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Dnajc2 (NM_009584) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209498
Dnajc2 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 2 (Dnajc2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $579.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajc2
Synonyms AU020218; MIDA1; Zrf1; Zrf2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209498 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTCCTGCCGAGCGCCGCGGAGGGCCAGGGCACCGCCATCACCCACGCTCTGACCTCCGCCTCTT
CAGTCTGTCAAGTTGAACCTGTGGGAAGATGGTTTGAAGCTTTTGTTAAGAGGAGAAACAGAAATGCTTC
TACATCTTTTCAGGAACTGGAAGATAAGAAAGAGTTGTCAGAGGAATCAGAAGATGAAGAGTTACAGTTA
GAAGAATTCCCCATGCTGAAAACACTTGATCCCAAAGATTGGAAGAATCAAGATCATTATGCGGTTCTTG
GACTTGGCCACGTCAGATACACAGCTACACAGAGACAGATCAAAGCAGCTCATAAAGCAATGGTCTTGAA
ACATCATCCAGACAAACGGAAAGCGGCTGGGGAACCCATCAAAGAAGGAGACAATGACTACTTCACTTGC
ATAACTAAAGCTTATGAAATGTTGTCTGATCCAGTGAAAAGGCGGGCATTTAACAGTGTAGATCCTACTT
TTGACAACTCAGTTCCTTCTAAAAGTGAAGCTAAGGACAATTTTTTTCAAGTGTTTTCTCCAGTGTTTGA
AAGGAATTCAAGATGGTCAAATAAAAAAAATGTTCCCAAGCTTGGTGATATGAACTCCTCGTTTGAAGAT
GTAGATGCGTTTTACTCTTTCTGGTATAATTTTGATTCTTGGAGAGAATTTTCTTATTTAGATGAAGAAG
AAAAAGAAAAAGCAGAATGCCGTGATGAGAGGAAGTGGATTGAAAAGCAGAACAGAGCCACGAGGGCACA
AAGGAAAAAGGAAGAGATGAACAGAATAAGAACATTAGTTGATAATGCTTACAGTTGTGACCCAAGGATA
AAAAAATTTAAGGAAGAAGAAAAGGCCAAGAAAGAAGCAGAAAAGAAAGCAAAAGCAGAGGCCAGGCGGA
AGGAGCAGGAAGCCAAAGAAAAGCAAAGACAAGCTGAATTAGAAGCTGTTCGGTTGGCTAAGGAGAAAGA
AGAGGAAGAAGTTAGACAGCAAGCATTGTTGGCCAAGAAGGAAAAAGATATCCAGAAGAAAGCCATTAAA
AAGGAAAGGCAGAAACTTCGGAACTCGTGCAAGAGCTGGAATCACTTCTCGGACAATGAGGCAGACCGTG
TTAAAATGATGGAAGAGGTGGAAAAACTCTGTGATCGGCTTGAACTAGCAAGCTTGCAGGGCTTGAATGA
AATCCTGGCTTCATCTACGAGAGAAGTAGGAAAAGCTGCACTTGAAAAACAGATAGAAGAAGTGAATGAG
CAGATGAGGAGAGAGAAGGAGGAAGCTGATGCTCGGATGCGGCAAGCATCCAAGAATGCAGAGAAGTCAA
CTGGTGGAAGTGGGAGTGGCAGTAAAAATTGGTCAGAAGATGATCTGCAATTATTAATTAAAGCTGTCAA
TCTCTTCCCTGCTGGAACAAACTCAAGATGGGAAGTCATTGCTAATTATATGAACATACATTCTTCTTCT
GGCGTCAAAAGAACTGCCAAAGATGTTATTAGCAAGGCTAAAAGCCTCCAAAAACTTGACCCTCATCAAA
AAGATGACATTAACAAAAAGGCTTTTGATAAGTTTAAAAAAGAACATGGAGTGGCCTCTCAGGCTGACAG
TGCAGCACCATCAGAACGGTTTGAAGGTCCCTGCATAGATTCCACCCCTTGGACAACAGAAGAACAGAAA
CTTTTAGAACAGGCTTTGAAAACATACCCAGTAAATACTCCTGAACGATGGGAAAAGATAGCCGAAGCTG
TTCCTGGCAGGACGAAGAAGGACTGCATGAGGCGATACAAGGAACTTGTGGAGATGGTGAAAGCCAAGAA
AGCTGCTCAGGAGCAAGTGCTGAATGCAAGCAGAGCCAGGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209498 protein sequence
Red=Cloning site Green=Tags(s)

MLLLPSAAEGQGTAITHALTSASSVCQVEPVGRWFEAFVKRRNRNASTSFQELEDKKELSEESEDEELQL
EEFPMLKTLDPKDWKNQDHYAVLGLGHVRYTATQRQIKAAHKAMVLKHHPDKRKAAGEPIKEGDNDYFTC
ITKAYEMLSDPVKRRAFNSVDPTFDNSVPSKSEAKDNFFQVFSPVFERNSRWSNKKNVPKLGDMNSSFED
VDAFYSFWYNFDSWREFSYLDEEEKEKAECRDERKWIEKQNRATRAQRKKEEMNRIRTLVDNAYSCDPRI
KKFKEEEKAKKEAEKKAKAEARRKEQEAKEKQRQAELEAVRLAKEKEEEEVRQQALLAKKEKDIQKKAIK
KERQKLRNSCKSWNHFSDNEADRVKMMEEVEKLCDRLELASLQGLNEILASSTREVGKAALEKQIEEVNE
QMRREKEEADARMRQASKNAEKSTGGSGSGSKNWSEDDLQLLIKAVNLFPAGTNSRWEVIANYMNIHSSS
GVKRTAKDVISKAKSLQKLDPHQKDDINKKAFDKFKKEHGVASQADSAAPSERFEGPCIDSTPWTTEEQK
LLEQALKTYPVNTPERWEKIAEAVPGRTKKDCMRRYKELVEMVKAKKAAQEQVLNASRARK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009584
ORF Size 1863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009584.4
RefSeq Size 2037 bp
RefSeq ORF 1866 bp
Locus ID 22791
UniProt ID P54103
Cytogenetics 5 9.97 cM
MW 71.7 kDa
Summary Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation (By similarity). Specifically binds DNA sequence 5'-GTCAAGC-3'.UniProtKB/Swiss-Prot Function
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"NM_009584" in other vectors (4)
SKU Description Size Price
MC219814 Dnajc2 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 2 (Dnajc2), (10ug) 10 ug
$579.00
MG209498 Dnajc2 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 2 (Dnajc2) 10 ug
$779.00
MR209498L3 Lenti ORF clone of Dnajc2 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 2 (Dnajc2) 10 ug
$879.00
MR209498L4 Lenti ORF clone of Dnajc2 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 2 (Dnajc2) 10 ug
$879.00

Plasmid Map for MR209498

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209498
Circular map for MR209498

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