAGAAGGAAGCCTGTAATGCAGAAAGCAACAGAAAAAAGGAAATGGAACTTCTTGGCTCTGTT
GCTAAAAGCGAATCAGTTCCTGAAGTTGAAGCCCTGCTGGCAAGATTACGAGCTTTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211218 representing BC028920
Red=Cloning site Green=Tags(s)

MSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEESILEEVLEALTSAGEERKIDRFFSIVEGLRHN
SVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLKEILPNLKGIKNDGLDIQLKVFDEHKEEDLSEF
FHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSILQHLLLIRNDRFIREQYFKLIDECVSQIVLHRD
GTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASEHCKKFEKECTDHQETQAQLQKREAKINELQAE
LQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPALSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPP
PPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNWLKIGPNEMSENCFWIKVNENKYENRDLLCKLE
NTFCCQDKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQNLSIFLSSFRVPYEKIRTMILEVDETQLSESM
IQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAV
STACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFGFDLSSLCKLKDTKSADQKTTLLHFLVDVCEEK
HADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQLEKNLETFPPPEDLHDKFVIKMSSFVISANEQ
YEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDLNNFRTSFMLALKENIKKREAAEKEKRARIAKE
RAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQSGAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKV
CNHENQKMQLTEGSRPHHSINCNSTRTPVAKELNYNLDTHASTGRIKAVEKEACNAESNRKKEMELLGSV
AKSESVPEVEALLARLRAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC028920
ORF Size 2787 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC028920.1
RefSeq Size 3770 bp
RefSeq ORF 2789 bp
Locus ID 56419
Cytogenetics 14 E1
MW 138.2 kDa
Summary Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).UniProtKB/Swiss-Prot Function
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Resources
"BC028920" in other vectors (4)
SKU Description Size Price
MC220799 Diap3 (untagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236), (10ug) 10 ug
$937.00
MG211218 Diap3 (tGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) 10 ug
$1,051.00
MR211218L3 Lenti ORF clone of Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) 10 ug
$1,151.00
MR211218L4 Lenti ORF clone of Diap3 (mGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) 10 ug
$1,151.00

Plasmid Map for MR211218

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211218
Circular map for MR211218

file-alt Citations

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