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Fchsd2 (NM_199012) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210309
Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $665.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fchsd2
Synonyms BC034086; mKIAA0769; R74866; Sh3md3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210309 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGACTTTCAGTCAGAAGAAGGCTGCTATTGAAAGAGAGTATGCACAGGGTATCCAGAAGTTGGCTA
GTCAATACCTGAAGAGAGATTGGCCTGGAATAAAAACTGATGATCGGAATGATTACAGGAGTATGTATCC
TGTTTGGAAATCTTTTCTCGAGGGAACAATGCAGGTAGCCCAGTCTCGGATCAATATATGTGAAAACTAT
AAAAACTTCATTTCTGAACCTGCAAGAGCAGTAAGAAGCTTAAAAGAACAGCAACTAAAACGGTGTGTGG
ATCAGTTGACAAAGATCCAAACAGAATTACAGGAAACTGTGAAAGATTTAGTGAAAGGCAAAAAGAAGTA
CTTTGAGACTGAACAGATGGCTCATGCAGTAAGAGAGAAAGCTGACATCGAAGCAAAGTCTAAACTTAGT
CTTTTTCAATCAAGAATCAGTTTACAGAAAGCAAGTGTGAAGTTAAAAGCCCGGCGATCTGAATGTAATA
CCAAAGCTACCCATGCAAGGAATGATTACCTTCTGACGCTAGCAGCAGCTAACGCACATCAGGACCGCTA
CTATCAAACAGACTTAGTTAACATCATGAAGGCTCTTGATGGAAACGTGTATGACCACCTCAAGGATTAT
TTAATAGCCTTCAGCCGGACCGAGCTAGAAACTTGCCAAGCTATACAGAATACATTCCAGTTTTTACTAG
AAAACTCCAGCAAGGTGGTACGGGACTATAACCTTCAGCTGTTTCTACAAGAGAATGCTGTATTTCACAA
GCCTCAGCCCTTCCAGTTCCAGCCTTGTGACAGTGATACTAGTTTAAAAGCCGCCCAACTGGTGGATATT
GAGCTCTCCCCTGTCAGTGCTCTGAGAATGACGATAGCTGAGAGTCGACAATTAGAATCAGAAACTGGTA
CCACAGAGGAACACAGTCTAAATAAGGAGGCTCGGAAATGGGCCACACGTGTGGCACGTGAGCACAAAAA
TATTGTTCATCAGCAGCGGGTTCTAAATGAACTAGAATGTCATGGAGTTGCTCTATCAGAACAGAGCCGA
GCAGAGCTGGAACAGAAAATAGATGAAGCAAGAGAAAGTATCCGTAAAGCAGAGATCATTAAATTGAAAG
CTGAAGCCCGGCTAGACCTACTGAAGCAGATTGGGGTTTCTGTAGATACGTGGTTAAAGAGTGCCATGAA
CCAAGTGATGGAGGAACTAGAAAATGAGCGGTGGGCCCGTCCTCCAGCCGTGACCAGCAATGGCACTCTA
CACTCGCTTAATGCAGATGCTGAAAGAGAAGAAGGAGAAGAGTTTGAAGACAACATGGATGTTTTTGATG
ACAGCAGCTCCAGCCCTTCTGGCACCTTGAGAAATTATCCACTCACCTGCAAAGTTGTTTATTCCTATAA
GGCTTCTCAACCAGATGAGTTGACCATTGAGGAACATGAGGTGTTAGAAGTGATTGAAGATGGAGATATG
GAAGACTGGGTAAAGGCTCGAAATAAAGTTGGCCAAGTGGGTTATGTGCCAGAAAAGTACCTACAATTTC
CCACCTCGAACAGCCTACTGAGCATGCTACAGTCTTTGGCTGCTTTGGACAGTCGGTCACACACATCCAG
CAATTCCACAGAAGCAGAACTCGTTTCAGGCAGCCTGAATGGAGATGCCAGTGTCTGTTTTGTGAAAGCA
CTTTATGATTATGAGGGCCAGACAGATGATGAGCTGTCCTTTCCCGAGGGAGCAATTATTCGCATCTTGA
ACAAAGAAAACCAAGATGATGATGGCTTCTGGGAAGGGGAGTTCAGTGGTCGAATCGGTGTTTTCCCATC
GGTGCTAGTGGAAGAGCTCTCAGCCTCCGAGAATGGCGACACTCCATGGACAAGAGAGATCCAGATCTCT
CCATCCCCCAAGCCTCACACATCCCTGCCTCCACTGCCTCTGTATGACCAGCCACCCAGCAGCCCGTATC
CCAGTCCAGATAAGAGGAGCTCCCAGTTCTTCCCCCGGTCTCCTTCAGCCAATGAAAACAGCCTTCATGC
TGAATCACCAGGATTCTCACAGGCCTCAAGACAGACTCCTGACACCTCATATGGCAAGCTGCGGCCTGTT
CGGGCGGCGCCGCCACCACCCACACAGAACCACCGGCGGACAACTGAGAAGATGGAGGACGTGGAGATCA
CACTGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210309 protein sequence
Red=Cloning site Green=Tags(s)

