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Diap3 (BC028920) Mouse Untagged Clone

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SKU
MC220799
Diap3 (untagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $937.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Diap3
Synonyms Dia2, mDia2, p134MDia2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC028920
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGATCCCAGGCAGCCCGCTATGATGGCAG
ACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAAAGCATCCTTGAAGAAGTGTTAGAAGC
CTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTTCCATTGTGGAAGGCCTCCGGCATAAC
TCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCTCGTTACATCTCCTGATGATTTGGACT
TCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAACTTAAAGGGCAT
TAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGCACAAAGAAGAAGATTTGAGTGAGTTT
TTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATCTGATGTTTACAGCATGTTATGGGACA
CAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTTCAGCATCTCCTGCTCATTCGCAATGA
TCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTGTGTCACAGATTGTGTTACATAGAGAT
GGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGACTTAAGTCAGTTTGTAGATGTTTGCA
TAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAACATTGCAAGAAATTTGAAAAAGAGTG
TACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGGCAAAGATTAATGAGCTTCAAGCAGAG
TTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTACAAAAATTCCTTTGCAACCTTCAGTAG
AAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCACTCAGTGGAGGAGTGCCGCCTCCCCC
ACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAATGCCATTTGGTGGCCCTGTACCACCA
CCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATTAAACCTGCCATTTGGTTTGAAACCAA
AGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCGGACCAAATGAAATGTC
TGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAAATAGGGATTTGCTTTGTAAACTTGAG
AACACTTTTTGTTGCCAAGACAAAGAGAAAAGGAATACAAATGACTTTGATGAGAAGAAAGTTATTAAGA
AGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAGAACCTTTCAATCTTCCTGAGCTCCTT
CCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGGATGAAACACAGTTGTCAGAGTCCATG
ATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAAGTCATTGTCCCAGTTTAGAAGTGACT
ATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGCAATGTGAAGAGACTCCGGCCACGGCT
CAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC
AGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAAGTTGCTGGAACTTGTGTTGCTAATGG
GAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTGACCTTAGCTCTCTCTGTAAACT
GAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATTTCCTGGTAGATGTATGTGAAGAAAAG
CATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGACAAAGCTAGCAGAGTCTCTGTAGAAA
TGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGAATTTGGAAACCTTTCC
CCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCAGCTTCGTTATCAGTGCGAACGAGCAG
TATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTACCAGAGTATAATGGGCTACTATGCTG
TCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTGAACAACTTCAGAACTTCATTTATGCT
AGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGGAGAAACGTGCCAGGATAGCGAAAGAG
CGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCGCTTACTAGAAATGAAAACTGAGGGAG
ATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCAGGGGCTGCCTTCCGCGACAGAAGAAA
AAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGATGTCTCAGAGGCCTGTTCTCAAAGTT
TGTAACCATGAAAATCAGAAAATGCAGTTGACAGAAGGGTCACGTCCACACCACAGTATCAATTGCAACT
CCACCAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGACACTCATGCGTCTACAGGGAGGATCAA
GGCAGTTGAGAAGGAAGCCTGTAATGCAGAAAGCAACAGAAAAAAGGAAATGGAACTTCTTGGCTCTGTT
GCTAAAAGCGAATCAGTTCCTGAAGTTGAAGCCCTGCTGGCAAGATTACGAGCTTTATAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC028920
Insert Size 2790 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC028920, AAH28920
RefSeq Size 3770 bp
RefSeq ORF 2789 bp
Locus ID 56419
Cytogenetics 14 E1
Summary Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC028920" in other vectors (4)
SKU Description Size Price
MG211218 Diap3 (tGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) 10 ug
$1,051.00
MR211218 Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) 10 ug
$589.00 $851.00
MR211218L3 Lenti ORF clone of Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) 10 ug
$1,151.00
MR211218L4 Lenti ORF clone of Diap3 (mGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) 10 ug
$1,151.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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