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Supt20 (NM_019995) Mouse Untagged Clone

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SKU
MC217594
Supt20 (untagged) - Mouse family with sequence similarity 48, member A (Fam48a), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $545.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Supt20
Synonyms AA667204; AI450544; D3Ertd300e; Fam48a; p38IP; Supt20h
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217594 representing NM_019995
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACAAGCTGTAGAACAAGCTTTGGATTGCGCAGAGTACATCGTGGAAAGTGCTCAGCAGAGACCTC
CTAAAAGGAAATACCTGTCTAGTGGAAGAAAGTCTATATTTCAAAAACTTTATGACTTATATGTTGAAGA
ATGTGAAAAAGAACCCGAGGTTAAGCAGAAATTAAGAAGAAATGTGAACTTGTTAGAGAAGCTTGTTATG
CAGGAGACTTTGTCGTGTTTAGTAGTCAATCTGTACCCAGGAAATGAAGGCTATTCTCTGATGCTCAGGG
GGAAAAATGGATCAGATTCTGAGACCATCCGATTGCCTTATGAAGAAGGAGAATTGCTTGAATACTTAGA
TGCAGAAGAATTACCTCCTATTTTGGTGGATCTCCTTGAAAAATCACAGGTTAATATTTTTCATTGTGGA
TGTGTGATAGCAGAAATACGTGACTATAGGCAGTCCAGTAATATGAAGTCTCCTGGTTACCAAAGTAGGC
ACATTCTCTTACGTCCAACAATGCAGACTTTAGTCTGTGATGTCCATTCTATAACAAGTGACAACCATAA
GTGGACCCAGGAAGACAAACTTTTGCTTGAGAGCCAGCTGATTCTAGCTACAGCAGAGCCACTGTGTCTT
GACCCATCGGTGGCAGTAGCGTGCACTGCCAATAGGCTGCTCTATAACCGGCAGAAGATGAACACTCGCC
CAATGAAACGGTGTTGGAAGAGGTACTCCAGGTCCTCCCTGAACCGCCAGCAGGACCTCTCTCACGGCCC
TCCTCCCCCTCAGCTGAGACTACTTGATTTCTTACAAAAGCGGAAGGAAAGGAAAGCAGGCCAGCATTAT
GACCTCAAAATTTCCAAAGCAGGAAATTGTGTAGACATGTGGAAACGGAGCCCCTGTAACTTGGCTGTGC
CTTCTGAAGTAGATGTGGAAAAATATGCTAAAGTGGAGGAATCCATCAAATCTGACGACTCACAGCCAAC
AATGTGGCCAGCTCATGATGTAAAAGACGATTATGTATTTGAATGTGAAGGTGGTAATCAGTATCAGAAA
ACAAAGCTGACCATCTTGCAGTCTCTTGGGGATCCACTTTACTATGGTAAAATACAGCCGTGGAAAGCAG
ATGAAGAAAATGACAGCCAAATGTCTCCATCCCACTCCTCTGCTGATGACCATTCAAACTGGTTTGTCAT
TGGGTCAAAGACTGACGCAGAGAGGGTAGTTAATCAATACCAGGAGTTGGTCCAGAATGAAGCCAAATGC
CCAGTGAAGATGTCACACAGCAGTAGTGGTTCAGCCGCTCTCAGTCCAGGGGAGGAGGCAGAACAGGCGG
AGACCTCGTCCATTCAGTCTTCAGTTTTGGGAAAGGGAGTAAAACACCGACCCCCACCCATCAAACTTCC
CTCAGGCTCAGGAAATAGTTCCTCAGGTAACTATTTCACAGCACAGCAGGCCAGCAGCTTCCTTAAATCT
CCAACTCCTCCTCCATCGTGTAAGCCAAGCCTTTCTCGGAAGTCTTCTGTGGACCTCAGTCAAGTTAGCA
TGCTTTCTCCTGCCGCCCTGTCCCCTGCCAGCTCATCCCAGAGACATGAAAGCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_019995
Insert Size 1596 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019995.3, NP_064379.1
RefSeq Size 1968 bp
RefSeq ORF 1596 bp
Locus ID 56790
UniProt ID Q7TT00
Cytogenetics 3 C
Summary Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail. Required for starvation-induced ATG9A trafficking during autophagy.UniProtKB/Swiss-Prot Function
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Resources
"NM_019995" in other vectors (4)
SKU Description Size Price
MG208520 Supt20 (tGFP-tagged) - Mouse DNA segment, Chr 3, ERATO Doi 300, expressed (D3Ertd300e) 10 ug
$694.00
MR208520 Supt20 (Myc-DDK-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) 10 ug
$289.00 $494.00
MR208520L3 Lenti ORF clone of Supt20 (Myc-DDK-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) 10 ug
$794.00
MR208520L4 Lenti ORF clone of Supt20 (mGFP-tagged) - Mouse family with sequence similarity 48, member A (Fam48a) 10 ug
$794.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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