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Impg1 (NM_022016) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210410
Impg1 (tGFP-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $932.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Impg1
Synonyms A930015H12Rik; IMP150; SPACR
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210410 representing NM_022016
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTTGCAAATTAAACATGCTATCTTTGTTTTGGGGATTTTTCTCCAAGTTCAAGGAATCAAAGATA
CCTCTATTAAAATATTCAGTTCTGAAATTAAAAACATAGACAAAACCCCAAGAATCGAAACAATTGAAAG
TACTTCAACAGTGCACAAAGTGTCAACCATGAAACGAATATTCGATTTGCCAAAGCTTCGAACCAAAAGA
TCAGCACTTTTCCCAGCTGCTAACATCTGTCCACAGGAATCCTTGAGACAGATTTTAGCAAGTCTTCAAG
AATATTATAGACTGAGAGTATGTCAAGAAGTCGTGTGGGAAGCATATCGTATCTTTCTGGACCGAATTCC
TGACACAGAGGAATATCAAGACTGGGTCAGCCTCTGCCAGAAAGAAACCTTCTGCCTCTTTGACATTGGG
AAAAACTTCAGCAACTCCCAGGAGCACCTAGATCTTCTTCAGCAGAGAATAAAACAGAGAAGCTTCCCTG
GGAGGAAAGATGAGACAGCCTCCATGGAGACACTGGAAGCACCTACTGAAGCCCCTGTGGTACCCACAGA
TGTTTCCAGGATGTCCCTGGGGCCCTTCCCACTTCCTTCTGATGACACAGACCTCAAGGAGATTCTCAGT
GTCACCCTCAAGGACATTCAAAAGCCCACAACAGAAAGTAAAACAGAACCTATTCATGTGTCTGAATTCT
CATCAGAGGAGAAGGTGGAATTCAGCATCTCTCTGCCAAACCACAGGTTCAAGGCAGAGCTCACCAACTC
TGGGTCACCATACTACCAGGAACTGGTGGGACAGTCCCAACTGCAGTTGCAAAAGATATTTAAGAAACTT
CCAGGATTCGGAGAAATCCGTGTATTAGGATTTAGACCAAAGAAAGAAGAAGATGGTTCAAGCTCCACAG
AAATACAGCTTATGGCCATCTTTAAGAGGGACCATGCAGAAGCAAAAAGCCCTGATAGTCATCTACTGTC
TCTTGATTCCAACAAAATTGAAAGTGAAAGAATCCATCATGGAGTCATAGAAGACAAACAACCAGAAACC
TACCTCACAGCTACAGACCTCAAAAAACTCATCATACAACTACTAGATGGAGACCTGTCCTTGGTAGAAG
GGAAAATTCCATTCGGTGATGAAGTTACTGGGACACTCTTCAGACCTGTCACTGAACCAGATCTGCCCAA
GCCCCTTGCTGATGTCACAGAGGATGCCACTTTGAGTCCAGAACTTCCTTTCGTTGAGCCTAGGCTTGAG
GCAGTGGACAGAGAAGGATCTGAGCTGCCTGGAATGTCCTCCAAAGACAGTTCTTGGTCTCCACCTGTAT
CAGCCTCAATTTCCCGATCAGAAAATCTACCTTCGTTTACACCTAGCATCTTCTCTCTAGATGCTCAAAG
CCCCCCTCCCTTGATGACCACTGGCCCAACAGCACTCATCCCCAAGCCCACTCTCCCCACTATCGATTAT
TCTACCATCCGCCAATTGCCTCTGGAATCGTCACATTGGCCTGCATCCTCCAGTGACAGAGAGCTGATCA
CAAGCAGCCATGACACAATCCGAGACCTAGATGGCATGGATGTGTCTGACACGCCAGCCTTGTCAGAAAT
ATCAGAACTGAGTGGATACGATTCTGCCTCGGGTCAGTTCTTGGAGATGACCACACCCATCCCAACAGTA
CGGTTCATCACCACCAGCTCCGAGACCATTGCCACCAAGGGCCAGGAGCTAGTGGTATTCTTCAGCCTGC
GTGTTGCTAACATGCCGTTCTCCTATGACCTGTTCAACAAGAGTTCTCTGGAGTATCAAGCCCTGGAACA
ACGATTCACAGACCTGCTGGTTCCCTATCTACGATCGAATCTTACGGGATTTAAGCAACTGGAAATACTC
AGCTTCAGAAACGGAAGTGTGATCGTGAACAGCAAAGTGCGGTTTGCAAAGGCGGTACCCTACAACCTCA
CCCAGGCCGTGCGCGGGGTCTTGGAGGATCTTCGGTCCACCGCAGCTCAAGGGCTCAATCTGGAAATCGA
AAGCTACTCCCTCGACATTGAACCAGCTGATCAGGCGGATCCCTGCAAACTCCTAGACTGTGGCAAATTT
GCCCAGTGTGTAAAGAATGAGTGGACAGAGGAAGCAGAGTGTCGCTGCAGACAGGGACATGAGAGCCACG
GGACCCTGGACTACCAGACCCTGAACCTCTGTCCCCCTGGAAAGACTTGTGTGGCCGGCCGAGAACAAGC
AACTCCATGCAGGCCACCAGATCACTCTACAAACCAAGCTCAGGAACCTGGTGTTAAAAAGCTACGTCAG
CAAAATAAGGTAGTCAAGAAAAGAAATTCTAAACTATCAGCTATAGGATTTGAAGAATTTGAAGACCAGG
ACTGGGAGGGAAAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210410 representing NM_022016
Red=Cloning site Green=Tags(s)

