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Glis2 (NM_031184) Mouse Untagged Clone

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SKU
MC204306
Glis2 (untagged) - Mouse GLIS family zinc finger 2 (Glis2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $486.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Glis2
Synonyms Gli5; Klf16; Nkl
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC021517
CCACGCGTCCGGCCGGAACCCGCAGCCGGAGCCAGTCAGCCCGGCAGCCGAGGTTCCTGCCTCAAGACCA GTTGGGTACATCTTTGACACGCCCTCCCACCATGCACTCCTTGGACGAGCCCCTCGACCTAAAGCTGAGC ATCACCAAGCTCCGAGCGGCAAGAGAGAAGAGAGAAAGGACACTGGGTGTGGTCCGGCATCATGCTTTGC ATCGAGAGCTGGGCCTGGTGGACGATAGCCCCGCCCCTGGCTCCCCAGGCTCTCCACCACCAGGTTTCCT GCTGAACCCCAAATTCCCTGAGAAGGTGGACGGACGCTTTTCTGCAGCCCCCTTGGTGGACCTCAGCTTG TCACCACCCTCTGGACTGGACTCTCCCAATGGCAGCAGCTCCCTGTCTCCTGAGTGCCAGGGCAATGGGG ACTTGCCTCCACTGCCTACTGCTGTGGATTTCCAGCCACTTCGCTATTTGGATGGTGTCCCCAGTTCCTT CCAGTTCTTCTTGCCCCTGGGTTCTGGTGGGGCTCTGCACCTACCTGCTTCCTCCTTCCTTCCCCCCCCC AAGGACAAGTGCCTCTCACCAGAGCTGCCCCTGGCCAAGCAGCTGGTGTGTCGATGGGCCAAGTGTAACC AGCTCTTTGAGCTCCTCCAAGACCTGGTTGACCATGTCAACGACCATCATGTCAAGCCTGAACAGGATGC TCGATACTGCTGTCATTGGGAGGGCTGCGCCCGCCATGGCCGTGGCTTCAATGCCAGGTACAAGATGCTC ATTCACATCCGGACACACACCAACGAGAAGCCGCACCGATGCCCCACCTGTAACAAGAGCTTCTCCCGCC TGGAGAACCTGAAGATCCACAACCGCTCCCACACAGGTGAGAAGCCCTACGTCTGCCCCTATGAGGGCTG CAACAAGCGTTACTCCAACTCCAGTGACCGCTTCAAGCACACCCGTACCCACTACGTAGACAAGCCCTAC TACTGCAAGATGCCCGGCTGTCACAAGCGTTACACGGACCCCAGTTCACTGCGCAAACACATCAAGGCCC ATGGCCACTTTGTGTCACATGAGCAGCAGGAGCTCCTGCAGCTGCGCCCACCCCCTAAACCACCACTGCC CACTCCTGACAGTGGCTCCTATGTCAGCGGGGCTCAGATCATCATCCCGAACCCTGCTGCCCTTTTTGGA GGCCCCAGTCTGCCTGGCCTGCCATTACCTCTACCTCCTGGCCCCCTTGACCTCAGTGCTCTGGCCTGTG GCAATGGTGGAGGTGGGGGTGGGGGTATTGGCCCTGGGCTGCCAGGCTCTGTTCTGCCCCTCAATCTGGC CAAGAACCCGCTGTTGCCCTCACCCTTTGGGGCTGGTGGACTAGGCCTGCCTGTGGTTTCTCTCCTGGGT GGCTCTGCCGGTAGCAAGGCCGAGGGGGAGAAGGGTCGTGGGTCAGTACCTGCCAGGGTCCTGGGCCTGG AAGATCACAAGACGCCCCTGGAAAGGACGGAGCGCAGCCGCTCCCGGCCAAGCCCTGATGGACTCCCTTT GCTCCCAGGCACTGTACTGGACCTGTCCACAGGCAACTCAGCAGCCAGCAGTCCAGAGGTGTTAACTCCT GGCTGGGTGGTCATTCCACCAGGGTCTGTGCTGCTCAAACCAGCTGTGGTAAACTGAAACTGGGCAACCC ATCTACCAGCTGTGACAGCCCAGCCACCTACCGTGGGCTCATCCCCCGACGAACAGAAACTCTTCTGCGA AATAGCAATAATATCCTACTGCCCCAGGGCCAAGCTGCAGCCCCAGACAAGCTGGGTGGCAAGGTGGCAA GGATGGTGCTAGAAGGTCTGTGCTGGCCTCCTGGCTCCAAAGTGAGGACTTGGCTTGGACCTGCTGTCCA AGAAGAGCCATTCTCTTGGGTGCTAAGGCCTCAATCACTTCCATTTCCCTAGTCTGTAGTCTGGGTGAGG