Trim27 (NM_009054) Mouse Untagged Clone

SKU
MC217259
Trim27 (untagged) - Mouse tripartite motif-containing 27 (Trim27), (10ug)
  $526.00
4 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trim27
Synonyms AW538890; Gm19403; Rfp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217259 representing NM_009054
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCGGGAGCGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTTCAGTACTTCG
TGGAGCCTATGATGCTCGACTGCGGCCACAACATCTGTTGCGCGTGCCTGGCCCGCTGCTGGGGTGCCGC
GGAGACTAACGTGTCGTGTCCGCAGTGCCGGGAGACCTTCCCGCAGCGCCACATGCGACCCAACCGGCAC
CTGGCCAACGTGACCCAGCTGGTGAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGG
GCGTGTGCGAGAAGCACCGGGAGCCTCTGAAGCTGTACTGCGAGCAGGACCAGATGCCCATCTGCGTGGT
GTGCGACCGCTCGCGCGAGCACCGCGGCCACAGCGTGCTGCCCCTGGAGGAGGCGGTGGAGGGCTTCAAG
GAGCAAATCCAGAACCGACTGGACCACCTAAGAAGAGTGAAAGACTTGAAGAAGAGACGGCGGGCACAGG
GGGAGCAGGCCCGAGCTGAGCTTTTGAGCCTGACTCAGATGGAGAGGGAAAAAATTGTCTGGGAGTTTGA
GCAGCTCTATCACTCGTTGAAGGAGCATGAGTACCGCCTGTTGGCTCGCCTTGAGGAGCTCGACTTGGCC
ATCTATAACAGCATCAACGGGGCCATCACTCAGTTCTCCTGCAACATCTCCCACCTCAGTGGCCTGATTG
CCCAACTGGAAGAGAAGCAGCAGCAGCCCACGCGGGAGCTCTTACAGGACATTGGAGACACACTGAGCAG
GGCTGAAAGAATCAGGATCCCTGAGCCCTGGATCACACCCCCAGATCTACAAGAGAAAATCCATATTTTT
GCCCAAAAATGTCTGTTTTTGACAGAAAGTCTAAAACAGTTCACAGAAAAGATGCAGTCAGATATGGAGA
AGATCCAAGAGTTGAGAGAGGCTCAGTTATACTCAGTTGATGTAACCCTGGACCCAGACACAGCCTATCC
CAGTCTGATTCTTTCTGATAATCTTCGGCAAGTACGGTACAGTTACCTCCAGCAGGATCTTCCAGACAAC
CCTGAGCGATTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGTTTCATCGCTGGGAGACATTACTGGG
AGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCGGTGTGCAGAAAAGGTGG
GGTGACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCATTGTGGTATGGGAAAGAATATTGGGCTCTT
ACATCCCCAATGACTGCACTACCCCTGCGGACCCCTCTCCAGAGAGTAGGGATTTTCTTGGACTATGATG
CTGGTGAGGTTTCCTTCTACAATGTGACAGAGAGATGCCATACCTTTACTTTCTCTCACGCTACCTTCTG
TGGGCCAGTCCGGCCCTACTTCAGCCTAAGCTACTCGGGAGGGAAGAGCGCAGCTCCTTTGATCATCTGC
CCTATGAGTGGGATTGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCTCCGT
GA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_009054
Insert Size 1542 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009054.3, NP_033080.2
RefSeq Size 4204 bp
RefSeq ORF 1542 bp
Locus ID 19720
UniProt ID Q62158
Cytogenetics 13 7.67 cM
Summary E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.UniProtKB/Swiss-Prot Function
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"NM_009054" in other vectors (4)
SKU Description Size Price
MG208363 Trim27 (tGFP-tagged) - Mouse tripartite motif protein 27 (Trim27) 10 ug
$678.00
MR208363 Trim27 (Myc-DDK-tagged) - Mouse tripartite motif-containing 27 (Trim27) 10 ug
$289.00 $478.00
MR208363L3 Lenti ORF clone of Trim27 (Myc-DDK-tagged) - Mouse tripartite motif-containing 27 (Trim27) 10 ug
$778.00
MR208363L4 Lenti ORF clone of Trim27 (mGFP-tagged) - Mouse tripartite motif-containing 27 (Trim27) 10 ug
$778.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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