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Iws1 (BC068184) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208664
Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $505.00
In Stock*
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Other products for "Iws1 or BC068184"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Iws1
Synonyms 1700069O15Rik; 3000008H23; AL117927; AW214353
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208664 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCGGAGTACTACAGCGGGGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG
ATTCAGGGTCAGATGGTGAGGATGGTGTAACCGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCA
CCACTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGGCCAAGATACATAATGGAACAGACTCTGAG
AATGATGAACCTTCCAATGCTAATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAGGACAGTG
ACTCTGACTCTGAGGAGCATGCAGAGTCTCCTGCTAGTGATTCTGAAAATGAACCTGTCAACCAACATGG
GAGCGACTCTGAAAATGAAGAGCTGCTTAATGGGCATGCCAGTGACTCTGAAAAGGAAGAGGTTAGCAAG
CATGCCGCCAGTGACTCTGAAGCTGAGGACACTCTGCAGCCTCAGGTCAGCGAGTCTGACAGTGAAGATC
CCCCAAGGCCCCAGGCCAGTGACTCAGAAAATGAGGAGCCTCCCAAACCTCGGATAAGTGACTCGGAGAG
TGAGGAGCTCCCCAAACCCCGAGTCAGTGACTCAGAAAGTGAGGACCCTCCAAGGCCCCAGGCCAGTGAC
TCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCTGAAAGTGAGGACCCTCCAAGGCCCCAGG
CCAGTGACTCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA
AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACACAAAGAGAAGCCAGACTCAGATGACAGT
GATGGGGAGAATAAGAGGGAAGATTCTGAAGTGCAGAATGAGTCAGATGGCCATACAGACAGAAAAGGAC
TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAAAGGCAAAAAATTGACAGTGATGATGATGAAGAAAA
GGAGGGGGATGAGGAGAAAGTAGCAAAGCGGAAGGCTGCTGTGCTTTCTGATAGTGAGGATGACGCAGGC
AACGCATCAGCAAAGAAGAGTCGAGTTGTCTGTGATGCTGATGACTCTGATAGTGATGTTGTATCGGACA
AGTCAGGCAAACGAGAGACAACAGTAGCATCTGACAGTGAAGAAGAAGCCGGGAAAGAAGAGTCTTCTGT
GAAGAAGAAGGAAGACAAGGACCTGTTTGGGAGTGACAGTGAGTCCGGCAATGAAGAAGAAAATCTTATT
GCAGATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACGGGTTTTAACCAAGAAGATTTGG
AGGAAGAGAAAAACGAAACACAGCTAAAGGAAGCAGAAGATTCTGATTCTGATGATAACATAAAGAGAGG
CAAGCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAAAGCATGTGTGGCAAACGC
AGACGAAATCGAGATGGGGGCACTTTTATTAGTGATGCAGATGATGTTGTGAGCGCCATGATTGTCAAGA
TGAATGAAGCTGCTGAGGTAAGCTCTTCTAGGTTACCCTTCTCCCCTCCTGCCCTCAACCAAATTGATGG
GGTGGCAAATCTTTCTCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208664 protein sequence
Red=Cloning site Green=Tags(s)

MDSEYYSGDQSDDGGATPVQDERDSGSDGEDGVTEQHSGSDTGSVDHHSENETSDREDGLAKIHNGTDSE
NDEPSNANASDSESEELHRPKDSDSDSEEHAESPASDSENEPVNQHGSDSENEELLNGHASDSEKEEVSK
HAASDSEAEDTLQPQVSESDSEDPPRPQASDSENEEPPKPRISDSESEELPKPRVSDSESEDPPRPQASD
SESEELPKPRVSDSESEDPPRPQASDSESEELPKPRVSDSESEDPQKGPASDSEAEDASRHKEKPDSDDS
DGENKREDSEVQNESDGHTDRKGLHSSDSEEEEPKRQKIDSDDDEEKEGDEEKVAKRKAAVLSDSEDDAG
NASAKKSRVVCDADDSDSDVVSDKSGKRETTVASDSEEEAGKEESSVKKKEDKDLFGSDSESGNEEENLI
ADIFGESGDEEEEEFTGFNQEDLEEEKNETQLKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMCGKR
RRNRDGGTFISDADDVVSAMIVKMNEAAEVSSSRLPFSPPALNQIDGVANLSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC068184
ORF Size 1629 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC068184, AAH68184
RefSeq Size 4353 bp
RefSeq ORF 1631 bp
Locus ID 73473
Cytogenetics 18 B1
MW 59.3 kDa
Summary Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).UniProtKB/Swiss-Prot Function
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"BC068184" in other vectors (4)
SKU Description Size Price
MC218462 Iws1 (untagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737), (10ug) 10 ug
$557.00
MG208664 Iws1 (tGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) 10 ug
$705.00
MR208664L3 Lenti ORF clone of Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) 10 ug
$805.00
MR208664L4 Lenti ORF clone of Iws1 (mGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) 10 ug
$805.00

Plasmid Map for MR208664

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208664
Circular map for MR208664

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