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Ift81 (NM_009879) Mouse Untagged Clone

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SKU
MC205085
Ift81 (untagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $620.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ift81
Synonyms AW060663; Cdv-1; Cdv-1r; Cdv1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC055721
CCGAGGGTGGGGAGGTGGTTTCGTGAGGAGGATTTGAGTTTTAAGCTATAACATTAATCATGAGTGACCA AATCAAATTCATCGTGGACAGCCTCAATAAGGAGCCTTTTAAGAAGAATTATAACTTAATCACATTTGAT TCTTTGGGACCAATGCAACTATTGCAAGTTCTCAATGATGTCCTGGCTGAGATTGACCCCAAGCAAGATG TTGATATCAGAGAGGAGATGCCAGAGCAGACAGCCAAACGAATGTTGAATCTTCTTGGTATCCTTAAGTA CAAACCTCCGGGAAATGCCACAGACATGAGCACCTTCCGTCAGGGCTTGGTGATTGGAAGTAAACCTGTG ATTTACCCAGTGCTCCACTGGCTTCTTCAGCGGAGTAATGAACTAAAGAAAAGGGCATATTTAGCTCGTT TCTTAATAAAACTGGAGGTGCCAAGTGAGTTTCTTCAAGATGAGACCGTGGCTGACACCAATAAACAGTA TGAAGAGCTGATGGAAGCCTTTAAAACTCTGCATAAAGAGTGTGAGCAGCTCAAAACATCTGGATTCTCT ACAGCAGAAATCAGAAGGGATATCAGCGCAATGGAGGAGGAAAAAGACCAGCTCATGAAGAGAGTTGAAC GGCTGAAGAAGAGGGTGGAGACCGTTCAGAACCATCAGCGAATGCTTAAAATAGCAAGACAGCTTCGAGT TGAGAAAGAGAGAGAAGAATTTCTTGCACAACAGAAACAGGAACAAAAGAATCAGCTGTTTCATGCGGTG CAGAGATTGCAGAGAGTGCAAAATCAGCTGAAGAGCATGCGGCACGCAGCAGCGGATGCAAAGCCTGAGA GTTTAATGAAGAGGCTAGAGGAGGAGATAAAATTTAACTCATACATGGTCACTGAAAAATTTCCAAAAGA ATTAGAAAGCAAGAAAAAGGAATTACATTTTTTACAGAAGGTGGTTTCTGAGCCAGCTATGGGCCACTCT GACCTTCTCGAACTTGAGACTAAAGTCAATGAAGTAAACACGGAGATCAATCAGTTGATTGAAAAGAAAA TGATGAGAAACGAGCCAATTGAAGGCAAGCTCTCACTGTACAGGCAACAGGCATCCATCATTTCTCGTAA GAAAGAAGCCAAAGCTGAGGAGCTGCAGGAGACCAAGGAGAAGCTGGCCAGCCTTGAGAGAGAGGTGTTG GTGAAAACAAACCAGACCCGGGAGTTCGACGGCACTGAGGTTCTGAAGGGAGATGAGTTCAAGCGATACG TCAGTAAACTTCGAAGCAAAAGCACAGTTTTCAAAAAGAAGCATCAGATAATAGCTGAATTTAAGGCTGA GTTTGGCCTCCTGCAGAGGACAGAAGAACTTCTGAAGCAGCGTCAGGAAACCATTCAGCATCAACTGCGA ACTATTGAAGAAAAGAAGGGTATATCTGGATACAGTTACACCCAAGAGGAGCTTGAGAGAGTGTCGGCCC TGAAGAGTGAGGTTGACGAGATGAAAGGCCGGACCCTGGATGACATGTCAGAGATGGTGAAAAAACTGAA TTCCTTGGTATCAGAAAAGAAGTCAGCTTTAGCCCCAGTTATAAAAGAACTACGGCAGTTGCGTCAGAAA TGTCAAGAACTAACCCAGGAGTGTGATGAGAAGAAAGCCCAGTATGATAGCTGTGCAGCAGGCCTTGAAA GCAACCGGTCCAAATTAGAGCAGGAAGTGCGAGGACTTCGTGAGGAATGTCTTCAAGAAGAAAGTAAATA CCACTATACAAACTGCATGATTAAGAACCTAGAAGTTGAGCTTCGTCGAGCGACTGATGAGATGAAGGCG TACGTCTCTTCTGATCAGCAAGAGAAGAGGAAAGCAATTAGGGAACAGTATACCAAAAATATTACTGAGC AAGAAAACCTTGGTAAGAAACTCCGGGAAAAACAAAAAGCTGTACGAGAAAGTCATGGTCCAAACATGAA GCAAGCAAAAATGTGGCGTGATCTTGAACAACTGATGGAATGCAAAAAACAGTGCTTTCTAAAGCAGCAA AGCCCAGCTTCCATCGGCCAGGTAATTCAAGAGGGCGGAGAGGACCGGCTGGTTCTCTGAGCCCTGTGTC GGCCGGGCTTAAGTCGCATCTGCGTAACGTGATTTCTTCTCGGAAGTGACTTATTCATCCTTTGCTTTCA GACTAGCCGTCCCTTCTCAGTTGTCTTGGAAATGAAGTGAAGGCAGATGAGGTTTTAAAGGTCTTTTTAA CTGCAGGAGAGGCTTTCGTTGTTTGTTTAGCTTAGCCGTCTAGTGGTGTCTCCTGAGTTTTATGGGAAAG GGCTAAGTTTGCTCTCTGTAATGTAAGCATGTGTCCATTAAGAACCACGTAGCTTCTCAAGCTGTGAGGA CTGACGGGTAATGCTCTTCTCAGTCTGTCTCTGACTTGGAGGACTCTTTTAGGAACTAGGAGTTCGTTTG AAGAAGCTTTTGTCTGAAGTCAGCAGTCGCTACAGTTTCACTTCAGTTTCTTTCTCTTCTATAAAATAAA ACCAGTTAATGTTGTGAAAAGCAATTGTGATGTGATGTGATTGCAAAGTAAATAAAGCCTGCGGTGGCCT GCCGTAGCTGTTGATCTTAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_009879
Insert Size 2031 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC055721, AAH55721
RefSeq Size 2627 bp
RefSeq ORF 2031 bp
Locus ID 12589
UniProt ID O35594
Cytogenetics 5 62.44 cM
Summary Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis. Required for proper regulation of SHH signaling.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_009879" in other vectors (4)
SKU Description Size Price
MG209962 Ift81 (tGFP-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81) 10 ug
$820.00
MR209962 Ift81 (Myc-DDK-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81) 10 ug
$589.00 $620.00
MR209962L3 Lenti ORF clone of Ift81 (Myc-DDK-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81) 10 ug
$920.00
MR209962L4 Lenti ORF clone of Ift81 (mGFP-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81) 10 ug
$920.00

file-alt Citations

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