Arntl (NM_007489) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR209553
Arntl (Myc-DDK-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl)
$289.00
$584.00
2 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Arntl |
| Synonyms | Arnt3; bHLHe5; Bmal1; BMAL1b; bmal1b'; MOP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007489 |
| ORF Size | 1878 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007489.4 |
| RefSeq Size | 2921 bp |
| RefSeq ORF | 1881 bp |
| Locus ID | 11865 |
| UniProt ID | Q9WTL8 |
| Cytogenetics | 7 59.17 cM |
| MW | 68.7 kDa |
| Summary | The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with Clock. This heterodimer binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007489" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC205396 | Arntl (untagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl), (10ug) | 10 ug |
$584.00
|
|
| MG209553 | Arntl (tGFP-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl) | 10 ug |
$489.00
$784.00
|
|
| MR209553L3 | Lenti ORF clone of Arntl (Myc-DDK-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl) | 10 ug |
$884.00
|
|
| MR209553L4 | Lenti ORF clone of Arntl (mGFP-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator-like (Arntl) | 10 ug |
$884.00
|
|
Citations
Monocyte/Macrophage-Specific Loss of ARNTL Suppresses Chronic Kidney Disease-Associated Cardiac Impairment
Yoshida, Nishikawa, Fukuoka et al
Int J Mol Sci (2024) 25 (23) DOI: 10.3390/ijms252313009
Yoshida, Nishikawa, Fukuoka et al
Int J Mol Sci (2024) 25 (23) DOI: 10.3390/ijms252313009
Cited by 3
AI Summary: OriGene’s mouse ARNTL-expressing plasmid (MR209553) was used to generate RAW264.7 cells stably expressing ARNTL, enabling the study of ARNTL’s role in monocyte/macrophage-mediated cardiac impairment in chronic kidney disease.
Snippet: into RAW264.7 cells using Lipofectamine LTX and PLUS reagents (Thermo Fisher Scientific). 4.4. Construction of RAW264.7 Cells Stably Expressing ARNTL Mouse ARNTL-expressing plasmid (MR209553, OriGene Technologies, Inc., Rockville, MD, USA) was transfected into RAW264.7 cells using Lipofectamine LTX and PLUS reagents (Thermo Fisher Scientific). Cells stably expressing ARNTL were selec
S6K1 controls adiponectin expression by inducing a transcriptional switch: BMAL1-to-EZH2
Yi, Jeon, Lee et al
Exp Mol Med (2022) 54 (3), 324-333 DOI: 10.1038/s12276-022-00747-7
Yi, Jeon, Lee et al
Exp Mol Med (2022) 54 (3), 324-333 DOI: 10.1038/s12276-022-00747-7
Cited by 17
AI Summary: OriGene contributed the pCMV6-FLAG-BMAL1 plasmid (Cat. No. MR209553), which was used in this study to investigate the role of BMAL1 in the S6K1-mediated regulation of adiponectin expression.
Snippet: Table 1. The DNA constructs used in this study were pCDNA-EGFP-H2B, pCDNA-EGFP-H2BS36A, pCDNA-EGFP-H2BS36D, and pRK5-myc-S6K1-CA, which were previously described9. pCMV6-FLAG-BMAL1 was purchased from Origene (Rockville, MD, USA, Cat. No. MR209553). The mutant constructs for BMAL1 were generated using site-directed mutagenesis (pCMV6-FLAG-BMAL1-S42A). Inhibition of S6K1 First, 3T3-L1 adipocytes we
Loss of the transcriptional repressor Rev-erbα upregulates metabolism and proliferation in cultured mouse embryonic fibroblasts
Gillis, Yao, Rizal et al
Sci Rep (2021) 11 (1), 12356 DOI: 10.1038/s41598-021-91516-5
Gillis, Yao, Rizal et al
Sci Rep (2021) 11 (1), 12356 DOI: 10.1038/s41598-021-91516-5
Cited by 6
AI Summary: OriGene’s pCMV6 vector plasmid (catalog # MR209553) was used to overexpress Arntl in wild-type mouse embryonic fibroblasts, enabling the study of its role in Rev-erbα-mediated regulation of metabolism and proliferation.
Snippet: Thermo Fisher Ambion siRNA (catalog # AM16708) was used for ribose-5-phosphate isomerase (RPIA) silencing. Arntl was overexpressed in WT MEFs using an Origene pCMV6 vector plasmid (catalog # MR209553) using Thermo Fisher Lipofectamine 3000 reagent
The Role of BMAL1 in Linking the Molecular Clock and Circadian Rhythm of Cerebral Myogenic Tone
Shaw
Thesis (2021)
Shaw
Thesis (2021)
Snippet: Commercially available CMV-driven mouse Bmal1 expression vector Origene, Rockville, USA, #MR209553
The Circadian Clock Regulates Adipogenesis by a Per3 Crosstalk Pathway to Klf15
Aggarwal, Costa, Rivero-Gutiérrez et al
Cell Rep (2017) 21 (9), 2367-2375 DOI: 10.1016/j.celrep.2017.11.004
Aggarwal, Costa, Rivero-Gutiérrez et al
Cell Rep (2017) 21 (9), 2367-2375 DOI: 10.1016/j.celrep.2017.11.004
AI Summary: OriGene’s pCMV6-Bmal1-Myc construct was used in this study to investigate the role of BMAL1 in the regulation of adipogenesis and its interaction with PER3 in adipocyte precursor cells.
Snippet: Full length Per3 cDNA was subcloned into a pEF6/V5-His expression plasmid (Life Technologies).pCMV6-Bmal1-Myc construct was purchased from Origene
Showing 1-5 of 5 papers.
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