OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Impg1 (NM_022016) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR210410
Impg1 (Myc-DDK-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $732.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Impg1 or NM_022016"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Impg1
Synonyms A930015H12Rik; IMP150; SPACR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210410 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTTGCAAATTAAACATGCTATCTTTGTTTTGGGGATTTTTCTCCAAGTTCAAGGAATCAAAGATA
CCTCTATTAAAATATTCAGTTCTGAAATTAAAAACATAGACAAAACCCCAAGAATCGAAACAATTGAAAG
TACTTCAACAGTGCACAAAGTGTCAACCATGAAACGAATATTCGATTTGCCAAAGCTTCGAACCAAAAGA
TCAGCACTTTTCCCAGCTGCTAACATCTGTCCACAGGAATCCTTGAGACAGATTTTAGCAAGTCTTCAAG
AATATTATAGACTGAGAGTATGTCAAGAAGTCGTGTGGGAAGCATATCGTATCTTTCTGGACCGAATTCC
TGACACAGAGGAATATCAAGACTGGGTCAGCCTCTGCCAGAAAGAAACCTTCTGCCTCTTTGACATTGGG
AAAAACTTCAGCAACTCCCAGGAGCACCTAGATCTTCTTCAGCAGAGAATAAAACAGAGAAGCTTCCCTG
GGAGGAAAGATGAGACAGCCTCCATGGAGACACTGGAAGCACCTACTGAAGCCCCTGTGGTACCCACAGA
TGTTTCCAGGATGTCCCTGGGGCCCTTCCCACTTCCTTCTGATGACACAGACCTCAAGGAGATTCTCAGT
GTCACCCTCAAGGACATTCAAAAGCCCACAACAGAAAGTAAAACAGAACCTATTCATGTGTCTGAATTCT
CATCAGAGGAGAAGGTGGAATTCAGCATCTCTCTGCCAAACCACAGGTTCAAGGCAGAGCTCACCAACTC
TGGGTCACCATACTACCAGGAACTGGTGGGACAGTCCCAACTGCAGTTGCAAAAGATATTTAAGAAACTT
CCAGGATTCGGAGAAATCCGTGTATTAGGATTTAGACCAAAGAAAGAAGAAGATGGTTCAAGCTCCACAG
AAATACAGCTTATGGCCATCTTTAAGAGGGACCATGCAGAAGCAAAAAGCCCTGATAGTCATCTACTGTC
TCTTGATTCCAACAAAATTGAAAGTGAAAGAATCCATCATGGAGTCATAGAAGACAAACAACCAGAAACC
TACCTCACAGCTACAGACCTCAAAAAACTCATCATACAACTACTAGATGGAGACCTGTCCTTGGTAGAAG
GGAAAATTCCATTCGGTGATGAAGTTACTGGGACACTCTTCAGACCTGTCACTGAACCAGATCTGCCCAA
GCCCCTTGCTGATGTCACAGAGGATGCCACTTTGAGTCCAGAACTTCCTTTCGTTGAGCCTAGGCTTGAG
GCAGTGGACAGAGAAGGATCTGAGCTGCCTGGAATGTCCTCCAAAGACAGTTCTTGGTCTCCACCTGTAT
CAGCCTCAATTTCCCGATCAGAAAATCTACCTTCGTTTACACCTAGCATCTTCTCTCTAGATGCTCAAAG
CCCCCCTCCCTTGATGACCACTGGCCCAACAGCACTCATCCCCAAGCCCACTCTCCCCACTATCGATTAT
TCTACCATCCGCCAATTGCCTCTGGAATCGTCACATTGGCCTGCATCCTCCAGTGACAGAGAGCTGATCA
CAAGCAGCCATGACACAATCCGAGACCTAGATGGCATGGATGTGTCTGACACGCCAGCCTTGTCAGAAAT
ATCAGAACTGAGTGGATACGATTCTGCCTCGGGTCAGTTCTTGGAGATGACCACACCCATCCCAACAGTA
CGGTTCATCACCACCAGCTCCGAGACCATTGCCACCAAGGGCCAGGAGCTAGTGGTATTCTTCAGCCTGC
GTGTTGCTAACATGCCGTTCTCCTATGACCTGTTCAACAAGAGTTCTCTGGAGTATCAAGCCCTGGAACA
ACGATTCACAGACCTGCTGGTTCCCTATCTACGATCGAATCTTACGGGATTTAAGCAACTGGAAATACTC
AGCTTCAGAAACGGAAGTGTGATCGTGAACAGCAAAGTGCGGTTTGCAAAGGCGGTACCCTACAACCTCA
CCCAGGCCGTGCGCGGGGTCTTGGAGGATCTTCGGTCCACCGCAGCTCAAGGGCTCAATCTGGAAATCGA
AAGCTACTCCCTCGACATTGAACCAGCTGATCAGGCGGATCCCTGCAAACTCCTAGACTGTGGCAAATTT
GCCCAGTGTGTAAAGAATGAGTGGACAGAGGAAGCAGAGTGTCGCTGCAGACAGGGACATGAGAGCCACG
GGACCCTGGACTACCAGACCCTGAACCTCTGTCCCCCTGGAAAGACTTGTGTGGCCGGCCGAGAACAAGC
AACTCCATGCAGGCCACCAGATCACTCTACAAACCAAGCTCAGGAACCTGGTGTTAAAAAGCTACGTCAG
CAAAATAAGGTAGTCAAGAAAAGAAATTCTAAACTATCAGCTATAGGATTTGAAGAATTTGAAGACCAGG
ACTGGGAGGGAAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210410 protein sequence
Red=Cloning site Green=Tags(s)

