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Ercc2 (BC034517) Mouse Untagged Clone

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SKU
MC220718
Ercc2 (untagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 2 (cDNA clone MGC:27532, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $745.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ercc2
Synonyms AA407812; AU020867; AW240756; CXPD; Ercc-2; Mhdarco15; RCO015; XPD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC034517
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCTCAACGTGGACGGGCTGCTGGTCTACTTCCCGTACGACTACATCTACCCGGAGCAGTTCTCCT
ACATGCTGGAGCTCAAGCGCACGCTGGACGCCAAGGGTCATGGAGTCCTGGAGATGCCCTCGGGCACTGG
GAAGACAGTGTCCCTATTGGCCCTGATTGTGGCCTATCAGCGGGTCATAGAAGAGCTTCGTAAGCTACTC
AGCTTCTACGAGCAGCAGGAGGGCGAGAAGCTGCCGTTCTTAGGACTGGCTCTGAGCTCAAGAAAGAACC
TGTGCATTCATCCCGAGGTGACTCCACTGCGCTTTGGGAAGGATGTTGATGGGAAGTGTCACAGCCTAAC
GGCGTCGTATGTGCGGGCACAGTACCAGCAGGATGCCAGCCTGCCTCACTGCCGCTTCTATGAGGAATTT
GACATCCATGGACGCCAGATGCCGCTCCCTGCGGGCATCTACAACCTGGATGACCTGAAGGCCCTAGGGC
ATCGCCAGGGCTGGTGCCCCTACTTCCTGGCTCGATACTCGATCCTGCATGCCAACGTGGTGGTTTACAG
CTACCACTACCTCCTGGACCCCAAGATCGCAGACCTGGTATCCAAAGAGCTGGCTCGCAAGGCTGTTGTG
GTCTTCGATGAAGCTCACAACATCGACAATGTCTGCATTGACTCCATGAGTGTCAACCTCACCCGCAGGA
CTCTGGACCGTTGCCAGAGCAACTTAGACACCCTACAGAAGACCGTGCTCAGGATCAAGGAGACGGATGA
GCAGCGGCTGCGGGATGAGTACCGGCGCCTGGTGGAGGGCCTGCGGGAGGCCAGTGTGGCCCGGGAGACA
GATGCCCACCTGGCCAACCCTGTGCTGCCGGACGAGGTGCTGCAGGAGGCTGTGCCTGGCTCCATCCGTA
CGGCTGAGCACTTCCTGGGCTTTCTGCGGCGGCTGCTGGAGTATGTCAAGTGGCGTCTGCGCGTGCAGCA
TGTGGTGCAGGAGAGTCCACCTGCCTTTCTGAGCGGCCTGGCCCAGCGGGTGTGCATCCAGCGCAAGCCC
CTCAGGTTCTGTGCTGAACGCCTGCGCTCCCTGCTGCACACCCTGGAGATTGCCGACCTGGCCGACTTCT
CCCCGCTCACACTCCTTGCTAACTTCGCCACTCTCGTCAGCACTTACGCCAAGGGCTTCACCATTATCAT
TGAGCCCTTTGACGACAGGACCCCCACCATCGCCAACCCCGTTCTGCACTTCAGCTGTATGGACGCCTCC
TTGGCCATCAAGCCTGTGTTTGAGCGCTTCCAGTCTGTCATCATCACTTCTGGGACACTGTCCCCACTGG
ACATCTACCCCAAGATCCTGGACTTCCACCCTGTCACAATGGCAACCTTCACCATGACGCTGGCCCGAGT
CTGCCTCTGCCCGATGATCATTGGCCGTGGTAATGACCAGGTAGCAATCAGCTCCAAATTTGAGACCAGA
GAAGATATTGCTGTGATCCGAAACTATGGCAACCTCCTGCTGGAGATGTCCGCCGTGGTCCCAGATGGCA
TTGTGGCCTTCTTTACCAGCTACCAGTACATGGAAAGCACCGTGGCCTCCTGGTATGAGCAGGGCATCCT
TGAGAACATCCAGAGGAACAAACTGCTCTTCATTGAGACCCAGGATGGGGCTGAGACCAGTGTGGCCCTG
GAGAAGTACCAAGAGGCATGCGAGAATGGCCGTGGGGCCATTCTGCTCTCAGTGGCTCGGGGCAAAGTAT
CAGAAGGGATTGACTTTGTACACCACTACGGACGGGCTGTGATCATGTTTGGAGTTCCCTATGTCTATAC
CCAGAGCCGAATTCTCAAGGCCCGGCTAGAGTATCTGCGGGACCAGTTCCAGATCCGAGAGAACGACTTC
CTCACCTTTGATGCTATGCGCCATGCAGCCCAGTGTGTGGGTCGTGCCATCAGGGGCAAGACGGACTATG
GACTCATGGTCTTTGCTGACAAGCGGTTTGCTCGGGCGGACAAGCGTGGTAAGCTGCCTCGCTGGATCCA
GGAGCACCTGACCGACTCCAACCTCAACCTGACCGTGGATGAGGGTGTACAGGTCGCCAAGTACTTCCTG
CGGCAGATGGCGCAGCCCTTCCACCGGGAGGATCAGCTGGGCCTGTCGCTGCTCAGCCTGGAGCAGCTGC
AGTCAGAGGAGACACTACAGCGAATTGAGCAGATCGCACAGCAGCTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC034517
Insert Size 2220 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC034517, AAH34517
RefSeq Size 3500 bp
RefSeq ORF 2219 bp
Locus ID 13871
Cytogenetics 7 9.62 cM
Summary ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC034517" in other vectors (4)
SKU Description Size Price
MG210391 Ercc2 (tGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 2 (cDNA clone MGC:27532 10 ug
$877.00
MR210391 Ercc2 (Myc-DDK-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 2 (cDNA clone MGC:27532 10 ug
$589.00 $677.00
MR210391L3 Lenti ORF clone of Ercc2 (Myc-DDK-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 2 (cDNA clone MGC:27532 10 ug
$977.00
MR210391L4 Lenti ORF clone of Ercc2 (mGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 2 (cDNA clone MGC:27532 10 ug
$977.00

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