MNLQIKHAIFVLGIFLQVQGIKDTSIKIFSSEIKNIDKTPRIETIESTSTVHKVSTMKRIFDLPKLRTKR
SALFPAANICPQESLRQILASLQEYYRLRVCQEVVWEAYRIFLDRIPDTEEYQDWVSLCQKETFCLFDIG
KNFSNSQEHLDLLQQRIKQRSFPGRKDETASMETLEAPTEAPVVPTDVSRMSLGPFPLPSDDTDLKEILS
VTLKDIQKPTTESKTEPIHVSEFSSEEKVEFSISLPNHRFKAELTNSGSPYYQELVGQSQLQLQKIFKKL
PGFGEIRVLGFRPKKEEDGSSSTEIQLMAIFKRDHAEAKSPDSHLLSLDSNKIESERIHHGVIEDKQPET
YLTATDLKKLIIQLLDGDLSLVEGKIPFGDEVTGTLFRPVTEPDLPKPLADVTEDATLSPELPFVEPRLE
AVDREGSELPGMSSKDSSWSPPVSASISRSENLPSFTPSIFSLDAQSPPPLMTTGPTALIPKPTLPTIDY
STIRQLPLESSHWPASSSDRELITSSHDTIRDLDGMDVSDTPALSEISELSGYDSASGQFLEMTTPIPTV
RFITTSSETIATKGQELVVFFSLRVANMPFSYDLFNKSSLEYQALEQRFTDLLVPYLRSNLTGFKQLEIL
SFRNGSVIVNSKVRFAKAVPYNLTQAVRGVLEDLRSTAAQGLNLEIESYSLDIEPADQADPCKLLDCGKF
AQCVKNEWTEEAECRCRQGHESHGTLDYQTLNLCPPGKTCVAGREQATPCRPPDHSTNQAQEPGVKKLRQ
QNKVVKKRNSKLSAIGFEEFEDQDWEGN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022016
ORF Size 2394 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022016.3, NP_071299.3
RefSeq Size 3587 bp
RefSeq ORF 2397 bp
Locus ID 63859
UniProt ID Q8R1W8
Cytogenetics 9 43.99 cM
Summary May interact with hyaluronan which may serve to form a basic macromolecular scaffold comprising the insoluble interphotoreceptor matrix.UniProtKB/Swiss-Prot Function
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"NM_022016" in other vectors (4)
SKU Description Size Price
MC221682 Impg1 (untagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1), (10ug) 10 ug
$805.00
MR210410 Impg1 (Myc-DDK-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1) 10 ug
$589.00 $732.00
MR210410L3 Lenti ORF clone of Impg1 (Myc-DDK-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1) 10 ug
$1,032.00
MR210410L4 Lenti ORF clone of Impg1 (mGFP-tagged) - Mouse interphotoreceptor matrix proteoglycan 1 (Impg1) 10 ug
$1,032.00

Plasmid Map for MG210410

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210410
Circular map for MG210410

file-alt Citations

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