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Dnmt3a (NM_007872) Mouse Untagged Clone

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SKU
MC200622
Dnmt3a (untagged) - Mouse DNA methyltransferase 3A (Dnmt3a), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $832.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dnmt3a
Synonyms MmuIIIA
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC007466 sequence for NM_007872
CCCACGCGTCCGCCCACGCGTCCGCGCCGCGGCAGCAGGGCGAGCCGGGCCGCCCGACCCGCCGGGCCAC ACGGCAGAGCCGCCTGAAGCCCAGCGCTGAGGCTGCACTTTTCCGAGGGCTTGACATCAGGGTCTATGTT TAAGTCTTAGCTCTTGCTTACAAAGACCACGGCAATTCCTTCTCTGAAGCCCTCGCAGCCCCACAGCGCC CTCGCAGCCCCAGCCTGCCGCCTACTGCCCAGCAATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCC TCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAGAGGAACAGGAGGAGAACCGTGGCAAGGAAGAGCGCC AGGAGCCCAGCGCCACGGCCCGGAAGGTGGGGAGGCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAG CAGTGACACCCCCAAGGACCCAGCAGTGACCACCAAGTCTCAGCCCATGGCCCAGGACTCTGGCCCCTCA GATCTGCTACCCAATGGAGACTTGGAGAAGCGGAGTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAG GGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGGAACTGAGACCCCACCAGAAGCCTCCAGAGCTGTGGA GAATGGCTGCTGTGTGACCAAGGAAGGCCGTGGAGCCTCTGCAGGAGAGGGCAAAGAACAGAAGCCGACC AACATCGAATCCATGAAAATGGAGGGCTCCCGGGGCCGACTGCGAGGTGGCTTGGGCTGGGAGTCCAGCC TCCGTCAGCGACCCATGCCAAGACTCACCTTCCAGGCAGGGGACCCCTACTACATCAGCAAACGGAAACG GGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCTGAGAAGAAAGCCAAGGTAATTGCAGTAATGAATGCT GTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGAAGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGC CCACGGACCCTGCTTCTCCGACTGTGGCCACCACCCCTGAGCCAGTAGGAGGGGATGCTGGGGACAAGAA TGCTACCAAAGCAGCCGACGATGAGCCTGAGTATGAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTG TGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAGGCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCC GAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTTCGGAGATGGCAAGTTCTCAGTGGTGTGTGTGGAGAA GCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTCCACCAGGCCACCTACAACAAGCAGCCCATGTACCGC AAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCAGCCGTGCCGGGAAGCTGTTTCCAGCTTGCCATGACA GTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGTGCAGAACAAGCAGATGATTGAATGGGCCCTCGGTGG CTTCCAGCCCTCGGGTCCTAAGGGCCTGGAACCACCAGAAGAAGAGAAGAATCCTTACAAGGAAGTTTAC ACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTTACGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGA GCACAACAGAGAAACCTAAGGTCAAGGAGATCATTGATGAGCGCACAAGGGAGCGGCTGGTGTATGAGGT GCGCCAGAAGTGCAGAAACATCGAGGACATTTGTATCTCATGTGGGAGCCTCAATGTCACCCTGGAGCAC CCACTCTTCATTGGAGGCATGTGCCAGAACTGTAAGAACTGCTTCTTGGAGTGTGCTTACCAGTATGACG ACGATGGGTACCAGTCCTATTGCACCATCTGCTGTGGGGGGCGTGAAGTGCTCATGTGTGGGAACAACAA CTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGATCTCTTGGTGGGGCCAGGAGCTGCTCAGGCAGCCATT AAGGAAGACCCCTGGAACTGCTACATGTGCGGGCATAAGGGCACCTATGGGCTGCTGCGAAGACGGGAAG ACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAATAACCATGACCAGGAATTTGACCCCCCAAAGGTTTA CCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATCCGCGTGCTGTCTCTCTTTGATGGGATTGCTACAGGG CTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGGACCGCTACATTGCCTCCGAGGTGTGTGAGGACTCCA TCACGGTGGGCATGGTGCGGCACCAGGGAAAGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAA GCATATCCAGGAGTGGGGCCCATTCGACCTGGTGATTGGAGGCAGTCCCTGCAATGACCTCTCCATTGTC AACCCTGCCCGCAAGGGACTTTATGAGGGTACTGGCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATG ATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTTCTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGT TAGTGACAAGAGGGACATCTCGCGATTTCTTGAGTCTAACCCCGTGATGATTGACGCCAAAGAAGTGTCT GCTGCACACAGGGCCCGTTACTTCTGGGGTAACCTTCCTGGCATGAACAGGCCTTTGGCATCCACTGTGA ATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCACGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCAT TACCACCAGGTCAAACTCTATAAAGCAGGGCAAAGACCAGCATTTCCCCGTCTTCATGAACGAGAAGGAG GACATCCTGTGGTGCACTGAAATGGAAAGGGTGTTTGGCTTCCCCGTCCACTACACAGACGTCTCCAACA TGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCGATCGTGGAGCGTGCCGGTCATCCGCCACCTCTTCGC TCCGCTGAAGGAATATTTTGCTTGTGTGTAAGGGACATGGGGGCAAACTGAAGTAGTGATGATAAAAAAG TTAAACAAACAAACAAACAAAAAACAAAACAAAACAATAAAACACCAAGAACGAGAGGACGGAGAAAAGT TCAGCACCCAGAAGAGAAAAAGGAATTTAAAGCAAACCACAGAGGAGGAAAACGCCGGAGGGCTTGGCCT TGCAAAAGGGTTGGACATCATCTCCTGAGTTTTCAATGTTAACCTTCAGTCCTATCTAAAAAGCAAAATA GGCCCCTCCCCTTCTTCCCCTCCGGTCCTAGGAGGCGAACTTTTTGTTTTCTACTCTTTTTCAGAGGGGT TTTCTGTTTGTTTGGGTTTTTGTTTCTTGCTGTGACTGAAACAAGAGAGTTATTGCAGCAAAATCAGTAA CAACAAAAAGTAGTAGCCTTGGAGAGGAAAGGGAGAGAGGGAAAATTCTATAAAAACTTAAAATATTGGT TTTTTTTTTTTTCCTTTTCTATATATCTCTTTGGTTGTCTCTAGCCTGATCAGATAGGAGCACAAACAGG AAGAGAATAGAGACCCTCGGAGGCAGAGTCTCCTCTCCCACCCCCGAGCAGTCTCAACAGCACCATTCCT GGTCATGCAAAACAGAACCCAACTAGCAGCAGGGCGCTGAGAGAACACCACACCAGACACTTTTCTACAG TATTTCAGGTGCCTACCACACAGGAAACCTTGAAGAAAACCAGTTTCTAGAAGCCGCTGTTACCTCTTGT TTACAGTTTATATATATATGATAGATATGAGATATATATATATAAAAGGTACTGTTAACTACTGTACATC CCGACTTCATAATGGTGCTTTCAAAACAGCGAGATGAGCAAAGACATCAGCTTCCGCCTGGCCCTCTGTG CAAAGGGTTTCAGCCCAGGATGGGGAGAGGGGAGCAGCTGGAGGGGGTTTTAACAAACTGAAGGATGACC CATATCACCCCCCACCCCTGCCCCATGCCTAGCTTCACCTGCCAAAAAGGGGCTCAGCTGAGGTGGTCGG ACCCTGGGGAAGCTGAGTGTGGAATTTATCCAGACTCGCGTGCAATAACCTTAGAATATGAATCTAAAAT GACTGCCTCAGAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_007872
Insert Size 2727 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC007466, AAH07466
RefSeq Size 4094 bp
RefSeq ORF 2727 bp
Locus ID 13435
UniProt ID O88508
Cytogenetics 12 A1.1
Summary This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. provided by RefSeq, Nov 2012
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1, also known as Dnmt3a1). Variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Resources
"NM_007872" in other vectors (4)
SKU Description Size Price
MG211146 DNMT3A (tGFP-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 1 10 ug
$789.00 $1,031.00
MR211146 DNMT3A (Myc-DDK-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 1 10 ug
$589.00 $831.00
MR211146L3 Lenti ORF clone of DNMT3A (Myc-DDK-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 1 10 ug
$1,131.00
MR211146L4 Lenti ORF clone of DNMT3A (mGFP-tagged) - Mouse DNA methyltransferase 3A (DNMT3A), transcript variant 1 10 ug
$1,131.00

