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Satb1 (NM_009122) Mouse Untagged Clone

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SKU
MC200989
Satb1 (untagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $700.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Satb1
Synonyms 2610306G12Rik; AW413156
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC011132 sequence for NM_009122
GCGGCGGGCGGCGAGGGTGCGCGGCGGGGGCGGTGCAGTTCTGGCAGGTGATCTGTAAGACAGTGACTGA GTATGGATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTGAGTGATCC GAAGGGTCCACCCGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCTCTAGGAAGAGGAAGGCTTGGG AGCACAGGTGGAAAGATGCAGGGAGTGCCTTTAAAACACTCGGGCCATCTCATGAAAACCAACCTTAGGA AAGGAACCATGTTACCAGTTTTCTGCGTGGTGGAACATTATGAAAACGCCATTGAGTATGATTGCAAGGA GGAGCACGCGGAATTTGTATTGGTGAGAAAGGATATGCTTTTCAACCAGCTGATAGAGATGGCGTTGCTG TCTCTAGGCTATTCACACAGCTCTGCTGCCCAAGCCAAAGGGCTCATCCAGGTTGGGAAGTGGAATCCAG TTCCACTGTCGTATGTGACAGATGCCCCTGATGCCACGGTGGCAGACATGCTTCAAGATGTGTATCATGT GGTCACCCTCAAAATTCAGTTACACAGTTGCCCTAAACTAGAAGACTTGCCTCCTGAACAATGGTCGCAC ACCACAGTAAGGAATGCTCTGAAGGACTTACTGAAAGATATGAACCAGAGTTCGTTGGCCAAGGAGTGCC CCCTTTCACAGAGCATGATCTCCTCCATTGTGAACAGCACGTACTATGCAAATGTCTCAGCAGCAAAATG TCAAGAATTTGGAAGGTGGTACAAACATTTCAAGAAGACAAAGGATATGATGGTTGAGATGGATAGTCTG TCTGAACTATCCCAGCAAGGTGCCAACCACGTCAATTTTGGCCAGCAGCCTGTCCCAGGAAACACAGCTG AGCAGCCTCCATCCCCTGCGCAGCTCTCTCACGGCAGTCAGCCCTCTGTCCGGACCCCTCTTCCGAACCT GCACCCTGGGCTTGTGTCAACACCGATCAGTCCTCAGCTGGTCAACCAACAGCTGGTGATGGCTCAGTTG CTGAACCAGCAGTATGCAGTGAACAGACTCTTAGCCCAGCAGTCCTTAAACCAACAGTACTTGAACCACC CTCCCCCTGTCAGTAGGTCTATGAACAAGCCTTTGGAGCAGCAAGTTTCCACGAACACGGAGGTCTCTTC TGAAATCTACCAGTGGGTGCGGGATGAACTGAAACGAGCCGGAATCTCACAGGCAGTATTTGCACGCGTG GCTTTTAACCGAACTCAGGGATTGCTTTCTGAAATCCTCCGAAAGGAAGAGGACCCCAAGACTGCATCCC AGTCTCTGCTGGTAAACCTTCGGGCTATGCAGAATTTCTTACAGTTGCCGGAAGCCGAAAGAGACCGGAT ATACCAGGATGAGAGGGAAAGGAGCTTGAACGCAGCCTCAGCCATGGGTCCTGCCCCGCTGCTGAGCACA CCACCCAGCCGCCCTCCCCAGGTGAAAACAGCTACCCTTGCCACTGAGAGAAATGGGAAGCCAGAGAACA ATACTATGAACATTAATGCCTCCATTTATGACGAGATTCAGCAGGAAATGAAGCGTGCTAAAGTGTCCCA AGCACTGTTTGCAAAGGTTGCCGCCACAAAAAGCCAGGGATGGCTGTGTGAGCTGTTGCGCTGGAAAGAA GATCCTTCTCCAGAAAACAGGACCCTGTGGGAGAACCTGTCGATGATCCGAAGATTCCTCAGTCTGCCCC AGCCTGAGCGCGATGCCATCTATGAGCAGGAGAGCAATGCTGTGCATCACCATGGCGACAGACCTCCCCA CATCATCCACGTTCCAGCAGAACAGATTCAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAG CAGCCACCGCCGCCACCGCCGCAGCCACAGCCACAGCCCCAGGCAGGCCCCAGACTCCCCCCACGGCAGC CCACCGTGGCCTCCTCCGCGGAGTCCGATGAGGAAAACCGGCAGAAGACCAGGCCACGAACCAAAATTTC CGTGGAAGCCCTGGGGATCCTTCAGAGTTTCATCCAAGATGTGGGCCTGTACCCAGATGAAGAGGCTATC