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Ift81 (NM_009879) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209962
Ift81 (Myc-DDK-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $620.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ift81
Synonyms AW060663; Cdv-1; Cdv-1r; Cdv1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209962 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGACCAAATCAAATTCATCGTGGACAGCCTCAATAAGGAGCCTTTTAAGAAGAATTATAACTTAA
TCACATTTGATTCTTTGGGACCAATGCAACTATTGCAAGTTCTCAATGATGTCCTGGCTGAGATTGACCC
CAAGCAAGATGTTGATATCAGAGAGGAGATGCCAGAGCAGACAGCCAAACGAATGTTGAATCTTCTTGGT
ATCCTTAAGTACAAACCTCCGGGAAATGCCACAGACATGAGCACCTTCCGTCAGGGCTTGGTGATTGGAA
GTAAACCTGTGATTTACCCAGTGCTCCACTGGCTTCTTCAGCGGAGTAATGAACTAAAGAAAAGGGCATA
TTTAGCTCGTTTCTTAATAAAACTGGAGGTGCCAAGTGAGTTTCTTCAAGATGAGACCGTGGCTGACACC
AATAAACAGTATGAAGAGCTGATGGAAGCCTTTAAAACTCTGCATAAAGAGTGTGAGCAGCTCAAAACAT
CTGGATTCTCTACAGCAGAAATCAGAAGGGATATCAGCGCAATGGAGGAGGAAAAAGACCAGCTCATGAA
GAGAGTTGAACGGCTGAAGAAGAGGGTGGAGACCGTTCAGAACCATCAGCGAATGCTTAAAATAGCAAGA
CAGCTTCGAGTTGAGAAAGAGAGAGAAGAATTTCTTGCACAACAGAAACAGGAACAAAAGAATCAGCTGT
TTCATGCGGTGCAGAGATTGCAGAGAGTGCAAAATCAGCTGAAGAGCATGCGGCACGCAGCAGCGGATGC
AAAGCCTGAGAGTTTAATGAAGAGGCTAGAGGAGGAGATAAAATTTAACTCATACATGGTCACTGAAAAA
TTTCCAAAAGAATTAGAAAGCAAGAAAAAGGAATTACATTTTTTACAGAAGGTGGTTTCTGAGCCAGCTA
TGGGCCACTCTGACCTTCTCGAACTTGAGACTAAAGTCAATGAAGTAAACACGGAGATCAATCAGTTGAT
TGAAAAGAAAATGATGAGAAACGAGCCAATTGAAGGCAAGCTCTCACTGTACAGGCAACAGGCATCCATC
ATTTCTCGTAAGAAAGAAGCCAAAGCTGAGGAGCTGCAGGAGACCAAGGAGAAGCTGGCCAGCCTTGAGA
GAGAGGTGTTGGTGAAAACAAACCAGACCCGGGAGTTCGACGGCACTGAGGTTCTGAAGGGAGATGAGTT
CAAGCGATACGTCAGTAAACTTCGAAGCAAAAGCACAGTTTTCAAAAAGAAGCATCAGATAATAGCTGAA
TTTAAGGCTGAGTTTGGCCTCCTGCAGAGGACAGAAGAACTTCTGAAGCAGCGTCAGGAAACCATTCAGC
ATCAACTGCGAACTATTGAAGAAAAGAAGGGTATATCTGGATACAGTTACACCCAAGAGGAGCTTGAGAG
AGTGTCGGCCCTGAAGAGTGAGGTTGACGAGATGAAAGGCCGGACCCTGGATGACATGTCAGAGATGGTG
AAAAAACTGAATTCCTTGGTATCAGAAAAGAAGTCAGCTTTAGCCCCAGTTATAAAAGAACTACGGCAGT
TGCGTCAGAAATGTCAAGAACTAACCCAGGAGTGTGATGAGAAGAAAGCCCAGTATGATAGCTGTGCAGC
AGGCCTTGAAAGCAACCGGTCCAAATTAGAGCAGGAAGTGCGAGGACTTCGTGAGGAATGTCTTCAAGAA
GAAAGTAAATACCACTATACAAACTGCATGATTAAGAACCTAGAAGTTGAGCTTCGTCGAGCGACTGATG
AGATGAAGGCGTACGTCTCTTCTGATCAGCAAGAGAAGAGGAAAGCAATTAGGGAACAGTATACCAAAAA
TATTACTGAGCAAGAAAACCTTGGTAAGAAACTCCGGGAAAAACAAAAAGCTGTACGAGAAAGTCATGGT
CCAAACATGAAGCAAGCAAAAATGTGGCGTGATCTTGAACAACTGATGGAATGCAAAAAACAGTGCTTTC
TAAAGCAGCAAAGCCCAGCTTCCATCGGCCAGGTAATTCAAGAGGGCGGAGAGGACCGGCTGGTTCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209962 protein sequence
Red=Cloning site Green=Tags(s)

MSDQIKFIVDSLNKEPFKKNYNLITFDSLGPMQLLQVLNDVLAEIDPKQDVDIREEMPEQTAKRMLNLLG
ILKYKPPGNATDMSTFRQGLVIGSKPVIYPVLHWLLQRSNELKKRAYLARFLIKLEVPSEFLQDETVADT
NKQYEELMEAFKTLHKECEQLKTSGFSTAEIRRDISAMEEEKDQLMKRVERLKKRVETVQNHQRMLKIAR
QLRVEKEREEFLAQQKQEQKNQLFHAVQRLQRVQNQLKSMRHAAADAKPESLMKRLEEEIKFNSYMVTEK
FPKELESKKKELHFLQKVVSEPAMGHSDLLELETKVNEVNTEINQLIEKKMMRNEPIEGKLSLYRQQASI
ISRKKEAKAEELQETKEKLASLEREVLVKTNQTREFDGTEVLKGDEFKRYVSKLRSKSTVFKKKHQIIAE
FKAEFGLLQRTEELLKQRQETIQHQLRTIEEKKGISGYSYTQEELERVSALKSEVDEMKGRTLDDMSEMV
KKLNSLVSEKKSALAPVIKELRQLRQKCQELTQECDEKKAQYDSCAAGLESNRSKLEQEVRGLREECLQE
ESKYHYTNCMIKNLEVELRRATDEMKAYVSSDQQEKRKAIREQYTKNITEQENLGKKLREKQKAVRESHG
PNMKQAKMWRDLEQLMECKKQCFLKQQSPASIGQVIQEGGEDRLVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009879
ORF Size 2028 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009879.4
RefSeq Size 2957 bp
RefSeq ORF 2031 bp
Locus ID 12589
UniProt ID O35594
Cytogenetics 5 62.44 cM
MW 79.3 kDa
Summary Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis. Required for proper regulation of SHH signaling.UniProtKB/Swiss-Prot Function
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"NM_009879" in other vectors (4)
SKU Description Size Price
MC205085 Ift81 (untagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81), (10ug) 10 ug
$620.00
MG209962 Ift81 (tGFP-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81) 10 ug
$820.00
MR209962L3 Lenti ORF clone of Ift81 (Myc-DDK-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81) 10 ug
$920.00
MR209962L4 Lenti ORF clone of Ift81 (mGFP-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81) 10 ug
$920.00

Plasmid Map for MR209962

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209962
Circular map for MR209962

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