Nckipsd (NM_030729) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR210218
Nckipsd (Myc-DDK-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd)
$589.00
$654.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Nckipsd |
| Synonyms | AF3P21; DIP1; ORF1; SPIN90; Wasbp; WASLBP; WISH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_030729 |
| ORF Size | 2142 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_030729.4, NP_109654.2 |
| RefSeq Size | 2870 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 80987 |
| UniProt ID | Q9ESJ4 |
| Cytogenetics | 9 F2 |
| MW | 78.6 kDa |
| Summary | Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1) (By similarity). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_030729" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC221025 | Nckipsd (untagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd), (10ug) | 10 ug |
$720.00
|
|
| MG210218 | Nckipsd (tGFP-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd) | 10 ug |
$854.00
|
|
| MR210218L3 | Lenti ORF clone of Nckipsd (Myc-DDK-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd) | 10 ug |
$954.00
|
|
| MR210218L4 | Lenti ORF clone of Nckipsd (mGFP-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd) | 10 ug |
$954.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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