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Satb1 (NM_009122) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210533
Satb1 (Myc-DDK-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $700.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Satb1
Synonyms 2610306G12Rik; AW413156
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210533 representing NM_009122
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTGAGTGATCCGA
AGGGTCCACCCGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCTCTAGGAAGAGGAAGGCTTGGGAG
CACAGGTGGAAAGATGCAGGGAGTGCCTTTAAAACACTCGGGCCATCTCATGAAAACCAACCTTAGGAAA
GGAACCATGTTACCAGTTTTCTGCGTGGTGGAACATTATGAAAACGCCATTGAGTATGATTGCAAGGAGG
AGCACGCGGAATTTGTATTGGTGAGAAAGGATATGCTTTTCAACCAGCTGATAGAGATGGCGTTGCTGTC
TCTAGGCTATTCACACAGCTCTGCTGCCCAAGCCAAAGGGCTCATCCAGGTTGGGAAGTGGAATCCAGTT
CCACTGTCGTATGTGACAGATGCCCCTGATGCCACGGTGGCAGACATGCTTCAAGATGTGTATCATGTGG
TCACCCTCAAAATTCAGTTACACAGTTGCCCTAAACTAGAAGACTTGCCTCCTGAACAATGGTCGCACAC
CACAGTAAGGAATGCTCTGAAGGACTTACTGAAAGATATGAACCAGAGTTCGTTGGCCAAGGAGTGCCCC
CTTTCACAGAGCATGATCTCCTCCATTGTGAACAGCACGTACTATGCAAATGTCTCAGCAGCAAAATGTC
AAGAATTTGGAAGGTGGTACAAACATTTCAAGAAGACAAAGGATATGATGGTTGAGATGGATAGTCTGTC
TGAACTATCCCAGCAAGGTGCCAACCACGTCAATTTTGGCCAGCAGCCTGTCCCAGGAAACACAGCTGAG
CAGCCTCCATCCCCTGCGCAGCTCTCTCACGGCAGTCAGCCCTCTGTCCGGACCCCTCTTCCGAACCTGC
ACCCTGGGCTTGTGTCAACACCGATCAGTCCTCAGCTGGTCAACCAACAGCTGGTGATGGCTCAGTTGCT
GAACCAGCAGTATGCAGTGAACAGACTCTTAGCCCAGCAGTCCTTAAACCAACAGTACTTGAACCACCCT
CCCCCTGTCAGTAGGTCTATGAACAAGCCTTTGGAGCAGCAAGTTTCCACAAACACGGAGGTCTCTTCTG
AAATCTACCAGTGGGTGCGGGATGAACTGAAACGAGCCGGAATCTCACAGGCAGTATTTGCACGCGTGGC
TTTTAACCGAACTCAGGGATTGCTTTCTGAAATCCTCCGAAAGGAAGAGGACCCCAAGACTGCATCCCAG
TCTCTGCTGGTAAACCTTCGGGCTATGCAGAATTTCTTACAGTTGCCGGAAGCCGAAAGAGACCGGATAT
ACCAGGATGAGAGGGAAAGGAGCTTGAACGCAGCCTCAGCCATGGGTCCTGCCCCGCTGCTGAGCACACC
ACCCAGCCGCCCTCCCCAGGTGAAAACAGCTACCCTTGCCACTGAGAGAAATGGGAAGCCAGAGAACAAT
ACTATGAACATTAATGCCTCCATTTATGATGAGATTCAGCAGGAAATGAAGCGTGCTAAAGTGTCCCAAG
CACTGTTTGCAAAGGTTGCCGCCACAAAAAGCCAGGGATGGCTGTGTGAGCTGTTGCGCTGGAAAGAAGA
TCCTTCTCCAGAAAACAGGACCCTGTGGGAGAACCTGTCGATGATCCGAAGATTCCTCAGTCTGCCTCAG
CCTGAGCGCGATGCCATCTATGAGCAGGAGAGCAATGCTGTGCATCACCATGGCGACAGACCTCCCCACA
TCATCCACGTTCCAGCAGAACAGATTCAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCA
GCCACCGCCGCCACCGCCGCAGCCACAGCCACAGCCCCAGGCAGGCCCCAGACTCCCCCCACGGCAGCCC
ACCGTGGCCTCCTCCGCGGAGTCCGATGAGGAAAACCGGCAGAAGACCAGGCCACGAACCAAAATTTCCG
TGGAAGCCCTGGGGATCCTTCAGAGTTTCATCCAAGATGTGGGCCTGTACCCAGATGAAGAGGCTATCCA
GACTCTGTCTGCACAGCTGGACCTCCCGAAGTACACCATCATCAAGTTCTTTCAGAACCAGCGGTACTAC
CTTAAGCACCATGGCAAGCTGAAGGACAACTCCGGCTTGGAGGTGGATGTGGCCGAGTACAAAGATGAGG
AGTTGCTTAAGGATTTGGAAGAGAGCGTCCAGGATAAAAACGCCAACACCCTTTTCTCAGTGAAACTAGA
GGAAGAGCTGTCGGTGGAAGGGAGCACAGACGTTAATGCCGACTTGAAAGAC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210533 representing NM_009122
Red=Cloning site Green=Tags(s)

