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Glis1 (BC066157) Mouse Untagged Clone

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SKU
MC218609
Glis1 (untagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $576.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Glis1
Synonyms Gli6, GliH1, Gli5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC218609 representing BC066157
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCAGCGCCCACACACCCCTGCCCACCCACTGCCGAGCCCCATCGTCCATGGGTCTGCCCTCAGACC
TGGACTTTCCAGACCGAGGCCTCACCAACCCTGCACCTTCCTGCTACCTTCTGGGCAATGAACCCATCTC
AGACCTGGGTCCCCAACCCGAGGCCCACCTCCCCGAGGGCAGCCTGAAACGCTGCTGCCTCCTGGGCCTG
CCCCCCACCTCTTCAGCCTCCTCCTCACCCTGTGCCTCCTCAGATATCAATCCTGTCATCCACTCCTCCC
AGACAGCTCTAGTTAGCTGTGTAAATGGACTCCGAAGCCCACCTCTGCCGGGAGACCTGGGGGGCCCTCC
CAAGCGGTCACGGCCCGGGCCTGCATCCAGTGACGGCCAGGAGGGCAGCTTGCAGCTTGAAGCATGCCGG
AAGTCAGGCTTCCTGAAGCAGGAGCCCATGGACGAGTTTTCAGAGCTTTTTGCTCCACACCACCAGGGTT
TGCCACCCCCTTACCCCTTGCCTCAGTTGCCAACTGGCCCCGGCCTCGGAGGCCTAGGGCTGGGCCTGGC
AGGTAGGATGGTTGCCGGTCGGCAGGCATGCCGCTGGGTGGACTGCTGCGCAGCCTACGGGCAGCAGGAG
GAGCTGGAGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAAGACTTCACCTGCTTCTGGG
CCGGGTGTGTGCGGCGCTACAAGCCCTTCAATGCCCGCTACAAGCTGCTCATCCACATGAGGGTACACTC
AGGCGAGAAGCCCAACAAGTGCATGTTCGAAGGCTGCAGTAAAGCCTTTTCCCGTCTGGAGAACCTGAAG
ATCCATCTGCGGAGCCACACAGGCGAGAAACCATACCTGTGCCAGCACCCAGGCTGCCAGAAGGCCTTCA
GCAACTCCAGCGACCGTGCCAAGCACCAACGCACCCACCTCGACACGAAGCCATATGCTTGTCAGATCCC
TGGCTGCTCCAAGCGCTACACGGACCCCAGCTCCCTCCGCAAGCACGTGAAGGCCCACTCAGCCAAAGAG
CAGCAGGTGCGTAAGAAGCTGCACACAGGTGCCGACCCAGAGGCTGATGTTCTGTCCGAGTGTCTGTCCC
TGCAGCAGCTCCAAGCATCCACACTGTTGCCGGCCAGCAGAGGGAAGGGCAGCCAAACCCTGAGCCAGGA
GCTCCTCCCAGGTGTGTATCCTGGCTCCGTCACCCCACAAAACGGGCTTGCTTCAGGCATCCTGTCCCCC
TCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACTGGTTCCCACCACCACCTGTCCCCTC
TGCCCACAGCTGAGAGCACCAGGGATGGCCTGGGGCCCAGTCTCCTTTCACCCATGGCCAGCCCACTGAA
GGGGCTTGGTCCCCCACCGCTACCACCAGCCTCCCAGAGTCAGTCTCCAGGGGGACAGTCATTCTCTACA
GTCCCCAGCAAGCCTACCTCCCCATCCTTCCAAAGCCCACCACCTCTGCCCAGCCCCCAAGGCTACCAAG
GCAGTTTCCATTCCATCCAGAACTGCTTCCCCTACGCTGACTGCTACCGGGCCACTGAGCCAGCAGCCTC
CAGGGATGGACTGGTGGGTGATGCCCACGGTTTCAACCCCTTGCGACCCAGCACATACTCCAGCCTCAGC
ACACCTTTATCCGCACCAGGCTACGAGACCCTGGCAGAAACGCCGTGTCCCCCAGCGCTGCAGCCACAGC
CAGCTGAAGACCTGGTACCTAGTGGTCCTGAGGACTGTGGCTTCTTCCCCAATGGGGCCTTTGACCACTG
TCTGAGTCACATCCCGTCCATCTACACTGACACCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC066157
Insert Size 1857 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC066157, AAH66157
RefSeq Size 2431 bp
RefSeq ORF 1856 bp
Locus ID 230587
Cytogenetics 4 C7
Summary Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).UniProtKB/Swiss-Prot Function
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"BC066157" in other vectors (4)
SKU Description Size Price
MG209472 Glis1 (tGFP-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) 10 ug
$775.00
MR209472 Glis1 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) 10 ug
$289.00 $575.00
MR209472L3 Lenti ORF clone of Glis1 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) 10 ug
$875.00
MR209472L4 Lenti ORF clone of Glis1 (mGFP-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) 10 ug
$875.00

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