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Glis1 (BC066157) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209472
Glis1 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $575.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Glis1
Synonyms Gli6, GliH1, Gli5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209472 representing BC066157
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCAGCGCCCACACACCCCTGCCCACCCACTGCCGAGCCCCATCGTCCATGGGTCTGCCCTCAGACC
TGGACTTTCCAGACCGAGGCCTCACCAACCCTGCACCTTCCTGCTACCTTCTGGGCAATGAACCCATCTC
AGACCTGGGTCCCCAACCCGAGGCCCACCTCCCCGAGGGCAGCCTGAAACGCTGCTGCCTCCTGGGCCTG
CCCCCCACCTCTTCAGCCTCCTCCTCACCCTGTGCCTCCTCAGATATCAATCCTGTCATCCACTCCTCCC
AGACAGCTCTAGTTAGCTGTGTAAATGGACTCCGAAGCCCACCTCTGCCGGGAGACCTGGGGGGCCCTCC
CAAGCGGTCACGGCCCGGGCCTGCATCCAGTGACGGCCAGGAGGGCAGCTTGCAGCTTGAAGCATGCCGG
AAGTCAGGCTTCCTGAAGCAGGAGCCCATGGACGAGTTTTCAGAGCTTTTTGCTCCACACCACCAGGGTT
TGCCACCCCCTTACCCCTTGCCTCAGTTGCCAACTGGCCCCGGCCTCGGAGGCCTAGGGCTGGGCCTGGC
AGGTAGGATGGTTGCCGGTCGGCAGGCATGCCGCTGGGTGGACTGCTGCGCAGCCTACGGGCAGCAGGAG
GAGCTGGAGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAAGACTTCACCTGCTTCTGGG
CCGGGTGTGTGCGGCGCTACAAGCCCTTCAATGCCCGCTACAAGCTGCTCATCCACATGAGGGTACACTC
AGGCGAGAAGCCCAACAAGTGCATGTTCGAAGGCTGCAGTAAAGCCTTTTCCCGTCTGGAGAACCTGAAG
ATCCATCTGCGGAGCCACACAGGCGAGAAACCATACCTGTGCCAGCACCCAGGCTGCCAGAAGGCCTTCA
GCAACTCCAGCGACCGTGCCAAGCACCAACGCACCCACCTCGACACGAAGCCATATGCTTGTCAGATCCC
TGGCTGCTCCAAGCGCTACACGGACCCCAGCTCCCTCCGCAAGCACGTGAAGGCCCACTCAGCCAAAGAG
CAGCAGGTGCGTAAGAAGCTGCACACAGGTGCCGACCCAGAGGCTGATGTTCTGTCCGAGTGTCTGTCCC
TGCAGCAGCTCCAAGCATCCACACTGTTGCCGGCCAGCAGAGGGAAGGGCAGCCAAACCCTGAGCCAGGA
GCTCCTCCCAGGTGTGTATCCTGGCTCCGTCACCCCACAAAACGGGCTTGCTTCAGGCATCCTGTCCCCC
TCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACTGGTTCCCACCACCACCTGTCCCCTC
TGCCCACAGCTGAGAGCACCAGGGATGGCCTGGGGCCCAGTCTCCTTTCACCCATGGCCAGCCCACTGAA
GGGGCTTGGTCCCCCACCGCTACCACCAGCCTCCCAGAGTCAGTCTCCAGGGGGACAGTCATTCTCTACA
GTCCCCAGCAAGCCTACCTCCCCATCCTTCCAAAGCCCACCACCTCTGCCCAGCCCCCAAGGCTACCAAG
GCAGTTTCCATTCCATCCAGAACTGCTTCCCCTACGCTGACTGCTACCGGGCCACTGAGCCAGCAGCCTC
CAGGGATGGACTGGTGGGTGATGCCCACGGTTTCAACCCCTTGCGACCCAGCACATACTCCAGCCTCAGC
ACACCTTTATCCGCACCAGGCTACGAGACCCTGGCAGAAACGCCGTGTCCCCCAGCGCTGCAGCCACAGC
CAGCTGAAGACCTGGTACCTAGTGGTCCTGAGGACTGTGGCTTCTTCCCCAATGGGGCCTTTGACCACTG
TCTGAGTCACATCCCGTCCATCTACACTGACACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209472 representing BC066157
Red=Cloning site Green=Tags(s)

MVSAHTPLPTHCRAPSSMGLPSDLDFPDRGLTNPAPSCYLLGNEPISDLGPQPEAHLPEGSLKRCCLLGL
PPTSSASSSPCASSDINPVIHSSQTALVSCVNGLRSPPLPGDLGGPPKRSRPGPASSDGQEGSLQLEACR
KSGFLKQEPMDEFSELFAPHHQGLPPPYPLPQLPTGPGLGGLGLGLAGRMVAGRQACRWVDCCAAYGQQE
ELERHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLK
IHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKE
QQVRKKLHTGADPEADVLSECLSLQQLQASTLLPASRGKGSQTLSQELLPGVYPGSVTPQNGLASGILSP
SHDVPSRHHPLEVPTGSHHHLSPLPTAESTRDGLGPSLLSPMASPLKGLGPPPLPPASQSQSPGGQSFST
VPSKPTSPSFQSPPPLPSPQGYQGSFHSIQNCFPYADCYRATEPAASRDGLVGDAHGFNPLRPSTYSSLS
TPLSAPGYETLAETPCPPALQPQPAEDLVPSGPEDCGFFPNGAFDHCLSHIPSIYTDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC066157
ORF Size 1854 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC066157.1
RefSeq Size 2431 bp
RefSeq ORF 1856 bp
Locus ID 230587
Cytogenetics 4 C7
MW 89.1 kDa
Summary Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).UniProtKB/Swiss-Prot Function
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"BC066157" in other vectors (4)
SKU Description Size Price
MC218609 Glis1 (untagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539), (10ug) 10 ug
$576.00
MG209472 Glis1 (tGFP-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) 10 ug
$775.00
MR209472L3 Lenti ORF clone of Glis1 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) 10 ug
$875.00
MR209472L4 Lenti ORF clone of Glis1 (mGFP-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) 10 ug
$875.00

Plasmid Map for MR209472

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209472
Circular map for MR209472

file-alt Citations

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