Pfkl (NM_008826) Mouse Tagged ORF Clone
$589.00
$715.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Pfkl |
| Synonyms | AA407869; ATP-PFK; PFK-B; PFK-L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008826 |
| ORF Size | 2340 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008826.1, NM_008826.2, NM_008826.3, NM_008826.4, NP_032852.2 |
| RefSeq Size | 3741 bp |
| RefSeq ORF | 2343 bp |
| Locus ID | 18641 |
| UniProt ID | P12382 |
| Cytogenetics | 10 39.72 cM |
| MW | 85.4 kDa |
| Summary | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (PubMed:26194095).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008826" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC203910 | Pfkl (untagged) - Mouse phosphofructokinase, liver, B-type (Pfkl), (10ug) | 10 ug |
$715.00
|
|
| MG210614 | Pfkl (tGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) | 10 ug |
$915.00
|
|
| MR210614L3 | Lenti ORF clone of Pfkl (Myc-DDK-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) | 10 ug |
$1,015.00
|
|
| MR210614L4 | Lenti ORF clone of Pfkl (mGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) | 10 ug |
$1,015.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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