CAGACTCTGTCTGCACAGCTGGACCTCCCGAAGTACACCATCATCAAGTTCTTTCAGAACCAGCGGTACT ACCTTAAGCACCATGGCAAGCTGAAGGACAACTCCGGCTTGGAGGTGGATGTGGCCGAGTACAAAGATGA GGAGTTGCTTAAGGATTTGGAAGAGAGCGTCCAGGATAAAAACGCCAACACCCTTTTCTCAGTGAAACTA GAGGAAGAGCTGTCGGTGGAAGGGAGCACAGACGTTAATGCCGACTTGAAAGACTGAGAGAACAGTATTC TTTTCAGCCACACCACCGGTATTTCCTAACAACATGAGAAGTCCACCTTGTGTTCACTCAGACAAACCTT CATTGTTTATTGTTTGGCCAATGAATCTTCGGAAACTTGCACAAACAGGAAGGAAGTTGGAAGGACAGGA CAGCCAGCACTCAAGGTTTTACTGTGTTTTACAAAACTGCTTGGCAGCCCCGGGTGAAGCGTCAAGGACT GTTTGGTAGAATTTGTGTTCAAGGGCTGCACCCAGGTGTTGTACCCTGTCAGCATGATACCAGAAATTGG TTTTCCTTTTGATTATTATTATTCTGGAGCCTCAAATAAGCATTATAACTTCTGTGGTTATGATTGCCTT TTCTTTAGTAATTCTTCTGTAACCCACCACACTAGTCTTTGGAAACTGGCCCCTTATTTTAAAGAGAAAA AAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_009122
Insert Size 2295 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC011132, AAH11132
RefSeq Size 2813 bp
RefSeq ORF 2295 bp
Locus ID 20230
UniProt ID Q60611
Cytogenetics 17 C
Summary Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis (By similarity). Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein.
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Documents
Resources
"NM_009122" in other vectors (4)
SKU Description Size Price
MG210533 Satb1 (tGFP-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1) 10 ug
$900.00
MR210533 Satb1 (Myc-DDK-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2 10 ug
$589.00 $700.00
MR210533L3 Lenti ORF clone of Satb1 (Myc-DDK-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2 10 ug
$1,000.00
MR210533L4 Lenti ORF clone of Satb1 (mGFP-tagged) - Mouse special AT-rich sequence binding protein 1 (Satb1), transcript variant 2 10 ug
$1,000.00

file-alt Citations

ZBTB32 is an early repressor of the CIITA and MHC class II gene expression during B cell differentiation to plasma cells
Yoon, Scharer, Majumder et al
J. Immunol. (2012) 189 (5), 2393-403 DOI: 10.4049/jimmunol.1103371
Cited by 85
AI Summary: OriGene products contributed to this study by providing expression plasmids for CBX2 (MC204062), HMGN3 (SC110044), and Rad54l (MC205395), as well as pLKO.1 lentiviral constructs containing shRNA targeting ZBTB32, which were used to investigate the regulatory role of ZBTB32 in CIITA and MHC class II gene expression during B cell differentiation.
Snippet: Expression plasmids used in transient transfection experiments, purchased from OriGene Technologies, included those for CBX2 (MC204062), HMGN3 (SC110044), Rad54l (MC205395 ... Lentivirus constructs (pLKO.1) harboring short hairpin RNA (shRNA) to specific ZBTB32 (cat
Showing 1-1 of 1 papers.
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