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Ppm1d (BC023492) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209279
Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $556.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppm1d
Synonyms AV338790; Wip1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209279 representing BC023492
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCGGACCAGGGCGGGAGGAAGTACATGGAGGACG
TAACTCAGATCGTGGTGGAGCCCGAGCCGGCTGCGGAGGACAAGCCGGCGCCGGTACCGCGGCGGGCGCT
CGGGTTGCCGGCGACCCCCACTCTCGCCGGCGTCGGGCCATCGGAAAAAGGCCCGGCGGCGGCCCGCGAC
CCTGCCCCGGACGCCGCGGCCTCGCTACCCGCTGGCCGCTGCTGTCGCCGCCGCTCTTCGGTGGCCTTCT
TTGCAGTGTGCGACGGGCACGGCGGTCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTGTGGGGTTTCAT
CAAGAAGCAGAAAGGCTTCACCTCGTCCGAGCCCGCCAAGGTGTGCGCTGCCATCCGCAAAGGTTTCCTC
GCCTGTCACCTCGCCATGTGGAAGAAACTGGAATGGCCAAAGACTATGACAGGTCTTCCCAGCACGTCCG
GGACAACTGCCAGTGTGGTTATAATACGGGGCATGAAGATGTATGTAGCGCATGTAGGTGACTCTGGGGT
GGTCCTTGGAATTCAGGATGACCCAAAGGATGATTTTGTGAGAGCTGTGGAGGTGACACAAGATCACAAG
CCAGAACTTCCCAAGGAAAGAGAAAGAATCGAAGGACTTGGCGGCAGTGTGATGAACAAGTCTGGAGTGA
ACCGAGTAGTTTGGAAAAGGCCCCGGCTCACTCACAGTGGACCTGTCAGAAGGAGCACAGTCATTGACCA
GATTCCTTTTCTGGCTGTAGCAAGAGCGCTTGGTGACTTGTGGAGCTATGATTTCTTCAGTGGTAAGTTT
GTGGTGTCACCTGAACCAGACACCAGTGTCCACACTCTTGACCCCCGGAAGCACAAGTATATTATCCTGG
GAAGTGATGGACTTTGGAATATGGTTCCACCTCAAGATGCCATCTCCATGTGCCAAGACCAAGAGGAGAA
AAAATACTTGATGGGTGAGCAAGGACAGTCCTGTGCCAAAATGCTTGTGAATCGAGCACTTGGCCGCTGG
AGGCAGCGTATGCTTCGGGCAGATAACACAAGTGCCATCGTAATCTGCATCTCTCCAGAAGTAGACAACC
AAGGGAACTTCACCAATGAAGATGAGCTCTTTCTGAACCTGACTGATAGCCCTACTTACAACAGCCAGGA
GACCTGTGTGATGACTTCTTCTCCAAGTTCTACACCACCAATCAAGTCACCGGAAGAAGATGCATGGCCA
AGGCTGAGCTCTAAGGACCATATACCTGCCCTTGTTCGCAGTAATGCCTTCTCAGAGAAGTTTTTAGAGG
TCCCAGCTGAGATAGCTAGAGGGAATATCCAGACTGTAGTGATGACCTCAAAAGACTCAGAGACACTTGA
AGAAAATTGCCCCAAAGCCCTGACTTTAAGGATTCATGATTCTTTGAATAATACTCTGTCAGTTGGCCTC
ATTCCAACCAATTCAACAAATACTATCATGGACCAAAAAAACTTAAAGATGTCAACTCCAGGTCAAATGA
AAGCTCAAGAAGTTGAAAGAACCCCTCCAGCCAATTTTAAAAGGACATTAGAAGAATCCAACTCTGGCCC
CCTTATGAAGAAGCACCGACGAAATGGCTTAAGTCGAAGTAGCGGGGCCCAGGCTTCCAGTCTCCCTACA
GCATCCCAGCGCAGGCACTCTGTCAAACTGACCCTGAGGCGCAGACTCAGGGGCCAGAGGAAGATGGGAA
ATCCTCTTCTCCACCAGCACCGGAAAACAGTGTGTGTGTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209279 representing BC023492
Red=Cloning site Green=Tags(s)

MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPAAEDKPAPVPRRALGLPATPTLAGVGPSEKGPAAARD
PAPDAAASLPAGRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFL
ACHLAMWKKLEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHK
PELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHSGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGKF
VVSPEPDTSVHTLDPRKHKYIILGSDGLWNMVPPQDAISMCQDQEEKKYLMGEQGQSCAKMLVNRALGRW
RQRMLRADNTSAIVICISPEVDNQGNFTNEDELFLNLTDSPTYNSQETCVMTSSPSSTPPIKSPEEDAWP
RLSSKDHIPALVRSNAFSEKFLEVPAEIARGNIQTVVMTSKDSETLEENCPKALTLRIHDSLNNTLSVGL
IPTNSTNTIMDQKNLKMSTPGQMKAQEVERTPPANFKRTLEESNSGPLMKKHRRNGLSRSSGAQASSLPT
ASQRRHSVKLTLRRRLRGQRKMGNPLLHQHRKTVCVC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC023492
ORF Size 1791 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC023492.1
RefSeq Size 2023 bp
RefSeq ORF 1793 bp
Locus ID 53892
Cytogenetics 11 51.34 cM
MW 74.1 kDa
Summary Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (By similarity). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (PubMed:24135283).UniProtKB/Swiss-Prot Function
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"BC023492" in other vectors (3)
SKU Description Size Price
MG209279 Ppm1d (tGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) 10 ug
$756.00
MR209279L3 Lenti ORF clone of Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) 10 ug
$856.00
MR209279L4 Lenti ORF clone of Ppm1d (mGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) 10 ug
$856.00

Plasmid Map for MR209279

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209279
Circular map for MR209279

file-alt Citations

Wip1 phosphatase modulates both long-term potentiation and long-term depression through the dephosphorylation of CaMKII
He, Hu, Zhang et al
Cell Adh Migr (2016) 10 (3), 237-47 DOI: 10.4161/19336918.2014.994916
AI Summary: OriGene products, specifically the Wip1 and CaMKIIβ constructs, were used to investigate the roles of these proteins in modulating synaptic plasticity in the study.
Snippet: The Wip1 and CaMKIIβ constructs were purchased from Origene
Showing 1-1 of 1 papers.
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