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Pus7 (NM_178403) Mouse Untagged Clone

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SKU
MC203665
Pus7 (untagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7), (10ug)
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $615.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pus7
Synonyms C330017I15Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC008544
GGCTGGTGGGATAACCCGTCCCTGTGCGTCTCCACGCGGTGCAGCCTGTGCTGGTCCGCGCTGGCCGCCG CCTCCTGCGAGTCACCGCCGCCGCCCTCCCGCGTCCCGAGCCGAGCGTCCTCTTCGCCGCTCGGCCCGCG CCCTCAGGCTCCCGGTTCTCTTAAATGTTTTCTTGGAGCCTTAAAGATGGAAATGACAAGCACGTCCCTG AAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCACACGACGAAACAAAGAAACAGAAGG TGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGACGGTCTACACCGCAGTGAGAACGA GCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAATTCCAGCGCTCAGGTGCAAGAGGAA GAGGAGGAGGAGGAGGAGGAAGACGGCCTTTCAGAAGCAGGGGAAGAGGAAGAAGCAGAGAGTTTTGCAG ACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAAGTTCGTGAGTTCCCATCATGGCTT CTCAGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAAATCGGGAAAGATGGACGGATCAGC CATTTGGATGACTTATCTGTTCCGGTGGACGAAGAGGACCCTCCAGAAGACGCTCTCACTGTCTTGACAG CTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAAAGAAACCAGTGTTGCCATCGAGGT CATTGAGGACACCAAAGAGAAGCGAACAGTCATCCATCAAGCCATTAAATCTCTCTTCCCAGGACTAGAG ACAAAGACAGAGGATAGAGAGGGGCGGAAATACATCGTAGCCTACCATGCAGCTGGCAAAAAGGCTTTGG CAAATCCAAGAAAACATTCTTGGCCAAAATCCCGGGGAAGTTACTGCCATTTTGTTCTCTACAAGGAAAA CAAAGACACCATGGATGCCATCAATGTTCTATCCAAGTACCTGCGGGTAAAGCCAAACATATTCTCCTAT ATGGGAACCAAAGACAAAAGGGCAATCACAGTCCAGGAGATTGCTGTTCTCAAAATAAGTGCACAAAGAC TTGCCCACCTCAATAAGTGCCTGATGAACTTGAAGCTCGGGAATTTCAGCTATCAGAAAACCCCTCTGAA GTTAGGAGCACTCCAAGGAAACCACTTCACTGTTGTTCTCAGGAATATAACAGGAACTGATGAGCAGGTG CAGCAAGCCATGCAGTCTCTGAGGGAGACCGGCTTCATTAACTACTATGGCATGCAAAGGTTCGGGACCA CAGCTGTTCCAACGTACCAGGTTGGAAGAGCAATTCTACAGAATTCCTGGACAGAAGTCATGGATTTAAT ACTGAAACCCCGTTCTGGAGCTGAGAAGGGTTACTTGGTGAAATGCAGAGAAGAATGGGCCAAGACCAAA GACCCAGCCTCTGCTCTCAAAAAGCTGCCCGTCAAAAGGTGTGTGGAAGGGCAGCTGCTCCGAGGACTTT CCAGATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAATTCCAAGAAACAATCGCTTAATGTACAT CCACAGTTATCAGAGCTATGTGTGGAACACCATGGTGAGCAGAAGAATTGAGGAGTACGGGCTGAGGCCT GTGCCCGGGGACCTTGTTCTCAAAGGAGCCACGCCCACCTACATTGAGGAAGATGACGTTGACAACTACT CCATCCACGATGTGGTGATGCCTTTGCCTGGGTTTGATGTTATCTACCCCAAGCATAAAATCAGTGAGGC CTACAGGGAAATGCTTGCTGCAGACAACCTTGATATTGACAACATGAGACACACGATTCGAGATTACTCC TTATCGGGGGCCTACAGAAGGATCATCATCAGGCCTCAGAGCGTCAGCTGGGAAGTTGTTGCATATGATG ATCCTAAAATTCCACTTTTCAACACGGATGTGGACAACCTCGAGGGGAAACCACCACCAGTGTTTGCTTC TGAAGGCAAATACAGGGCTCTGAAGATGGACTTTTCTCTTCCTCCTTCTACTTACGCCACCATGGCCATT CGAGAAGTGCTCAAAATGGACACCAGCATCAAGAACCAGACCCAGCTAAATACGAGCTGGCTGCGATGAA CAGTGCATCGCTCAGAGAGGGGATGCAGACCTGCTTCTGACCTCCTATTCTGGTCTTTTTTTAAGTGCTG ACTCATGTTTGGAGTTTAAAACTTTTGTAGTAAAGGATGATTTGTATTTTGTAAAGTTTTTAATCAGCTT AAATACTTTGCAATATTTGTACACATATACAAGTCCTGAGGATCCTAATTTCAGCTCAGAGATAGAATTG GGTCAGCATATCCTGTAGGTGCTTAGATTCTAGTACAGCATCAGCTTCCTGCCTTTAACACAGGGAAGGG GGACTTGGTTTAAAGTTGCGGTTTTAGGTTTTGCTGCACACCTGAAGTACAAACCAGAATCTTAGATGTT TGTGATGAGATTACACATGGTCCGTGTCAGAGCAAATGCAGCAGACTGGAAACTAGAGACTGCACACTTA ACACGCAGTTCCCGTGTTGGTTTCAATAATAACAAAACATCCACGGGTTTATAAACACGGCGTGGCTTTT GTTGAGTGACTGTGTTAGCTTCAGGTGAGCATTTATACACATCTTAGCTTGCAGTGGCTTTTCTGGTTAG TCTATCAGCATCTTTCAGGGCAGTCTTTTCCTTGATAGGAGCCTGTAGCTTTTCTTGGGTACAGTCTGAT TTTACTCTGGGTGCCTCTTACTTTCAGAACCTTTTCACTGGACTCAAAAGTAAATAGTGTGAGTTCCAGG ACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAACGAAAAAAACAAACAAACAAAAGGTAAATAGTGAC CATGAAGGAAATCTGGGAAACTTAGAATGGCTCCTGCAGCCAGGCGTGGTGGCCACGCCTTTAATTCCAG CCCCCGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCAAGGCCATCCTGGTCTACAGAGTGAGTTTCAGGA CAGAGAAACCCTGTCTCAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_178403
Insert Size 1983 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC008544, AAH08544
RefSeq Size 3137 bp
RefSeq ORF 1983 bp
Locus ID 78697
UniProt ID Q91VU7
Cytogenetics 5 A3
Summary Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which lacks an internal segment and is shorter, compared to isoform 1.
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Documents
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"NM_178403" in other vectors (3)
SKU Description Size Price
MR209845 Pus7 (Myc-DDK-tagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7) 10 ug
$289.00 $615.00
MR209845L3 Lenti ORF clone of Pus7 (Myc-DDK-tagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7) 10 ug
$915.00
MR209845L4 Lenti ORF clone of Pus7 (mGFP-tagged) - Mouse pseudouridylate synthase 7 homolog (S. cerevisiae) (Pus7) 10 ug
$915.00

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