MRTFSQKKAAIEREYAQGIQKLASQYLKRDWPGIKTDDRNDYRSMYPVWKSFLEGTMQVAQSRINICENY
KNFISEPARAVRSLKEQQLKRCVDQLTKIQTELQETVKDLVKGKKKYFETEQMAHAVREKADIEAKSKLS
LFQSRISLQKASVKLKARRSECNTKATHARNDYLLTLAAANAHQDRYYQTDLVNIMKALDGNVYDHLKDY
LIAFSRTELETCQAIQNTFQFLLENSSKVVRDYNLQLFLQENAVFHKPQPFQFQPCDSDTSLKAAQLVDI
ELSPVSALRMTIAESRQLESETGTTEEHSLNKEARKWATRVAREHKNIVHQQRVLNELECHGVALSEQSR
AELEQKIDEARESIRKAEIIKLKAEARLDLLKQIGVSVDTWLKSAMNQVMEELENERWARPPAVTSNGTL
HSLNADAEREEGEEFEDNMDVFDDSSSSPSGTLRNYPLTCKVVYSYKASQPDELTIEEHEVLEVIEDGDM
EDWVKARNKVGQVGYVPEKYLQFPTSNSLLSMLQSLAALDSRSHTSSNSTEAELVSGSLNGDASVCFVKA
LYDYEGQTDDELSFPEGAIIRILNKENQDDDGFWEGEFSGRIGVFPSVLVEELSASENGDTPWTREIQIS
PSPKPHTSLPPLPLYDQPPSSPYPSPDKRSSQFFPRSPSANENSLHAESPGFSQASRQTPDTSYGKLRPV
RAAPPPPTQNHRRTTEKMEDVEITLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199012
ORF Size 2178 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199012.1, NP_950177.1
RefSeq Size 4432 bp
RefSeq ORF 2295 bp
Locus ID 207278
UniProt ID Q3USJ8
Cytogenetics 7 E2
MW 82.4 kDa
Summary Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor. Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling. Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits. Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (By similarity). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex (PubMed:23437151). Does not promote actin polymerisation in the absence of membranes (By similarity).UniProtKB/Swiss-Prot Function
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"NM_199012" in other vectors (4)
SKU Description Size Price
MC221418 Fchsd2 (untagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1, (10ug) 10 ug
$770.00
MG210309 Fchsd2 (tGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2) 10 ug
$866.00
MR210309L3 Lenti ORF clone of Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 10 ug
$966.00
MR210309L4 Lenti ORF clone of Fchsd2 (mGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 10 ug
$966.00

Plasmid Map for MR210309

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210309
Circular map for MR210309

file-alt Citations

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