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Glis2 (NM_031184) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208349
Glis2 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 2 (Glis2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $486.00
In Stock*
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Other products for "Glis2 or NM_031184"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Glis2
Synonyms Gli5; Klf16; Nkl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208349 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTCCTTGGACGAGCCCCTCGACCTAAAGCTGAGCATCACCAAGCTCCGAGCGGCAAGAGAGAAGA
GAGAAAGGACACTGGGTGTGGTCCGGCATCATGCTTTGCATCGAGAGCTGGGCCTGGTGGACGATAGCCC
CGCCCCTGGCTCCCCAGGCTCTCCACCACCAGGTTTCCTGCTGAACCCCAAATTCCCTGAGAAGGTGGAC
GGACGCTTTTCTGCAGCCCCCTTGGTGGACCTCAGCTTGTCACCACCCTCTGGACTGGACTCTCCCAATG
GCAGCAGCTCCCTGTCTCCTGAGTGCCAGGGCAATGGGGACTTGCCTCCACTGCCTACTGCTGTGGATTT
CCAGCCACTTCGCTATTTGGATGGTGTCCCCAGTTCCTTCCAGTTCTTCTTGCCCCTGGGTTCTGGTGGG
GCTCTGCACCTACCTGCTTCCTCCTTCCTTCCCCCCCCCAAGGACAAGTGCCTCTCACCAGAGCTGCCCC
TGGCCAAGCAGCTGGTGTGTCGATGGGCCAAGTGTAACCAGCTCTTTGAGCTCCTCCAAGACCTGGTTGA
CCATGTCAACGACCATCATGTCAAGCCTGAACAGGATGCTCGATACTGCTGTCATTGGGAGGGCTGCGCC
CGCCATGGCCGTGGCTTCAATGCCAGGTACAAGATGCTCATTCACATCCGGACACACACCAACGAGAAGC
CGCACCGATGCCCCACCTGTAACAAGAGCTTCTCCCGCCTGGAGAACCTGAAGATCCACAACCGCTCCCA
CACAGGTGAGAAGCCCTACGTCTGCCCCTATGAGGGCTGCAACAAGCGTTACTCCAACTCCAGTGACCGC
TTCAAGCACACCCGTACCCACTACGTAGACAAGCCCTACTACTGCAAGATGCCCGGCTGTCACAAGCGTT
ACACGGACCCCAGTTCACTGCGCAAACACATCAAGGCCCATGGCCACTTTGTGTCACATGAGCAGCAGGA
GCTCCTGCAGCTGCGCCCACCCCCTAAACCACCACTGCCCACTCCTGACAGTGGCTCCTATGTCAGCGGG
GCTCAGATCATCATCCCGAACCCTGCTGCCCTTTTTGGAGGCCCCAGTCTGCCTGGCCTGCCATTACCTC
TACCTCCTGGCCCCCTTGACCTCAGTGCTCTGGCCTGTGGCAATGGTGGAGGTGGGGGTGGGGGTATTGG
CCCTGGGCTGCCAGGCTCTGTTCTGCCCCTCAATCTGGCCAAGAACCCGCTGTTGCCCTCACCCTTTGGG
GCTGGTGGACTAGGCCTGCCTGTGGTTTCTCTCCTGGGTGGCTCTGCCGGTAGCAAGGCCGAGGGGGAGA
AGGGTCGTGGGTCAGTACCTGCCAGGGTCCTGGGCCTGGAAGATCACAAGACGCCCCTGGAAAGGACGGA
GCGCAGCCGCTCCCGGCCAAGCCCTGATGGACTCCCTTTGCTCCCAGGCACTGTACTGGACCTGTCCACA
GGCAACTCAGCAGCCAGCAGTCCAGAGGTGTTAACTCCTGGCTGGGTGGTCATTCCACCAGGGTCTGTGC
TGCTCAAACCAGCTGTGGTAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208349 protein sequence
Red=Cloning site Green=Tags(s)

MHSLDEPLDLKLSITKLRAAREKRERTLGVVRHHALHRELGLVDDSPAPGSPGSPPPGFLLNPKFPEKVD
GRFSAAPLVDLSLSPPSGLDSPNGSSSLSPECQGNGDLPPLPTAVDFQPLRYLDGVPSSFQFFLPLGSGG
ALHLPASSFLPPPKDKCLSPELPLAKQLVCRWAKCNQLFELLQDLVDHVNDHHVKPEQDARYCCHWEGCA
RHGRGFNARYKMLIHIRTHTNEKPHRCPTCNKSFSRLENLKIHNRSHTGEKPYVCPYEGCNKRYSNSSDR
FKHTRTHYVDKPYYCKMPGCHKRYTDPSSLRKHIKAHGHFVSHEQQELLQLRPPPKPPLPTPDSGSYVSG
AQIIIPNPAALFGGPSLPGLPLPLPPGPLDLSALACGNGGGGGGGIGPGLPGSVLPLNLAKNPLLPSPFG
AGGLGLPVVSLLGGSAGSKAEGEKGRGSVPARVLGLEDHKTPLERTERSRSRPSPDGLPLLPGTVLDLST
GNSAASSPEVLTPGWVVIPPGSVLLKPAVVN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031184
ORF Size 1563 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031184.3, NP_112461.2
RefSeq Size 3619 bp
RefSeq ORF 1566 bp
Locus ID 83396
UniProt ID Q8VDL9
Cytogenetics 16 2.45 cM
MW 55.8 kDa
Summary Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_031184" in other vectors (4)
SKU Description Size Price
MC204306 Glis2 (untagged) - Mouse GLIS family zinc finger 2 (Glis2), (10ug) 10 ug
$486.00
MG208349 Glis2 (tGFP-tagged) - Mouse GLIS family zinc finger 2 (Glis2) 10 ug
$686.00
MR208349L3 Lenti ORF clone of Glis2 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 2 (Glis2) 10 ug
$786.00
MR208349L4 Lenti ORF clone of Glis2 (mGFP-tagged) - Mouse GLIS family zinc finger 2 (Glis2) 10 ug
$786.00

Plasmid Map for MR208349

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208349
Circular map for MR208349

file-alt Citations

Identification of a Crosstalk among TGR5, GLIS2, and TP53 Signaling Pathways in the Control of Undifferentiated Germ Cell Homeostasis and Chemoresistance
Thirouard, Holota, Monrose et al
Adv Sci (Weinh) (2022) 9 (17), e2200626 DOI: 10.1002/advs.202200626
Cited by 15
AI Summary: In this study, OriGene products including the GLIS2 (MR215251) and TP53 (MR204079) expression plasmids were used to investigate the molecular interactions among TGR5, GLIS2, and TP53 signaling pathways in regulating spermatogonial stem cell homeostasis and chemoresistance.
FigureFigureFigure
Showing 1-1 of 1 papers.
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