MDHLNEATQGKEHSEMSNNVSDPKGPPAKIARLEQNGSPLGRGRLGSTGGKMQGVPLKHSGHLMKTNLRK
GTMLPVFCVVEHYENAIEYDCKEEHAEFVLVRKDMLFNQLIEMALLSLGYSHSSAAQAKGLIQVGKWNPV
PLSYVTDAPDATVADMLQDVYHVVTLKIQLHSCPKLEDLPPEQWSHTTVRNALKDLLKDMNQSSLAKECP
LSQSMISSIVNSTYYANVSAAKCQEFGRWYKHFKKTKDMMVEMDSLSELSQQGANHVNFGQQPVPGNTAE
QPPSPAQLSHGSQPSVRTPLPNLHPGLVSTPISPQLVNQQLVMAQLLNQQYAVNRLLAQQSLNQQYLNHP
PPVSRSMNKPLEQQVSTNTEVSSEIYQWVRDELKRAGISQAVFARVAFNRTQGLLSEILRKEEDPKTASQ
SLLVNLRAMQNFLQLPEAERDRIYQDERERSLNAASAMGPAPLLSTPPSRPPQVKTATLATERNGKPENN
TMNINASIYDEIQQEMKRAKVSQALFAKVAATKSQGWLCELLRWKEDPSPENRTLWENLSMIRRFLSLPQ
PERDAIYEQESNAVHHHGDRPPHIIHVPAEQIQQQQQQQQQQQQQQQPPPPPPQPQPQPQAGPRLPPRQP
TVASSAESDEENRQKTRPRTKISVEALGILQSFIQDVGLYPDEEAIQTLSAQLDLPKYTIIKFFQNQRYY
LKHHGKLKDNSGLEVDVAEYKDEELLKDLEESVQDKNANTLFSVKLEEELSVEGSTDVNADLKD

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_009122
ORF Size 2292 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009122.2, NP_033148.2
RefSeq Size 6228 bp
RefSeq ORF 2295 bp
Locus ID 20230
UniProt ID Q60611
Cytogenetics 17 C
MW 85.9 kDa
Summary Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis (By similarity). Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009122" in other vectors (4)
SKU Description Size Price
MC200989 Satb1 (untagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2, (10ug) 10 ug
$700.00
MG210533 Satb1 (tGFP-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1) 10 ug
$900.00
MR210533L3 Lenti ORF clone of Satb1 (Myc-DDK-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2 10 ug
$1,000.00
MR210533L4 Lenti ORF clone of Satb1 (mGFP-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2 10 ug
$1,000.00

Plasmid Map for MR210533

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210533
Circular map for MR210533

file-alt Citations

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