MNLQIKHAIFVLGIFLQVQGIKDTSIKIFSSEIKNIDKTPRIETIESTSTVHKVSTMKRIFDLPKLRTKR
SALFPAANICPQESLRQILASLQEYYRLRVCQEVVWEAYRIFLDRIPDTEEYQDWVSLCQKETFCLFDIG
KNFSNSQEHLDLLQQRIKQRSFPGRKDETASMETLEAPTEAPVVPTDVSRMSLGPFPLPSDDTDLKEILS
VTLKDIQKPTTESKTEPIHVSEFSSEEKVEFSISLPNHRFKAELTNSGSPYYQELVGQSQLQLQKIFKKL
PGFGEIRVLGFRPKKEEDGSSSTEIQLMAIFKRDHAEAKSPDSHLLSLDSNKIESERIHHGVIEDKQPET
YLTATDLKKLIIQLLDGDLSLVEGKIPFGDEVTGTLFRPVTEPDLPKPLADVTEDATLSPELPFVEPRLE
AVDREGSELPGMSSKDSSWSPPVSASISRSENLPSFTPSIFSLDAQSPPPLMTTGPTALIPKPTLPTIDY
STIRQLPLESSHWPASSSDRELITSSHDTIRDLDGMDVSDTPALSEISELSGYDSASGQFLEMTTPIPTV
RFITTSSETIATKGQELVVFFSLRVANMPFSYDLFNKSSLEYQALEQRFTDLLVPYLRSNLTGFKQLEIL
SFRNGSVIVNSKVRFAKAVPYNLTQAVRGVLEDLRSTAAQGLNLEIESYSLDIEPADQADPCKLLDCGKF
AQCVKNEWTEEAECRCRQGHESHGTLDYQTLNLCPPGKTCVAGREQATPCRPPDHSTNQAQEPGVKKLRQ
QNKVVKKRNSKLSAIGFEEFEDQDWEGN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022016
ORF Size 2394 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022016.3, NP_071299.3
RefSeq Size 3587 bp
RefSeq ORF 2397 bp
Locus ID 63859
UniProt ID Q8R1W8
Cytogenetics 9 43.99 cM
MW 89.5 kDa
Summary May interact with hyaluronan which may serve to form a basic macromolecular scaffold comprising the insoluble interphotoreceptor matrix.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_022016" in other vectors (4)
SKU Description Size Price
MC221682 Impg1 (untagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1), (10ug) 10 ug
$805.00
MG210410 Impg1 (tGFP-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1) 10 ug
$932.00
MR210410L3 Lenti ORF clone of Impg1 (Myc-DDK-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1) 10 ug
$1,032.00
MR210410L4 Lenti ORF clone of Impg1 (mGFP-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1) 10 ug
$1,032.00

Plasmid Map for MR210410

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210410
Circular map for MR210410

file-alt Citations

Interphotoreceptor matrix proteoglycans IMPG1 and IMPG2 proteolyze in the SEA domain and reveal localization mutual dependency
Mitchell, Coulter, Geldenhuys et al
Sci Rep (2022) 12 (1), 15535 DOI: 10.1038/s41598-022-19910-1
Cited by 18
AI Summary: OriGene contributed to this study by providing a human IMPG1 plasmid with a C-terminal DDK tag (MR210410 vector), which enabled the researchers to express and analyze IMPG1 proteolysis and the effects of disease-associated mutations.
Snippet: … Plasmid design and protein expression Human IMPG1 with a DDK tag in the C-terminus, under the CMV promotor in an MR210410 vector, was generated by Origene. The second set of plasmids contained three versions of mouse IMPG1: one WT, and two with point mutations in L583P or L630F. … Full-length mouse IMPG1 var;Human IMPG1 with a DDK tag in the C-terminus, under the CMV promotor in an MR210410 vector, was generated by Origene. The second set of plasmids contained three versions of mouse IMPG1: one WT, and two with point mutations in L583P or L630F. …
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products