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Fchsd2 (NM_199012) Mouse Untagged Clone

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SKU
MC221418
Fchsd2 (untagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $770.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Fchsd2
Synonyms BC034086; mKIAA0769; R74866; Sh3md3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221418 representing NM_199012
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCGCCGCCGAGGAAGGTGAAAGTTACACAAGAGCTGAGAAACATTCAAGGTGAGCAGATGACAA
AACTTCAAGCCAAACATCAGGCAGAATGTGACTTACTTGAAGACATGAGGACTTTCAGTCAGAAGAAGGC
TGCTATTGAAAGAGAGTATGCACAGGGTATCCAGAAGTTGGCTAGTCAATACCTGAAGAGAGATTGGCCT
GGAATAAAAACTGATGATCGGAATGATTACAGGAGTATGTATCCTGTTTGGAAATCTTTTCTCGAGGGAA
CAATGCAGGTAGCCCAGTCTCGGATCAATATATGTGAAAACTATAAAAACTTCATTTCTGAACCTGCAAG
AGCAGTAAGAAGCTTAAAAGAACAGCAACTAAAACGGTGTGTGGATCAGTTGACAAAGATCCAAACAGAA
TTACAGGAAACTGTGAAAGATTTAGTGAAAGGCAAAAAGAAGTACTTTGAGACTGAACAGATGGCTCATG
CAGTAAGAGAGAAAGCTGACATCGAAGCAAAGTCTAAACTTAGTCTTTTTCAATCAAGAATCAGTTTACA
GAAAGCAAGTGTGAAGTTAAAAGCCCGGCGATCTGAATGTAATACCAAAGCTACCCATGCAAGGAATGAT
TACCTTCTGACGCTAGCAGCAGCTAACGCACATCAGGACCGCTACTATCAAACAGACTTAGTTAACATCA
TGAAGGCTCTTGATGGAAACGTGTATGACCACCTCAAGGATTATTTAATAGCCTTCAGCCGGACCGAGCT
AGAAACTTGCCAAGCTATACAGAATACATTCCAGTTTTTACTAGAAAACTCCAGCAAGGTGGTACGGGAC
TATAACCTTCAGCTGTTTCTACAAGAGAATGCTGTATTTCACAAGCCTCAGCCCTTCCAGTTCCAGCCTT
GTGACAGTGATACTAGTTTAAAAGCCGCCCAACTGGTGGATATTGAGCTCTCCCCTGTCAGTGCTCTGAG
AATGACGATAGCTGAGAGTCGACAATTAGAATCAGAAACTGGTACCACAGAGGAACACAGTCTAAATAAG
GAGGCTCGGAAATGGGCCACACGTGTGGCACGTGAGCACAAAAATATTGTTCATCAGCAGCGGGTTCTAA
ATGAACTAGAATGTCATGGAGTTGCTCTATCAGAACAGAGCCGAGCAGAGCTGGAACAGAAAATAGATGA
AGCAAGAGAAAGTATCCGTAAAGCAGAGATCATTAAATTGAAAGCTGAAGCCCGGCTAGACCTACTGAAG
CAGATTGGGGTTTCTGTAGATACGTGGTTAAAGAGTGCCATGAACCAAGTGATGGAGGAACTAGAAAATG
AGCGGTGGGCCCGTCCTCCAGCCGTGACCAGCAATGGCACTCTACACTCGCTTAATGCAGATGCTGAAAG
AGAAGAAGGAGAAGAGTTTGAAGACAACATGGATGTTTTTGATGACAGCAGCTCCAGCCCTTCTGGCACC
TTGAGAAATTATCCACTCACCTGCAAAGTTGTTTATTCCTATAAGGCTTCTCAACCAGATGAGTTGACCA
TTGAGGAACATGAGGTGTTAGAAGTGATTGAAGATGGAGATATGGAAGACTGGGTAAAGGCTCGAAATAA
AGTTGGCCAAGTGGGTTATGTGCCAGAAAAGTACCTACAATTTCCCACCTCGAACAGCCTACTGAGCATG
CTACAGTCTTTGGCTGCTTTGGACAGTCGGTCACACACATCCAGCAATTCCACAGAAGCAGAACTCGTTT
CAGGCAGCCTGAATGGAGATGCCAGTGTCTGTTTTGTGAAAGCACTTTATGATTATGAGGGCCAGACAGA
TGATGAGCTGTCCTTTCCCGAGGGAGCAATTATTCGCATCTTGAACAAAGAAAACCAAGATGATGATGGC
TTCTGGGAAGGGGAGTTCAGTGGTCGAATCGGTGTTTTCCCATCGGTGCTAGTGGAAGAGCTCTCAGCCT
CCGAGAATGGCGACACTCCATGGACAAGAGAGATCCAGATCTCTCCATCCCCCAAGCCTCACACATCCCT
GCCTCCACTGCCTCTGTATGACCAGCCACCCAGCAGCCCGTATCCCAGTCCAGATAAGAGGAGCTCCCAG
TTCTTCCCCCGGTCTCCTTCAGCCAATGAAAACAGCCTTCATGCTGAATCACCAGGATTCTCACAGGCCT
CAAGACAGACTCCTGACACCTCATATGGCAAGCTGCGGCCTGTTCGGGCGGCGCCGCCACCACCCACACA
GAACCACCGGCGGACAACTGAGAAGATGGAGGACGTGGAGATCACACTGGTGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_199012
Insert Size 2295 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199012.2, NP_950177.2
RefSeq Size 4432 bp
RefSeq ORF 2295 bp
Locus ID 207278
UniProt ID Q3USJ8
Cytogenetics 7 E2
Summary Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor. Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling. Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits. Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (By similarity). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex (PubMed:23437151). Does not promote actin polymerisation in the absence of membranes (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_199012" in other vectors (4)
SKU Description Size Price
MG210309 Fchsd2 (tGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2) 10 ug
$866.00
MR210309 Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 10 ug
$589.00 $665.00
MR210309L3 Lenti ORF clone of Fchsd2 (Myc-DDK-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 10 ug
$966.00
MR210309L4 Lenti ORF clone of Fchsd2 (mGFP-tagged) - Mouse FCH and double SH3 domains 2 (Fchsd2), transcript variant 1 10 ug
$966.00

file-alt Citations

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