Ppm1d (BC023492) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG209279
Ppm1d (tGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376)
$756.00
3 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ppm1d |
| Synonyms | AV338790; Wip1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC023492 |
| ORF Size | 1791 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC023492.1 |
| RefSeq Size | 2023 bp |
| RefSeq ORF | 1793 bp |
| Locus ID | 53892 |
| Cytogenetics | 11 51.34 cM |
| Summary | Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (By similarity). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (PubMed:24135283).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"BC023492" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR209279 | Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) | 10 ug |
$289.00
$556.00
|
|
| MR209279L3 | Lenti ORF clone of Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) | 10 ug |
$856.00
|
|
| MR209279L4 | Lenti ORF clone of Ppm1d (mGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376) | 10 ug |
$856.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
