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Trim27 (NM_009054) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208363
Trim27 (Myc-DDK-tagged) - Mouse tripartite motif-containing 27 (Trim27)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $478.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trim27
Synonyms AW538890; Gm19403; Rfp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208363 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCGGGAGCGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTTCAGTACTTCG
TGGAGCCTATGATGCTCGACTGCGGCCACAACATCTGTTGCGCGTGCCTGGCCCGCTGCTGGGGTGCCGC
GGAGACTAACGTGTCGTGTCCGCAGTGCCGGGAGACCTTCCCGCAGCGCCACATGCGACCCAACCGGCAC
CTGGCCAACGTGACCCAGCTGGTGAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGG
GCGTGTGCGAGAAGCACCGGGAGCCTCTGAAGCTGTACTGCGAGCAGGACCAGATGCCCATCTGCGTGGT
GTGCGACCGCTCGCGCGAGCACCGCGGCCACAGCGTGCTGCCCCTGGAGGAGGCGGTGGAGGGCTTCAAG
GAGCAAATCCAGAACCGACTGGACCACCTAAGAAGAGTGAAAGACTTGAAGAAGAGACGGCGGGCACAGG
GGGAGCAGGCCCGAGCTGAGCTTTTGAGCCTGACTCAGATGGAGAGGGAAAAAATTGTCTGGGAGTTTGA
GCAGCTCTATCACTCGTTGAAGGAGCATGAGTACCGCCTGTTGGCTCGCCTTGAGGAGCTCGACTTGGCC
ATCTATAACAGCATCAACGGGGCCATCACTCAGTTCTCCTGCAACATCTCCCACCTCAGTGGCCTGATTG
CCCAACTGGAAGAGAAGCAGCAGCAGCCCACGCGGGAGCTCTTACAGGACATTGGAGACACACTGAGCAG
GGCTGAAAGAATCAGGATCCCTGAGCCCTGGATCACACCCCCAGATCTACAAGAGAAAATCCATATTTTT
GCCCAAAAATGTCTGTTTTTGACAGAAAGTCTAAAACAGTTCACAGAAAAGATGCAGTCAGATATGGAGA
AGATCCAAGAGTTGAGAGAGGCTCAGTTATACTCAGTTGATGTAACCCTGGACCCAGACACAGCCTATCC
CAGTCTGATTCTTTCTGATAATCTTCGGCAAGTACGGTACAGTTACCTCCAGCAGGATCTTCCAGACAAC
CCTGAGCGATTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGTTTCATCGCTGGGAGACATTACTGGG
AGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCGGTGTGCAGAAAAGGTGG
GGTGACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCATTGTGGTATGGGAAAGAATATTGGGCTCTT
ACATCCCCAATGACTGCACTACCCCTGCGGACCCCTCTCCAGAGAGTAGGGATTTTCTTGGACTATGATG
CTGGTGAGGTTTCCTTCTACAATGTGACAGAGAGATGCCATACCTTTACTTTCTCTCACGCTACCTTCTG
TGGGCCAGTCCGGCCCTACTTCAGCCTAAGCTACTCGGGAGGGAAGAGCGCAGCTCCTTTGATCATCTGC
CCTATGAGTGGGATTGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCTCCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208363 protein sequence
Red=Cloning site Green=Tags(s)

MASGSVAECLQQETTCPVCLQYFVEPMMLDCGHNICCACLARCWGAAETNVSCPQCRETFPQRHMRPNRH
LANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEQDQMPICVVCDRSREHRGHSVLPLEEAVEGFK
EQIQNRLDHLRRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLA
IYNSINGAITQFSCNISHLSGLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIF
AQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDN
PERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWAL
TSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIIC
PMSGIDGFSGHVGNHGHSMETSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009054
ORF Size 1539 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009054.3, NP_033080.2
RefSeq Size 4204 bp
RefSeq ORF 1542 bp
Locus ID 19720
UniProt ID Q62158
Cytogenetics 13 7.67 cM
MW 58.5 kDa
Summary E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009054" in other vectors (4)
SKU Description Size Price
MC217259 Trim27 (untagged) - Mouse tripartite motif-containing 27 (Trim27), (10ug) 10 ug
$526.00
MG208363 Trim27 (tGFP-tagged) - Mouse tripartite motif protein 27 (Trim27) 10 ug
$678.00
MR208363L3 Lenti ORF clone of Trim27 (Myc-DDK-tagged) - Mouse tripartite motif-containing 27 (Trim27) 10 ug
$778.00
MR208363L4 Lenti ORF clone of Trim27 (mGFP-tagged) - Mouse tripartite motif-containing 27 (Trim27) 10 ug
$778.00

Plasmid Map for MR208363

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208363
Circular map for MR208363

file-alt Citations

Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes
Wang, Xia, Dong et al
J. Leukoc. Biol. (2018)
AI Summary: OriGene’s Mouse Trim27 cDNA (MR208363) was used to enable ectopic expression of Trim27 in mouse hematopoietic cells, allowing the researchers to investigate its role in myeloid hematopoiesis.
Snippet: Mouse Trim27 cDNA (MR208363, Origene;Rockville, MD) was cloned into a retroviral vector pMYs‐IRES‐EGFP (RTV‐021, Cell Biolabs, INC;San Diego, CA) in order to facilitate the ectopic expression of Trim27 in mouse blood cells
Showing 1-1 of 1 papers.
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