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Mthfd1l (BC030437) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210353
Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $671.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mthfd1l
Synonyms 2410004L15Rik; AI647056; Fthfsdc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210353 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCCAGACAAGCTTCGAGAGGCAGACATTGTGGTCT
TAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGCGTGGATTCCATCAGGAACCACCATTCTCAATTGCTT
CCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTCCTGGGGTCCCCGTGGACAAACTCATTGCAGAA
GAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCAGAACATGGTGAGCAGCGGCAGGAGGTGGCTCA
GAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTGAAACTGCAGCCTCTCTCACCTGTGCCCAGTGA
TATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGGATGTCCTTGCCAAGGAGATAGGATTGCTTGCA
GATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGTCCAATTGTCCCTGCTGGAGAGGTTAAAGGATC
AAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACGCCCACCCCGCTCGGAGAAGGGAAGAGTACAGT
CACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGAAAGTCAACTCCTTCGCCTGTCTGAGGCAGCCT
TCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGCAGGAGGTGGATATGCTCAGGTCATCCCCATGG
AGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCCATCACTGCCGCTAATAACTTGCTGGCCGCAGC
CATCGACACGAGAATTTTACATGAGAGCACTCAAACAGACAAGGCTCTCTACAATCGACTGGTTCCCTTA
GTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTCTCGGCTGAAGAAACTGGGGATACATAAGACTG
ATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTCGCCCGCCTCAACATTGACCCCGCAACCATCAC
GTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGAGAAAAATAACCATTGGACAGGGAAGTACAGAG
AAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGTGGCCAGCGAGATCATGGCCGTGCTGGCCTTGA
CTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGAATGGTGGTGGCCAGTGACAAAGACGGGCAGCC
TGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGACAGTTTTGATGAAAGACGCCATCAAGCCAAAT
CTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCATGCTGGTCCCTTTGCCAACATCGCTCACGGCA
ACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTGGTCGGCGAAGAAGGGTTTGTGGTGACTGAAGC
TGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCAATATCAAATGCCGAGCTTCTGGCCTGGTGCCC
AACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAAGATGCATGGAGGCGGGCCGAGTGTAACTGCTG
GGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATTCAGCTGGTAGCTGATGGCTGCTGCAACCTACA
GAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGGTGGTGGTGGCACTGAATGTCTTCAAGACGGAC
ACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAAACGGGCCGGTGCCTTTGATGCGGTCCCCTGCT
ATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTGGCCCGGGCTGTGAGAGAAGCTGCGAATAAAAG
AAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCATCGTGGAAAAGATAAGAGTCATTGCCCAGACT
GTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGCGCAGTCCAAAATCGATCGCTATACGCAACAGG
GTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCACCTCTCTCTCTCTCATGAACCTGACAAAAAGGG
AGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCCGGGCCAGCATCGGGGCTGGGTTCATTTATCCT
TTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCACCCGACCCTGCTTTTATGATATAGACCTTGACA
CAGAAACGGAGCAAGTTAAAGGCTTGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210353 protein sequence
Red=Cloning site Green=Tags(s)

MTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAAWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAE
ESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLA
DEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQP
SQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPL
VNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTE
KGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPN
LMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVP
NVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTD
TRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFQFLYDVQLPIVEKIRVIAQT
VYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYP
LVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC030437
ORF Size 2199 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC030437, AAH30437
RefSeq Size 3061 bp
RefSeq ORF 2201 bp
Locus ID 270685
Cytogenetics 10 A1
MW 79.5 kDa
Summary May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.UniProtKB/Swiss-Prot Function
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"BC030437" in other vectors (4)
SKU Description Size Price
MC220946 Mthfd1l (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413),, (10ug) 10 ug
$739.00
MG210353 Mthfd1l (tGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) 10 ug
$871.00
MR210353L3 Lenti ORF clone of Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) 10 ug
$971.00
MR210353L4 Lenti ORF clone of Mthfd1l (mGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) 10 ug
$971.00

Plasmid Map for MR210353

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210353
Circular map for MR210353

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