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Mthfd1l (BC030437) Mouse Untagged Clone

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SKU
MC220946
Mthfd1l (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413),, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $739.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mthfd1l
Synonyms 2410004L15Rik; AI647056; Fthfsdc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC030437
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCCAGACAAGCTTCGAGAGGCAGACATTGTGGTCT
TAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGCGTGGATTCCATCAGGAACCACCATTCTCAATTGCTT
CCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTCCTGGGGTCCCCGTGGACAAACTCATTGCAGAA
GAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCAGAACATGGTGAGCAGCGGCAGGAGGTGGCTCA
GAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTGAAACTGCAGCCTCTCTCACCTGTGCCCAGTGA
TATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGGATGTCCTTGCCAAGGAGATAGGATTGCTTGCA
GATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGTCCAATTGTCCCTGCTGGAGAGGTTAAAGGATC
AAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACGCCCACCCCGCTCGGAGAAGGGAAGAGTACAGT
CACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGAAAGTCAACTCCTTCGCCTGTCTGAGGCAGCCT
TCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGCAGGAGGTGGATATGCTCAGGTCATCCCCATGG
AGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCCATCACTGCCGCTAATAACTTGCTGGCCGCAGC
CATCGACACGAGAATTTTACATGAGAGCACTCAAACAGACAAGGCTCTCTACAATCGACTGGTTCCCTTA
GTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTCTCGGCTGAAGAAACTGGGGATACATAAGACTG
ATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTCGCCCGCCTCAACATTGACCCCGCAACCATCAC
GTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGAGAAAAATAACCATTGGACAGGGAAGTACAGAG
AAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGTGGCCAGCGAGATCATGGCCGTGCTGGCCTTGA
CTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGAATGGTGGTGGCCAGTGACAAAGACGGGCAGCC
TGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGACAGTTTTGATGAAAGACGCCATCAAGCCAAAT
CTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCATGCTGGTCCCTTTGCCAACATCGCTCACGGCA
ACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTGGTCGGCGAAGAAGGGTTTGTGGTGACTGAAGC
TGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCAATATCAAATGCCGAGCTTCTGGCCTGGTGCCC
AACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAAGATGCATGGAGGCGGGCCGAGTGTAACTGCTG
GGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATTCAGCTGGTAGCTGATGGCTGCTGCAACCTACA
GAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGGTGGTGGTGGCACTGAATGTCTTCAAGACGGAC
ACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAAACGGGCCGGTGCCTTTGATGCGGTCCCCTGCT
ATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTGGCCCGGGCTGTGAGAGAAGCTGCGAATAAAAG
AAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCATCGTGGAAAAGATAAGAGTCATTGCCCAGACT
GTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGCGCAGTCCAAAATCGATCGCTATACGCAACAGG
GTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCACCTCTCTCTCTCTCATGAACCTGACAAAAAGGG
AGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCCGGGCCAGCATCGGGGCTGGGTTCATTTATCCT
TTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCACCCGACCCTGCTTTTATGATATAGACCTTGACA
CAGAAACGGAGCAAGTTAAAGGCTTGTTCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC030437
Insert Size 2202 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC030437, AAH30437
RefSeq Size 3061 bp
RefSeq ORF 2201 bp
Locus ID 270685
Cytogenetics 10 A1
Summary May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.UniProtKB/Swiss-Prot Function
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"BC030437" in other vectors (4)
SKU Description Size Price
MG210353 Mthfd1l (tGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) 10 ug
$871.00
MR210353 Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) 10 ug
$589.00 $671.00
MR210353L3 Lenti ORF clone of Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) 10 ug
$971.00
MR210353L4 Lenti ORF clone of Mthfd1l (mGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413) 10 ug
$971.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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