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Pfkl (NM_008826) Mouse Untagged Clone

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SKU
MC203910
Pfkl (untagged) - Mouse phosphofructokinase, liver, B-type (Pfkl), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $715.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pfkl
Synonyms AA407869; ATP-PFK; PFK-B; PFK-L
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC020097
CGGACGCGTGGGCGCGGAACGCAGAGCGGCGGGAGAGGAGCTTGGGCTCCTGGTCTCCGCTGCCGCCATG GCTACCGTGGACCTGGAGAAACTGCGGATGTCGGGGGCTGGCAAGGCCATTGGAGTGCTGACCAGCGGCG GTGATGCGCAAGGTATGAATGCTGCTGTCAGGGCTGTGACCCGTATGGGCATATATGTGGGGGCCAAAGT CTTCCTCATCTATGAGGGCTACGAGGGCCTTGTGGAAGGAGGCGAGAACATCAAGCCAGCCAACTGGCTC AGCGTTTCCAATATCATCCAGCTGGGTGGCACCATTATTGGCAGTGCCCGCTGTAAGGCCTTCACTACGA GGGAAGGCCGCCTGGCTGCAGCCTACAATCTGCTCCAACACGGCATCACCAACCTGTGTGTCATCGGTGG CGATGGCAGCCTCACGGGGGCCAACATCTTCCGCAACGAGTGGGGCAGCTTGCTGGAGGAGCTGGTGAAG GAAGGCAAGATCTCAGAGTCCACAGCTCAGAACTACGCACACTTGACCATCGCCGGTCTGGTGGGCTCCA TCGATAATGACTTCTGCGGCACTGACATGACCATTGGCACAGACTCAGCCCTGCACCGCATTATGGAGGT CATTGACGCCATCACTACCACTGCCCAAAGTCACCAGAGGACCTTTGTTTTGGAGGTGATGGGACGGCAC TGCGGGTACCTGGCGCTGGTGTCTGCGCTGGCTTCCGGGGCTGATTGGCTATTCATTCCTGAAGCGCCCC CTGAGGATGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGCGAGACTCGGAGCCGAGGCTCTCGGCTGAA CATCATCATCATCGCAGAGGGCGCCATTGACCGGCATGGAAAGCCTATCTCATCCAGCTACGTGAAGGAT CTGGTGGTTCAGAGGCTGGGCTTCGATACACGAGTGACTGTGCTGGGTCATGTACAGCGAGGAGGGACGC CTTCAGCCTTCGACCGAATCCTGAGTAGCAAGATGGGTATGGAGGCCGTGATGGCGCTGCTAGAGGCCAC GCCTGACACGCCGGCCTGTGTGGTCAGCCTCTCCGGGAACCAGTCTGTGAGGCTGCCTCTCATGGAGTGT GTGCAAGTGACAAAGGACGTGCAGAAGGCCATGGACGAGGAGAGGTTTGACGAGGCCATCCAGCTCCGTG GCAGGAGCTTTGAGAACAACTGGAAAATTTACAAGCTCCTTGCCCACCAGAAGGTGTCTAAAGAGAAGTC CAACTTCTCCCTGGCCATCCTGAATGTGGGGGCTCCAGCTGCTGGCATGAATGCAGCTGTGCGCTCCGCA GTGCGCACCGGTATCTCCGAGGGACACACGGTATACATCGTGCATGATGGCTTTGAGGGTCTGGCCAAGG GTCAGGTGCAAGAAGTGGGCTGGCATGATGTGGCAGGCTGGCTGGGACGTGGTGGCTCGATGCTGGGGAC CAAGAGGACACTGCCCAAGCCCCATCTGGAGGCCATTGTGGAGAATCTCCGCACCTACAACATCCACGCC CTGCTGGTGATTGGTGGCTTTGAGGCCTACGAGGGTGTGCTGCAGCTGGTGGAGGCCCGGGGGCGCTACG AGGAACTCTGCATTGTCATGTGCGTCATCCCAGCCACCATCAGCAACAATGTGCCTGGCACTGACTTCAG CCTGGGCTCAGACACGGCTGTCAACGCTGCAATGGAGAGTTGTGATCGCATCAAGCAGTCAGCCTCAGGG ACAAAGCGGCGTGTGTTCATTGTAGAGACCATGGGGGGCTACTGTGGCTACCTGGCCACTGTGACCGGCA TTGCTGTGGGTGCCGATGCTGCCTACGTCTTTGAGGACCCTTTCAACATCCATGACTTAAAGGCCAATGT