Supt20 (NM_019995) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR208520
Supt20 (Myc-DDK-tagged) - Mouse family with sequence similarity 48, member A (Fam48a)
$289.00 $494.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Supt20
Synonyms AA667204; AI450544; D3Ertd300e; Fam48a; p38IP; Supt20h
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208520 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACAAGCTGTAGAACAAGCTTTGGATTGCGCAGAGTACATCGTGGAAAGTGCTCAGCAGAGACCTC
CTAAAAGGAAATACCTGTCTAGTGGAAGAAAGTCTATATTTCAAAAACTTTATGACTTATATGTTGAAGA
ATGTGAAAAAGAACCCGAGGTTAAGAAATTAAGAAGAAATGTGAACTTGTTAGAGAAGCTTGTTATGCAG
GAGACTTTGTCGTGTTTAGTAGTCAATCTGTACCCAGGAAATGAAGGCTATTCTCTGATGCTCAGGGGGA
AAAATGGATCAGATTCTGAGACCATCCGATTGCCTTATGAAGAAGGAGAATTGCTTGAATACTTAGATGC
AGAAGAATTACCTCCTATTTTGGTGGATCTCCTTGAAAAATCACAGGTTAATATTTTTCATTGTGGATGT
GTGATAGCAGAAATACGTGACTATAGGCAGTCCAGTAATATGAAGTCTCCTGGTTACCAAAGTAGGCACA
TTCTCTTACGTCCAACAATGCAGACTTTAGTCTGTGATGTCCATTCTATAACAAGTGACAACCATAAGTG
GACCCAGGAAGACAAACTTTTGCTTGAGAGCCAGCTGATTCTAGCTACAGCAGAGCCACTGTGTCTTGAC
CCATCGGTGGCAGTAGCGTGCACTGCCAATAGGCTGCTCTATAACCGGCAGAAGATGAACACTCGCCCAA
TGAAACGGTGTTGGAAGAGGTACTCCAGGTCCTCCCTGAACCGCCAGCAGGACCTCTCTCACGGCCCTCC
TCCCCCTCAGCTGAGACTACTTGATTTCTTACAAAAGCGGAAGGAAAGGAAAGCAGGCCAGCATTATGAC
CTCAAAATTTCCAAAGCAGGAAATTGTGTAGACATGTGGAAACGGAGCCCCTGTAACTTGGCTGTGCCTT
CTGAAGTAGATGTGGAAAAATATGCTAAAGTGGAGGAATCCATCAAATCTGACGACTCACAGCCAACAAT
GTGGCCAGCTCATGATGTAAAAGACGATTATGTATTTGAATGTGAAGGTGGTAATCAGTATCAGAAAACA
AAGCTGACCATCTTGCAGTCTCTTGGGGATCCACTTTACTATGGTAAAATACAGCCGTGGAAAGCAGATG
AAGAAAATGACAGCCAAATGTCTCCATCCCACTCCTCTGCTGATGACCATTCAAACTGGTTTGTCATTGG
GTCAAAGACTGACGCAGAGAGGGTAGTTAATCAATACCAGGAGTTGGTCCAGAATGAAGCCAAATGCCCA
GTGAAGATGTCACACAGCAGTAGTGGTTCAGCCGCTCTCAGTCCAGGGGAGGAGGCAGAACAGGCGGAGA
CCTCGTCCATTCAGTCTTCAGTTTTGGGAAAGGGAGTAAAACACCGACCCCCACCCATCAAACTTCCCTC
AGGCTCAGGAAATAGTTCCTCAGGTAACTATTTCACAGCACAGCAGGCCAGCAGCTTCCTTAAATCTCCA
ACTCCTCCTCCATCGTGTAAGCCAAGCCTTTCTCGGAAGTCTTCTGTGGACCTCAGTCAAGTTAGCATGC
TTTCTCCTGCCGCCCTGTCCCCTGCCAGCTCATCCCAGAGACATGAAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR208520 protein sequence
Red=Cloning site Green=Tags(s)

MQQAVEQALDCAEYIVESAQQRPPKRKYLSSGRKSIFQKLYDLYVEECEKEPEVKKLRRNVNLLEKLVMQ
ETLSCLVVNLYPGNEGYSLMLRGKNGSDSETIRLPYEEGELLEYLDAEELPPILVDLLEKSQVNIFHCGC
VIAEIRDYRQSSNMKSPGYQSRHILLRPTMQTLVCDVHSITSDNHKWTQEDKLLLESQLILATAEPLCLD
PSVAVACTANRLLYNRQKMNTRPMKRCWKRYSRSSLNRQQDLSHGPPPPQLRLLDFLQKRKERKAGQHYD
LKISKAGNCVDMWKRSPCNLAVPSEVDVEKYAKVEESIKSDDSQPTMWPAHDVKDDYVFECEGGNQYQKT
KLTILQSLGDPLYYGKIQPWKADEENDSQMSPSHSSADDHSNWFVIGSKTDAERVVNQYQELVQNEAKCP
VKMSHSSSGSAALSPGEEAEQAETSSIQSSVLGKGVKHRPPPIKLPSGSGNSSSGNYFTAQQASSFLKSP
TPPPSCKPSLSRKSSVDLSQVSMLSPAALSPASSSQRHES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019995
ORF Size 1590 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019995.1, NM_019995.2, NM_019995.3, NP_064379.1
RefSeq Size 1968 bp
RefSeq ORF 1596 bp
Locus ID 56790
UniProt ID Q7TT00
Cytogenetics 3 C
MW 59.5 kDa
Summary Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail. Required for starvation-induced ATG9A trafficking during autophagy.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019995" in other vectors (4)
SKU Description Size Price
MC217594 Supt20 (untagged) - Mouse family with sequence similarity 48, member A (Fam48a), (10ug) 10 ug
$545.00
MG208520 Supt20 (tGFP-tagged) - Mouse DNA segment, Chr 3, ERATO Doi 300, expressed (D3Ertd300e) 10 ug
$694.00
MR208520L3 Lenti ORF clone of Supt20 (Myc-DDK-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) 10 ug
$794.00
MR208520L4 Lenti ORF clone of Supt20 (mGFP-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) 10 ug
$794.00

Plasmid Map for MR208520

Download sequence for

Cloning scheme for MR208520

Circular map for MR208520

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