file-alt Citations

DNMT3a promotes osteoblast differentiation and alleviates osteoporosis via the PPARγ/SCD1/GLUT1 axis
Lu, Wang, Wu et al
Epigenomics (2022) 14 (12), 777-792 DOI: 10.2217/epi-2021-0391
Cited by 15
AI Summary: OriGene products, specifically the lentiviral particles pCMV6-Entry, pCMV6-PPARγ (MR208132), pCMV6-DNMT3a (MC200622), and pCMV6-SCD1 (MR225893), were used to manipulate gene expression in osteoblast cells, enabling the study of DNMT3a, PPARγ, and SCD1 roles in osteoblast differentiation and osteoporosis.
Snippet: 549V), and subsequently transduced with pCMV6-Entry, PCMV6-PPARγ (MR208132), PCMV6-DNMT3a (MC200622) or PCMV6-SCD1 (MR225893). All lentivirus particles were obtained from OriGene Technologies (MD, USA). After transduction, the cells were treated with 400 μg/ml G418 for 4 days [18] or with 5 μg/ml puromycin for 7 days [19]
Engineering of a Histone-Recognition Domain in Dnmt3a Alters the Epigenetic Landscape and Phenotypic Features of Mouse ESCs
Noh, Wang, Kim et al
Mol. Cell (2015)
Cited by 93
AI Summary: OriGene’s pCMV6-Dnmt3a plasmid (MC200622) was used as the source of the Dnmt3a2 coding sequence for generating wild-type and mutant constructs in this study.
Snippet: The coding sequence of Dnmt3a2 was amplified from pCMV6-Dnmt3a (MC200622, OriGene) and subcloned into pBluescriptKS. Plasmids encoding mutant Dnmt3a2 were made from pBluescriptKS-Dnmt3a2 by site-directed mutagenesis
Showing 1-2 of 2 papers.
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