GGAGCATATGACAGAGAAGATGAAGACAGACATCCAGAGGGGACTGGTGCTCCGGAACGAGAAGTGTCAC GAACACTACACCACAGAGTTCCTATACAACCTGTACTCCTCAGAAGGCAGGGGCGTGTTTGACTGCAGGA CCAATGTGCTGGGCCACTTACAGCAGGGTGGTGCTCCAACCCCTTTCGACCGGAACTATGGGACCAAACT GGGGGTGAAGGCCATGTTGTGGGTGTCTGAGAAGCTACGTGATGTCTACCGTAAAGGGCGGGTGTTTGCC AATGCTCCAGACTCAGCCTGTGTGATCGGCCTGCGGAAGAAGGTAGTGGCCTTCAGTCCGGTCACAGAAC TCAAGAAAGAGACTGATTTTGAGCACCGCATGCCCCGGGAGCAGTGGTGGCTGAATCTGCGGCTGATGCT GAAGATGCTGGCACACTATCGCATCAGCATGGCAGACTATGTGTCTGGGGAGCTGGAGCACGTCACACGC CGCACCTTGAGCATAGACAAGGGTTTCTGAGCTTACTGTCACTACTGTTCCTGCGTTCCCGGACTCTCAC CCTCCCAGTGCTAGCCACAGATCCCAGCACCGAGGGGAACTCAAGGGGAACTCGGAGCCCGGGGCTGGCT GAGGGATGTGGGGGTCTACTGTGCTGCTCTGGGGCTCTTCCCTTAGCCCTCCCCCCACCCCCAGGACAGA GAGCCTGTGGGGCCCCTACCTTCCAGTCAGACCCACATTGCCTCTCCCTCACTGGCCAGCTGGCTGCCTC ACCACACGGCTGAAAAGAGCCTCACTTTTTTAGAAATAAAGTCACCTGTCTAGAGCAAAAAAAAAAAAAA AA
Restriction Sites RsrII-NotI |
ACCN NM_008826
Insert Size 2343 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC020097, AAH20097
RefSeq Size 2732 bp
RefSeq ORF 2343 bp
Locus ID 18641
UniProt ID P12382
Cytogenetics 10 39.72 cM
Summary Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (PubMed:26194095).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008826" in other vectors (4)
SKU Description Size Price
MG210614 Pfkl (tGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) 10 ug
$915.00
MR210614 Pfkl (Myc-DDK-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) 10 ug
$589.00 $715.00
MR210614L3 Lenti ORF clone of Pfkl (Myc-DDK-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) 10 ug
$1,015.00
MR210614L4 Lenti ORF clone of Pfkl (mGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) 10 ug
$1,015.00

file-alt Citations

Nucleoredoxin regulates glucose metabolism via phosphofructokinase 1
Funato, Hayashi, Irino et al
Biochem. Biophys. Res. Commun. (2013) 440 (4), 737-42 DOI: 10.1016/j.bbrc.2013.09.138
Cited by 31
AI Summary: OriGene’s mouse PFK1 liver form cDNA (catalog number MC203910) was used as an expression construct to study the interaction between nucleoredoxin and PFK1 in this research.
Snippet: 2. Materials and Methods. 2.1. Expression constructs. Mouse PFK1 cDNAs [platelet (P), muscle (M), and liver (L) forms] were purchased from Invitrogen (IMAGE clones 2655383 and 3495173 for P and M forms, respectively) and ORIGENE (MC203910 for the L form)
Showing 1-1 of 1 papers.
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