CCTTTGCCTGTCCTCCCAGGACGCTCAGTCCTGCCTCTTGCTCTGGTGCGTTCCTCTCTATGGCCACCCT GGCTGGGCAGGGTTCCAGTCCTCCCTGCCTTACCTACTTGTCAGCTAGGAGCCCCCAGTGATCATAGATA GGCCCACACTTGGGAATTCCTTAGACCCCTTTCCCTCTGGTCCACCTCTAGAGGGAGAGCAAGACAGACA CTGCTGCCTTCTCCTGCCTGCTGCTATGACAAGGTGCTTCTCTGCTTGCTTAGCAGAGAAGCCTGCTCTG CCAGCACTTCTCTTTCCTGCCAGAGGGTTGGCAATACTTTCCAAAGAGGAGGGGTAGCTGACCACTCAAC CTTCTCCTTGGGGATGTGCAGGCAGGTACAAGGGTCACTGTGCTGACCTGGCCCGGCTTGGCCTGGCCTG AGCAGTGTTACTCTGCAGAAGCTTATAAGGCACTAGTCTACACCACGTGGGCTCTCAAGCTTGGGACACA GCTACAGGTTTGGGGGCACCAAGTAATGGGATGCAGGGAAGAAGACTCATTGGAAAGGCGCTTTCTAGAA AGCACTGACTGAAGCCCCTGAGTGTTAAGGGAGGCTAGGCAGCCAAGCACAGCATTAGCTTGGGAAAAAG CTAGCTAGAGAAAGGCCTGGCCATCTGGCAGGGTTCTTTCCTGACTCTAGTTGAACCCTTTTCTTGGTGC TGCCTCTCTTGCCCATGAGCTCTGCCCAAGCCAGCGACCCCAGGGCTTGTGCAACAAACCAAGGACATTG GAGGACTGTGTGCTCCATAGCTCCAAAAGGCTAGCTGCCCCAGCTTGGGCTTCTTGCTGTCCCCTGTACT ATGCTGCAGTGGCTAAGCCCCTCATGTCCCCATCTGTAGAACAAGAGAGCTGCTGAGGTGCTAGTGGGTC CTTGGCTCTGTGGTTCCTCTGAGCAGTTCTGTCTAGTCTTTTTCTCAGACTGCCTTTCTGTTTCAGAAGG GTCACCCTTCCCTAACCTACCTGACTGCTGGCCTTAGAACCCCAGCCCTGATGGGGCTCGGCTGTGCTGG CTCTCCTTGATGGGGCTGTGAGCAAGTTAAGGAGCTGGTCTCCTACCTTTGGGTCTGCCTAGGATCCAAG CAACTCCTGGCTCTATTAGGGTCCTAGGCCTTAAGTCCCTGGCTGGGGGATTTGGTTTGAGACTCAAGCC AAGGAGAAGAACAGAGCACTGGACATTAGCTCAGCATGTGACTCAAGTGATAGACCAGGCTCAGAAGGGC TGGTTACTTCCTGTCAGTTTGTTGCACATTCCAGGATTCAGTATTTTAACAGGTTCTAAGTGCCTTTCTC TTGTAGTGTATGGTTTTCCTCCTTCTGGCTCCATGGCTGTTAGCATAGAGTTTAAAAAAAGAGAGATAAG CTAATGACTATAACAATATATTCCTCCATGGGAGAGGAAGTTTATACAGAAACAATAAAGTGAGTTGCAA AGATGGTTTCTGCAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_031184
Insert Size 1566 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC021517, AAH21517
RefSeq Size 3460 bp
RefSeq ORF 1566 bp
Locus ID 83396
UniProt ID Q8VDL9
Cytogenetics 16 2.45 cM
Summary Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.UniProtKB/Swiss-Prot Function
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"NM_031184" in other vectors (4)
SKU Description Size Price
MG208349 Glis2 (tGFP-tagged) - Mouse GLIS family zinc finger 2 (Glis2) 10 ug
$686.00
MR208349 Glis2 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 2 (Glis2) 10 ug
$289.00 $486.00
MR208349L3 Lenti ORF clone of Glis2 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 2 (Glis2) 10 ug
$786.00
MR208349L4 Lenti ORF clone of Glis2 (mGFP-tagged) - Mouse GLIS family zinc finger 2 (Glis2) 10 ug
$786.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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