Bin1 (NM_001083334) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR207643
Bin1 (Myc-DDK-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Bin1 |
| Synonyms | ALP-1; Amphl; BRAMP-2; SH3P9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001083334 |
| ORF Size | 1431 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001083334.1, NP_001076803.1 |
| RefSeq Size | 2135 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 30948 |
| Cytogenetics | 18 18.01 cM |
| MW | 52.8 kDa |
| Summary | Is a key player in the control of plasma membrane curvature, and membrane shaping and remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:12183633). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:12668730, PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001083334" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC202551 | Bin1 (untagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2, (10ug) | 10 ug |
$457.00
|
|
| MG207643 | Bin1 (tGFP-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2 | 10 ug |
$657.00
|
|
| MR207643L3 | Lenti ORF clone of Bin1 (Myc-DDK-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2 | 10 ug |
$757.00
|
|
| MR207643L4 | Lenti ORF clone of Bin1 (mGFP-tagged) - Mouse bridging integrator 1 (Bin1), transcript variant 2 | 10 ug |
$757.00
|
|
Citations
BIN1 favors the spreading of Tau via extracellular vesicles
Crotti, Sait, McAvoy et al
Sci Rep (2019) 9 (1), 9477 DOI: 10.1038/s41598-019-45676-0
Crotti, Sait, McAvoy et al
Sci Rep (2019) 9 (1), 9477 DOI: 10.1038/s41598-019-45676-0
AI Summary: OriGene products, specifically the plasmids expressing human Tau 2N4R (Cat#: RC213312) and murine Bin1 transcript variant 2 (Cat#: MR207643), were used to investigate the roles of BIN1 and Tau in extracellular vesicle-mediated Tau spreading in Alzheimer's disease models.
Snippet: pLVX Ef1a Ires puro vector from Clontech was cut with EcoRI/SpeI. Plasmids expressing human Tau 2N4R (Cat#: RC213312) and murine Bin1 transcript variant 2 (Cat#:MR207643) are commercially available (Origene). An insert encoding 2N4R human Tau with a P301L mutation and YFP at the C-terminus was synthesized as a linear DNA string, and cloned into pLVX Ires-Puro lentiviral plasmid from Clontech usin
Showing 1-1 of 1 papers.
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