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Nckipsd (NM_030729) Mouse Untagged Clone

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SKU
MC221025
Nckipsd (untagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $720.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nckipsd
Synonyms AF3P21; DIP1; ORF1; SPIN90; Wasbp; WASLBP; WISH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221025 representing NM_030729
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCGCGCGCTGTATGCGTTCCGCTCCGCGGAGCCGAACGCCATGGCGTTCGCTGCAGGCGAGACCT
TCCTGGTGCTGGAGCGCAGCAGCACGCACTGGTGGTTAGCAGCACGGGCGCGCAGTGGCGAGACCGGGTA
CGTGCCGCCTGCCTACCTGCATCGCCTGCAGGGCATGGAACAAGATGTCCTCCAAGCTATTGACCGTGCC
ATTGAGGCTGTGCACAACACAGCCATGCGAGATGGCGGCAAGTACAGCCTGGAACAGCGTGGAGTCCTTC
AGAAGCTTATCCATCACCGGAAAGAAACCTTGTCTCGAAGAGGCACCTCAGCTTCCAGTGCTACGGTTAT
GACCCCATCCACCAGTGACCACCATTTGGACGCTGCTGTATCTAGACAGCCCAATGGGGTGTGTCGAACT
GGGTTTGAACGGCAGCATAGCCTGCCCAGTTCTGAGCATCTTGGGACAGATGGAGCCCTCTACCAGGTTC
CACCACAGCCTCGACGAGCAGCTCCTACCACCCCACCCCCACCGGTGAAGCGCCGAGACCGTGAGGCCCT
GGTGATCTCAGGGAGTGGTGGCCGCACAGCCATACCCTCTGGAGGTAGTTCTGTGTCTAGTGGCTCATCA
GCCAGCAGCACCTCCATGGACACACTCTATACTGGCTCCAGCCCCTCTGAGCTAGGTCCCAGCTGCTCAC
CCACACCCCCGCCGGTACCTCGCCGAGGGGCCCACACTACTGTGTCCCAGCCCCAGCCCTCTCCCTCCAA
GGCACCATCCCCTGAGCCCCCTACGGAGGAGGTGGCAGCTGAGACAAACTCAACCCCTGATGACTTGGAA
GCTCAGGATGCCCTGAGCCCAGAGACCACAGAGGAGAAGGCAGCCGCTGAGACAGTTGTGCCAAGGACCA
TTGGGGCAGAGCTGATGGAACTCGTGCGGAGAAACACCGGTCTAAGCCATGAGTTATGCCGTGTAGCCAT
CGGCGTCGTGGTGGGTCACATCCAGGCCACCGTGCCAGCCAGCTCACCTATCATGGAGCAGGTCCTCCTC
TCGCTGGTAGAAGGCAAGGACCTGAGCACAGCCCTACCCTCAGGGCAGGTCTGCCACGACCAGCAGAGAC
TGGAAGTGATTTTTGCAGACCTGGCTCGAAGAAAGGATGACGCCCAGCAGCGCAGCTGGGCGCTGTACGA
GGACGAGGACGTCATCCGCTGCTATCTGGAAGAACTGCTGCACATCCTGACGGATGCAGATCCCGAAGTT
TGCAAGAAAATGTGCAAAAGGAGTGATTTTGAGTCTGTCCTAGCCTTGGTGGCCTATTACCAAATGGAAC
ACCGAGCGTCTCTACGGCTGCTGCTCCTCAAGTGTTTCGGTGCCATGTGCAGCCTAGATGCAGCGATCAT
CTCCACTCTGGTGTCTTCCGTGCTGCCTGTGGAATTGGCACGGGACATGCAGACCGACACGCAGGACCAC
CAGAAGCTCTGTTACTCTGCCCTCGTCCTGGCCATGGTCTTCTCCATGGGAGAGGCGGTACCCTACGCAC
ACTATGAACACCTGGGTACACCCTTTGCCCAGTTCCTACTAAGCATCGTTGAGGATGGCCTTCCAATGGA
CACCACGGAGCAGCTGCCAGACCTCTGCATGAACCTGCTTCTGGCTCTCAACCTGCACCTGACAGCTCCT
GAGCAGAATGTCATCATGGCTGCCTTGAGCAGACACACCAATGTGAAGATCTTCTCTGAGAAGCTGCTTC
TGCTTCTGAACAGAGGGGACGACCCTGTGCGTATCTTCAGACATGAGCCCCAGCCACCACACTCTGTCCT
CAAGTTCCTGCAGGATGTGTTCAGCAGCTCTGCCACAGCCGCCATCTTCTACCACACAGACATGATGGCG
CTTATTGACATCACTGTGCGCCAGATCGCAGACCTGTCACCTGGAGACAAGCTACGCATGGAGTACCTCT
CCCTGATGCATGCTGTGGTCCGCTCCACGCCCTACCTGCAGCACCGACATCGGCTGTCAGACCTGCAGGC
TACACTGCGGCGCATCCTGACTGAGGAAGAGGCCTCACCCCAGTGCCAGATGGACCGTATGATTGTCCAA
GAGATGTACAAGGAATTCCCGGACCTGGGAGAGGTTCCCAGCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_030729
Insert Size 2145 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030729.4, NP_109654.2
RefSeq Size 2870 bp
RefSeq ORF 2145 bp
Locus ID 80987
UniProt ID Q9ESJ4
Cytogenetics 9 F2
Summary Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1) (By similarity). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.UniProtKB/Swiss-Prot Function
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"NM_030729" in other vectors (4)
SKU Description Size Price
MG210218 Nckipsd (tGFP-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd) 10 ug
$854.00
MR210218 Nckipsd (Myc-DDK-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd) 10 ug
$589.00 $654.00
MR210218L3 Lenti ORF clone of Nckipsd (Myc-DDK-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd) 10 ug
$954.00
MR210218L4 Lenti ORF clone of Nckipsd (mGFP-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd) 10 ug
$954.00

file-alt Citations

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