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Parp2 (NM_009632) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208862
Parp2 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 2 (Parp2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $520.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Parp2
Synonyms Adprt2; Adprtl2; ARTD2; Aspartl2; C78626; PARP-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208862 representing NM_009632
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGCGGCGGCAGAGATCAGGCTCTGGAAGGCGAGTGCTAAATGAAGCCAAGAAAGTTGATAATG
GCAACAAAGCAACAGAAGACGACTCTCCTCCTGGCAAGAAGATGCGCACGTGCCAGAGAAAAGGGCCTAT
GGCTGGAGGGAAGGACGCAGACAGGACAAAAGACAATCGAGACTCTGTGAAGACCTTGCTGTTAAAGGGC
AAAGCCCCTGTGGACCCAGAGTGTGCAGCCAAGCTGGGAAAGGCTCATGTGTATTGTGAAGGAGATGATG
TCTATGATGTCATGCTAAATCAAACCAATCTCCAGTTCAACAACAACAAGTACTACCTTATTCAGCTGTT
AGAAGATGATGCCCAGAGGAACTTCAGTGTTTGGATGAGGTGGGGCCGAGTTGGAAAGACGGGGCAGCAC
AGCTTGGTGACTTGTTCTGGTGACCTCAACAAAGCAAAAGAAATATTTCAGAAAAAATTCCTTGACAAAA
CTAAAAACAATTGGGAGGACCGTGAGAACTTTGAAAAAGTACCTGGAAAATACGACATGTTACAGATGGA
CTATGCTGCCAGCACGCAGGATGAAAGTAAAACAAAAGAAGAGGAAACTTTGAAGCCTGAGTCTCAGCTG
GATCTTCGAGTCCAGGAGCTGCTAAAGTTGATCTGTAACGTGCAGACCATGGAAGAAATGATGATTGAGA
TGAAGTATGACACCAAGAGAGCCCCGCTTGGAAAGCTGACAGTGGCGCAAATCAAGGCCGGTTACCAGTC
TCTCAAGAAGATTGAGGACTGCATCCGCGCTGGCCAGCATGGGCGAGCGCTTGTTGAAGCGTGCAATGAA
TTCTACACCAGGATCCCTCATGACTTTGGACTCTCCATCCCTCCAGTAATCCGGACAGAGAAGGAACTGT
CAGACAAAGTAAAACTGCTAGAGGCATTGGGAGACATTGAAATTGCCCTTAAACTGGTGAAGTCAGAGCG
CCAAGGCCTAGAACACCCACTGGACCAACACTATAGAAACCTACACTGTGCTTTGCGTCCTCTGGACCAT
GAAAGTAATGAGTTTAAGGTGATTTCTCAGTACCTACAGTCTACGCATGCTCCTACACACAAGGACTATA
CTATGACCTTGCTGGATGTTTTCGAAGTAGAGAAGGAAGGGGAGAAAGAGGCCTTCAGGGAGGACCTTCC
TAACAGGATGCTGCTCTGGCATGGATCCAGGCTGAGTAACTGGGTGGGGATCCTGAGCCACGGGCTTAGA
GTTGCCCCACCTGAGGCTCCCATCACAGGTTATATGTTTGGAAAAGGAATCTACTTTGCTGACATGTCCT
CCAAGAGTGCCAATTACTGCTTTGCCTCTCGCCTAAAGAATACAGGATTGCTTCTTCTGTCAGAGGTAGC
TCTAGGTCAGTGTAATGAACTACTGGAGGCCAATCCTAAAGCACAAGGATTGCTTCGGGGCAAGCATAGC
ACCAAGGGGATGGGAAAGATGGCTCCCAGCCCTGCCCACTTCATCACCCTGAATGGGAGTACAGTGCCCT
TAGGACCAGCAAGTGACACAGGAATTCTCAATCCAGAGGGGTACACCCTCAACTACAATGAGTTTATTGT
TTATAGCCCCAACCAGGTCCGTATGCGATACCTTCTAAAGATTCAATTTAACTTCCTGCAGCTATGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208862 representing NM_009632
Red=Cloning site Green=Tags(s)

MAPRRQRSGSGRRVLNEAKKVDNGNKATEDDSPPGKKMRTCQRKGPMAGGKDADRTKDNRDSVKTLLLKG
KAPVDPECAAKLGKAHVYCEGDDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKTGQH
SLVTCSGDLNKAKEIFQKKFLDKTKNNWEDRENFEKVPGKYDMLQMDYAASTQDESKTKEEETLKPESQL
DLRVQELLKLICNVQTMEEMMIEMKYDTKRAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALVEACNE
FYTRIPHDFGLSIPPVIRTEKELSDKVKLLEALGDIEIALKLVKSERQGLEHPLDQHYRNLHCALRPLDH
ESNEFKVISQYLQSTHAPTHKDYTMTLLDVFEVEKEGEKEAFREDLPNRMLLWHGSRLSNWVGILSHGLR
VAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAQGLLRGKHS
TKGMGKMAPSPAHFITLNGSTVPLGPASDTGILNPEGYTLNYNEFIVYSPNQVRMRYLLKIQFNFLQLW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009632
ORF Size 1677 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009632.3
RefSeq Size 1834 bp
RefSeq ORF 1680 bp
Locus ID 11546
UniProt ID O88554
Cytogenetics 14 C1
MW 63.8 kDa
Summary Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231). Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (By similarity). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:10364231). Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (By similarity). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009632" in other vectors (4)
SKU Description Size Price
MC218931 Parp2 (untagged) - Mouse poly (ADP-ribose) polymerase family, member 2 (Parp2), (10ug) 10 ug
$573.00
MG208862 Parp2 (tGFP-tagged) - Mouse poly (ADP-ribose) polymerase family, member 2 (Parp2) 10 ug
$720.00
MR208862L3 Lenti ORF clone of Parp2 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 2 (Parp2) 10 ug
$820.00
MR208862L4 Lenti ORF clone of Parp2 (mGFP-tagged) - Mouse poly (ADP-ribose) polymerase family, member 2 (Parp2) 10 ug
$820.00

Plasmid Map for MR208862

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208862
Circular map for MR208862

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