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Iws1 (BC068184) Mouse Untagged Clone

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SKU
MC218462
Iws1 (untagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $557.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Iws1
Synonyms 1700069O15Rik; 3000008H23; AL117927; AW214353
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC068184
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCGGAGTACTACAGCGGGGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG
ATTCAGGGTCAGATGGTGAGGATGGTGTAACCGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCA
CCACTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGGCCAAGATACATAATGGAACAGACTCTGAG
AATGATGAACCTTCCAATGCTAATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAGGACAGTG
ACTCTGACTCTGAGGAGCATGCAGAGTCTCCTGCTAGTGATTCTGAAAATGAACCTGTCAACCAACATGG
GAGCGACTCTGAAAATGAAGAGCTGCTTAATGGGCATGCCAGTGACTCTGAAAAGGAAGAGGTTAGCAAG
CATGCCGCCAGTGACTCTGAAGCTGAGGACACTCTGCAGCCTCAGGTCAGCGAGTCTGACAGTGAAGATC
CCCCAAGGCCCCAGGCCAGTGACTCAGAAAATGAGGAGCCTCCCAAACCTCGGATAAGTGACTCGGAGAG
TGAGGAGCTCCCCAAACCCCGAGTCAGTGACTCAGAAAGTGAGGACCCTCCAAGGCCCCAGGCCAGTGAC
TCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCTGAAAGTGAGGACCCTCCAAGGCCCCAGG
CCAGTGACTCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA
AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACACAAAGAGAAGCCAGACTCAGATGACAGT
GATGGGGAGAATAAGAGGGAAGATTCTGAAGTGCAGAATGAGTCAGATGGCCATACAGACAGAAAAGGAC
TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAAAGGCAAAAAATTGACAGTGATGATGATGAAGAAAA
GGAGGGGGATGAGGAGAAAGTAGCAAAGCGGAAGGCTGCTGTGCTTTCTGATAGTGAGGATGACGCAGGC
AACGCATCAGCAAAGAAGAGTCGAGTTGTCTGTGATGCTGATGACTCTGATAGTGATGTTGTATCGGACA
AGTCAGGCAAACGAGAGACAACAGTAGCATCTGACAGTGAAGAAGAAGCCGGGAAAGAAGAGTCTTCTGT
GAAGAAGAAGGAAGACAAGGACCTGTTTGGGAGTGACAGTGAGTCCGGCAATGAAGAAGAAAATCTTATT
GCAGATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACGGGTTTTAACCAAGAAGATTTGG
AGGAAGAGAAAAACGAAACACAGCTAAAGGAAGCAGAAGATTCTGATTCTGATGATAACATAAAGAGAGG
CAAGCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAAAGCATGTGTGGCAAACGC
AGACGAAATCGAGATGGGGGCACTTTTATTAGTGATGCAGATGATGTTGTGAGCGCCATGATTGTCAAGA
TGAATGAAGCTGCTGAGGTAAGCTCTTCTAGGTTACCCTTCTCCCCTCCTGCCCTCAACCAAATTGATGG
GGTGGCAAATCTTTCTCTTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC068184
Insert Size 1632 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC068184, AAH68184
RefSeq Size 4353 bp
RefSeq ORF 1631 bp
Locus ID 73473
Cytogenetics 18 B1
Summary Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).UniProtKB/Swiss-Prot Function
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"BC068184" in other vectors (4)
SKU Description Size Price
MG208664 Iws1 (tGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) 10 ug
$705.00
MR208664 Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) 10 ug
$289.00 $505.00
MR208664L3 Lenti ORF clone of Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) 10 ug
$805.00
MR208664L4 Lenti ORF clone of Iws1 (mGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) 10 ug
$805.00

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