Ercc2 (BC034517) Mouse Tagged ORF Clone

SKU MR210391

Ercc2 (Myc-DDK-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 2 (cDNA clone MGC:27532

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ercc2
• Synonyms: AA407812; AU020867; AW240756; CXPD; Ercc-2; Mhdarco15; RCO015; XPD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210391 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGCTCAACGTGGACGGGCTGCTGGTCTACTTCCCGTACGACTACATCTACCCGGAGCAGTTCTCCT; ACATGCTGGAGCTCAAGCGCACGCTGGACGCCAAGGGTCATGGAGTCCTGGAGATGCCCTCGGGCACTGG; GAAGACAGTGTCCCTATTGGCCCTGATTGTGGCCTATCAGCGGGTCATAGAAGAGCTTCGTAAGCTACTC; AGCTTCTACGAGCAGCAGGAGGGCGAGAAGCTGCCGTTCTTAGGACTGGCTCTGAGCTCAAGAAAGAACC; TGTGCATTCATCCCGAGGTGACTCCACTGCGCTTTGGGAAGGATGTTGATGGGAAGTGTCACAGCCTAAC; GGCGTCGTATGTGCGGGCACAGTACCAGCAGGATGCCAGCCTGCCTCACTGCCGCTTCTATGAGGAATTT; GACATCCATGGACGCCAGATGCCGCTCCCTGCGGGCATCTACAACCTGGATGACCTGAAGGCCCTAGGGC; ATCGCCAGGGCTGGTGCCCCTACTTCCTGGCTCGATACTCGATCCTGCATGCCAACGTGGTGGTTTACAG; CTACCACTACCTCCTGGACCCCAAGATCGCAGACCTGGTATCCAAAGAGCTGGCTCGCAAGGCTGTTGTG; GTCTTCGATGAAGCTCACAACATCGACAATGTCTGCATTGACTCCATGAGTGTCAACCTCACCCGCAGGA; CTCTGGACCGTTGCCAGAGCAACTTAGACACCCTACAGAAGACCGTGCTCAGGATCAAGGAGACGGATGA; GCAGCGGCTGCGGGATGAGTACCGGCGCCTGGTGGAGGGCCTGCGGGAGGCCAGTGTGGCCCGGGAGACA; GATGCCCACCTGGCCAACCCTGTGCTGCCGGACGAGGTGCTGCAGGAGGCTGTGCCTGGCTCCATCCGTA; CGGCTGAGCACTTCCTGGGCTTTCTGCGGCGGCTGCTGGAGTATGTCAAGTGGCGTCTGCGCGTGCAGCA; TGTGGTGCAGGAGAGTCCACCTGCCTTTCTGAGCGGCCTGGCCCAGCGGGTGTGCATCCAGCGCAAGCCC; CTCAGGTTCTGTGCTGAACGCCTGCGCTCCCTGCTGCACACCCTGGAGATTGCCGACCTGGCCGACTTCT; CCCCGCTCACACTCCTTGCTAACTTCGCCACTCTCGTCAGCACTTACGCCAAGGGCTTCACCATTATCAT; TGAGCCCTTTGACGACAGGACCCCCACCATCGCCAACCCCGTTCTGCACTTCAGCTGTATGGACGCCTCC; TTGGCCATCAAGCCTGTGTTTGAGCGCTTCCAGTCTGTCATCATCACTTCTGGGACACTGTCCCCACTGG; ACATCTACCCCAAGATCCTGGACTTCCACCCTGTCACAATGGCAACCTTCACCATGACGCTGGCCCGAGT; CTGCCTCTGCCCGATGATCATTGGCCGTGGTAATGACCAGGTAGCAATCAGCTCCAAATTTGAGACCAGA; GAAGATATTGCTGTGATCCGAAACTATGGCAACCTCCTGCTGGAGATGTCCGCCGTGGTCCCAGATGGCA; TTGTGGCCTTCTTTACCAGCTACCAGTACATGGAAAGCACCGTGGCCTCCTGGTATGAGCAGGGCATCCT; TGAGAACATCCAGAGGAACAAACTGCTCTTCATTGAGACCCAGGATGGGGCTGAGACCAGTGTGGCCCTG; GAGAAGTACCAAGAGGCATGCGAGAATGGCCGTGGGGCCATTCTGCTCTCAGTGGCTCGGGGCAAAGTAT; CAGAAGGGATTGACTTTGTACACCACTACGGACGGGCTGTGATCATGTTTGGAGTTCCCTATGTCTATAC; CCAGAGCCGAATTCTCAAGGCCCGGCTAGAGTATCTGCGGGACCAGTTCCAGATCCGAGAGAACGACTTC; CTCACCTTTGATGCTATGCGCCATGCAGCCCAGTGTGTGGGTCGTGCCATCAGGGGCAAGACGGACTATG; GACTCATGGTCTTTGCTGACAAGCGGTTTGCTCGGGCGGACAAGCGTGGTAAGCTGCCTCGCTGGATCCA; GGAGCACCTGACCGACTCCAACCTCAACCTGACCGTGGATGAGGGTGTACAGGTCGCCAAGTACTTCCTG; CGGCAGATGGCGCAGCCCTTCCACCGGGAGGATCAGCTGGGCCTGTCGCTGCTCAGCCTGGAGCAGCTGC; AGTCAGAGGAGACACTACAGCGAATTGAGCAGATCGCACAGCAGCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210391 protein sequence; Red=Cloning site Green=Tags(s); ; MKLNVDGLLVYFPYDYIYPEQFSYMLELKRTLDAKGHGVLEMPSGTGKTVSLLALIVAYQRVIEELRKLL; SFYEQQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQQDASLPHCRFYEEF; DIHGRQMPLPAGIYNLDDLKALGHRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVV; VFDEAHNIDNVCIDSMSVNLTRRTLDRCQSNLDTLQKTVLRIKETDEQRLRDEYRRLVEGLREASVARET; DAHLANPVLPDEVLQEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESPPAFLSGLAQRVCIQRKP; LRFCAERLRSLLHTLEIADLADFSPLTLLANFATLVSTYAKGFTIIIEPFDDRTPTIANPVLHFSCMDAS; LAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMATFTMTLARVCLCPMIIGRGNDQVAISSKFETR; EDIAVIRNYGNLLLEMSAVVPDGIVAFFTSYQYMESTVASWYEQGILENIQRNKLLFIETQDGAETSVAL; EKYQEACENGRGAILLSVARGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDF; LTFDAMRHAAQCVGRAIRGKTDYGLMVFADKRFARADKRGKLPRWIQEHLTDSNLNLTVDEGVQVAKYFL; RQMAQPFHREDQLGLSLLSLEQLQSEETLQRIEQIAQQL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC034517
• ORF Size: 2217 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC034517, AAH34517
• RefSeq Size: 3500 bp
• RefSeq ORF: 2219 bp
• Locus ID: 13871
• Cytogenetics: 7 9.62 cM
• MW: 84.4 kDa
• Summary: ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.[UniProtKB/Swiss-Prot Function]