Dnmt3a (NM_007872) Mouse Tagged ORF Clone

SKU MR211146

DNMT3A (Myc-DDK-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Dnmt3a
• Synonyms: MmuIIIA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211146 representing NM_007872; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCCTCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAG; AGGAACAGGAGGAGAACCGTGGCAAGGAAGAGCGCCAGGAGCCCAGCGCCACGGCCCGGAAGGTGGGGAG; GCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAGCAGTGACACCCCCAAGGACCCAGCAGTGACCACC; AAGTCTCAGCCCATGGCCCAGGACTCTGGCCCCTCAGATCTGCTACCCAATGGAGACTTGGAGAAGCGGA; GTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAGGGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGG; AACTGAGACCCCACCAGAAGCCTCCAGAGCTGTGGAGAATGGCTGCTGTGTGACCAAGGAAGGCCGTGGA; GCCTCTGCAGGAGAGGGCAAAGAACAGAAGCAGACCAACATCGAATCCATGAAAATGGAGGGCTCCCGGG; GCCGACTGCGAGGTGGCTTGGGCTGGGAGTCCAGCCTCCGTCAGCGACCCATGCCAAGACTCACCTTCCA; GGCAGGGGACCCCTACTACATCAGCAAACGGAAACGGGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCT; GAGAAGAAAGCCAAGGTAATTGCAGTAATGAATGCTGTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGA; AGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGCCCACGGACCCTGCTTCTCCGACTGTGGCCACCAC; CCCTGAGCCAGTAGGAGGGGATGCTGGGGACAAGAATGCTACCAAAGCAGCCGACGATGAGCCTGAGTAT; GAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTGTGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAG; GCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCCGAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTT; CGGAGATGGCAAGTTCTCAGTGGTGTGTGTGGAGAAGCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTC; CACCAGGCCACCTACAACAAGCAGCCCATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCA; GCCGTGCCGGGAAGCTGTTTCCAGCTTGCCATGACAGTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGT; GCAGAACAAGCAGATGATTGAATGGGCCCTCGGTGGCTTCCAGCCCTCGGGTCCTAAGGGCCTGGAGCCA; CCAGAAGAAGAGAAGAATCCTTACAAGGAAGTTTACACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTT; ACGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGAGCACAACAGAGAAACCTAAGGTCAAGGAGATCAT; TGATGAGCGCACAAGGGAGCGGCTGGTGTATGAGGTGCGCCAGAAGTGCAGAAACATCGAGGACATTTGT; ATCTCATGTGGGAGCCTCAATGTCACCCTGGAGCACCCACTCTTCATTGGAGGCATGTGCCAGAACTGTA; AGAACTGCTTCTTGGAGTGTGCTTACCAGTATGACGACGATGGGTACCAGTCCTATTGCACCATCTGCTG; TGGGGGGCGTGAAGTGCTCATGTGTGGGAACAACAACTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGAT; CTCTTGGTGGGGCCAGGAGCTGCTCAGGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGC; ATAAGGGCACCTATGGGCTGCTGCGAAGACGGGAAGACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAA; TAACCATGACCAGGAATTTGACCCCCCAAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATC; CGCGTGCTGTCTCTCTTTGATGGGATTGCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGG; ACCGCTACATTGCCTCCGAGGTGTGTGAGGACTCCATCACGGTGGGCATGGTGCGGCACCAGGGAAAGAT; CATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGGAGTGGGGCCCATTCGACCTGGTG; ATTGGAGGCAGTCCCTGCAATGACCTCTCCATTGTCAACCCTGCCCGCAAGGGACTTTATGAGGGTACTG; GCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT; CTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGCGATTTCTTGAG; TCTAACCCCGTGATGATTGACGCCAAAGAAGTGTCTGCTGCACACAGGGCCCGTTACTTCTGGGGTAACC; TTCCTGGCATGAACAGGCCTTTGGCATCCACTGTGAATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCA; CGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCATTACCACCAGGTCAAACTCTATAAAGCAGGGCAAA; GACCAGCATTTCCCCGTCTTCATGAACGAGAAGGAGGACATCCTGTGGTGCACTGAAATGGAAAGGGTGT; TTGGCTTCCCCGTCCACTACACAGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCG; ATCGTGGAGCGTGCCGGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211146 representing NM_007872; Red=Cloning site Green=Tags(s); ; MPSSGPGDTSSSSLEREDDRKEGEEQEENRGKEERQEPSATARKVGRPGRKRKHPPVESSDTPKDPAVTT; KSQPMAQDSGPSDLLPNGDLEKRSEPQPEEGSPAAGQKGGAPAEGEGTETPPEASRAVENGCCVTKEGRG; ASAGEGKEQKQTNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREA; EKKAKVIAVMNAVEENQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGGDAGDKNATKAADDEPEY; EDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAF; HQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDSGKAVEVQNKQMIEWALGGFQPSGPKGLEP; PEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDIC; ISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVD; LLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPI; RVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLV; IGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLE; SNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGK; DQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007872
• ORF Size: 2724 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007872.4, NP_031898.1
• RefSeq Size: 9735 bp
• RefSeq ORF: 2727 bp
• Locus ID: 13435
• UniProt ID: O88508
• Cytogenetics: 12 A1.1
• MW: 102.1 kDa
• Summary: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]