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Pus7 (NM_178403) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209845
Pus7 (Myc-DDK-tagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $615.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pus7
Synonyms C330017I15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209845 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCAC
ACGACGAAACAAAGAAACAGAAGGTGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGA
CGGTCTACACCGCAGTGAGAACGAGCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAAT
TCCAGCGCTCAGGTGCAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGACGGCCTTTCAGAAGCAGGGGAAG
AGGAAGAAGCAGAGAGTTTTGCAGACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAA
GTTCGTGAGTTCCCATCATGGCTTCTCAGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAA
ATCGGGAAAGATGGACGGATCAGCCATTTGGATGACTTATCTGTTCCGGTGGACGAAGAGGACCCTCCAG
AAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAA
AGAAACCAGTGTTGCCATCGAGGTCATTGAGGACACCAAAGAGAAGCGAACAGTCATCCATCAAGCCATT
AAATCTCTCTTCCCAGGACTAGAGACAAAGACAGAGGATAGAGAGGGGCGGAAATACATCGTAGCCTACC
ATGCAGCTGGCAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCCCGGGGAAGTTACTG
CCATTTTGTTCTCTACAAGGAAAACAAAGACACCATGGATGCCATCAATGTTCTATCCAAGTACCTGCGG
GTAAAGCCAAACATATTCTCCTATATGGGAACCAAAGACAAAAGGGCAATCACAGTCCAGGAGATTGCTG
TTCTCAAAATAAGTGCACAAAGACTTGCCCACCTCAATAAGTGCCTGATGAACTTGAAGCTCGGGAATTT
CAGCTATCAGAAAACCCCTCTGAAGTTAGGAGCACTCCAAGGAAACCACTTCACTGTTGTTCTCAGGAAT
ATAACAGGAACTGATGAGCAGGTGCAGCAAGCCATGCAGTCTCTGAGGGAGACCGGCTTCATTAACTACT
ATGGCATGCAAAGGTTCGGGACCACAGCTGTTCCAACGTACCAGGTTGGAAGAGCAATTCTACAGAATTC
CTGGACAGAAGTCATGGATTTAATACTGAAACCCCGTTCTGGAGCTGAGAAGGGTTACTTGGTGAAATGC
AGAGAAGAATGGGCCAAGACCAAAGACCCAGCCTCTGCTCTCAAAAAGCTGCCCGTCAAAAGGTGTGTGG
AAGGGCAGCTGCTCCGAGGACTTTCCAGATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAATTCC
AAGAAACAATCGCTTAATGTACATCCACAGTTATCAGAGCTATGTGTGGAACACCATGGTGAGCAGAAGA
ATTGAGGAGTACGGGCTGAGGCCTGTGCCCGGGGACCTTGTTCTCAAAGGAGCCACGCCCACCTACATTG
AGGAAGATGACGTTGACAACTACTCCATCCACGATGTGGTGATGCCTTTGCCTGGGTTTGATGTTATCTA
CCCCAAGCATAAAATCAGTGAGGCCTACAGGGAAATGCTTGCTGCAGACAACCTTGATATTGACAACATG
AGACACACGATTCGAGATTACTCCTTATCGGGGGCCTACAGAAGGATCATCATCAGGCCTCAGAGCGTCA
GCTGGGAAGTTGTTGCATATGATGATCCTAAAATTCCACTTTTCAACACGGATGTGGACAACCTCGAGGG
GAAACCACCACCAGTGTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAGATGGACTTTTCTCTTCCTCCT
TCTACTTACGCCACCATGGCCATTCGAGAAGTGCTCAAAATGGACACCAGCATCAAGAACCAGACCCAGC
TAAATACGAGCTGGCTGCGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209845 protein sequence
Red=Cloning site Green=Tags(s)

MEMTSTSLKRGCLVVEDNDSVTPHDETKKQKVSEGCLTSSQDGVENDGLHRSENEPGPPEAESTVKDDEN
SSAQVQEEEEEEEEEDGLSEAGEEEEAESFADMMKHGLTELDVGICKFVSSHHGFSGILKERYSDFVVHE
IGKDGRISHLDDLSVPVDEEDPPEDALTVLTAEDRQQLEELQLFKNKETSVAIEVIEDTKEKRTVIHQAI
KSLFPGLETKTEDREGRKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYLR
VKPNIFSYMGTKDKRAITVQEIAVLKISAQRLAHLNKCLMNLKLGNFSYQKTPLKLGALQGNHFTVVLRN
ITGTDEQVQQAMQSLRETGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVKC
REEWAKTKDPASALKKLPVKRCVEGQLLRGLSRYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNTMVSRR
IEEYGLRPVPGDLVLKGATPTYIEEDDVDNYSIHDVVMPLPGFDVIYPKHKISEAYREMLAADNLDIDNM
RHTIRDYSLSGAYRRIIIRPQSVSWEVVAYDDPKIPLFNTDVDNLEGKPPPVFASEGKYRALKMDFSLPP
STYATMAIREVLKMDTSIKNQTQLNTSWLR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178403
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178403.3, NM_178403.4, NM_178403.5, NP_848490.2
RefSeq Size 3907 bp
RefSeq ORF 1983 bp
Locus ID 78697
UniProt ID Q91VU7
Cytogenetics 5 A3
MW 74.8 kDa
Summary Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types.UniProtKB/Swiss-Prot Function
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"NM_178403" in other vectors (3)
SKU Description Size Price
MC203665 Pus7 (untagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7), (10ug) 10 ug
$615.00
MR209845L3 Lenti ORF clone of Pus7 (Myc-DDK-tagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7) 10 ug
$915.00
MR209845L4 Lenti ORF clone of Pus7 (mGFP-tagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7) 10 ug
$915.00

Plasmid Map for MR209845

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209845
Circular map for MR209845

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